RIPAb+™ EED - RIP Validated Antibody and Primer Set | EMD Millipore 03-196 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RIPAb+™ EED - RIP Validated Antibody and Primer Set

catalog :

03-196

quantity :

10 assays

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunoprecipitation

citations: 1

Reference
Holdt L, Hoffmann S, Sass K, Langenberger D, Scholz M, Krohn K, et al. Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell functions through trans-regulation of gene networks. PLoS Genet. 2013;9:e1003588 pubmed publisher

product information

Catalog Number :

03-196

Subcategory :

Epigenetics & Nuclear Function

Product Name :

RIPAb+™ EED - RIP Validated Antibody and Primer Set

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

O75530

Host Name :

Mouse

Antigen :

EED

Conjugate :

Ascites

Isotype :

IgG2aκ

Product Description :

RIPAb+™ EED - RIP Validated Antibody and Primer Set

Cross Reactivity :

Human;Mouse

Background :

Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.

ALT Names :

WD protein associating with integrin cytoplasmic tails 1 ;embryonic ectoderm development

Immunogen :

Recombinant fusion protein of full length murine EED tagged with MBP.

Specificity :

This antibody recognizes mouse EED, (~30-65 kDa).

Package Size :

10 assays

Uses :

RNA Binding Protein Immunoprecipitation (RIP);Western Blotting;Immunoprecipitation

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.