RIPAb+ Hexim 1 - RIP Validated Antibody and Primer Set | EMD Millipore 03-177 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RIPAb+ Hexim 1 - RIP Validated Antibody and Primer Set

catalog :

03-177

quantity :

10 assays

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, chimpanzee

application :

western blot, immunoprecipitation

citations: 1

Reference
Hu J, Lu J, Lian G, Zhang J, Hecht J, Sheen V. Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling. PLoS ONE. 2014;9:e89352 pubmed publisher

product information

Catalog Number :

03-177

Subcategory :

Epigenetics & Nuclear Function

Product Name :

RIPAb+ Hexim 1 - RIP Validated Antibody and Primer Set

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

O94992

Host Name :

Rabbit

Antigen :

Hexim 1

Conjugate :

Affinity Purified

Product Description :

RIPAb+ Hexim 1 - RIP Validated Antibody and Primer Set

Cross Reactivity :

Human;Chimpanzee;Rhesus Macaque

Background :

Hexim1 is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. Hexim1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), Hexim1, Hexim2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. Hexim1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. Hexim1 is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen.

ALT Names :

Cardiac lineage protein 1;Estrogen down-regulated gene 1 protein;Hexamethylene bis-acetamide-inducible protein 1;Menage a quatre protein 1;hexamethylene bis-acetamide inducible 1;hexamethylene bisacetamide-inducible protein;hexamethylene-bis-acetamide-inducible transcript 1;hexamthylene bis-acetamide inducible 1;menage a quatre 1

Immunogen :

KLH-conjugated linear peptide corresponding to human Hexim 1 at and around the N-terminus.

Specificity :

This antibody recognizes Hexim1 at the N-terminus.

Package Size :

10 assays

Uses :

RNA Binding Protein Immunoprecipitation (RIP);Western Blotting;Immunoprecipitation

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.