RIPAb+ Ago2 - RIP Validated Antibody and Primer Set | EMD Millipore 03-110 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RIPAb+ Ago2 - RIP Validated Antibody and Primer Set

catalog :

03-110

quantity :

10 assays

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, bovine

application :

western blot, immunoprecipitation, chromatin immunoprecipitation, RNA immunoprecipitation

citations: 3

Published Application/Species/Sample/Dilution	Reference
RNA immunoprecipitation; human; fig 8	Morales HernÃ¡ndez A, GonzÃ¡lez Rico F, RomÃ¡n A, Rico Leo E, Alvarez Barrientos A, SÃ¡nchez L, et al. Alu retrotransposons promote differentiation of human carcinoma cells through the aryl hydrocarbon receptor. Nucleic Acids Res. 2016;44:4665-83 pubmed publisher
chromatin immunoprecipitation; human	Iosue I, Quaranta R, Masciarelli S, Fontemaggi G, Batassa E, Bertolami C, et al. Argonaute 2 sustains the gene expression program driving human monocytic differentiation of acute myeloid leukemia cells. Cell Death Dis. 2013;4:e926 pubmed publisher
	Masciarelli S, Quaranta R, Iosue I, Colotti G, Padula F, Varchi G, et al. A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4. ACS Chem Biol. 2014;9:1674-9 pubmed publisher

product information

Catalog Number :

03-110

Subcategory :

Epigenetics & Nuclear Function

Product Name :

RIPAb+ Ago2 - RIP Validated Antibody and Primer Set

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q9UKV8

Host Name :

Mouse

Antigen :

Ago2

Conjugate :

Ascites

Isotype :

IgG1κ

Product Description :

RIPAb+ Ago2 - RIP Validated Antibody and Primer Set

Cross Reactivity :

Human;Bovine

Background :

Ago2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).

ALT Names :

Argonaute-2;Eukaryotic translation initiation factor 2C;Slicer protein;Piwi domain protein

Immunogen :

KLH-conjugated, synthetic peptide containing the sequence YSGAGPALAPPAPPPPIQG.

Specificity :

Recognizes Ago2.

Package Size :

10 assays

Uses :

RNA Binding Protein Immunoprecipitation (RIP);Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance