RIPAb+ CUGBP1 - RIP Validated Antibody and Primer Set | EMD Millipore 03-104 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RIPAb+ CUGBP1 - RIP Validated Antibody and Primer Set

catalog :

03-104

quantity :

10 assays

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, bovine

application :

western blot, immunoprecipitation

citations: 1

Reference
Popovitchenko T, Park Y, Page N, Luo X, Krsnik Z, Liu Y, et al. Translational derepression of Elavl4 isoforms at their alternative 5' UTRs determines neuronal development. Nat Commun. 2020;11:1674 pubmed publisher

product information

Catalog Number :

03-104

Subcategory :

Epigenetics & Nuclear Function

Product Name :

RIPAb+ CUGBP1 - RIP Validated Antibody and Primer Set

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q92879

Host Name :

Mouse

Antigen :

CUGBP1

Conjugate :

Purified

Isotype :

IgG1κ

Product Description :

RIPAb+ CUGBP1 - RIP Validated Antibody and Primer Set

Cross Reactivity :

Human;Mouse;Rat;Bovine;Pig;Rabbit

Background :

Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG)n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis. HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG)8 oligonucleotides in vitro. CUG-BP1 is the major (CUG)8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo. In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3'-UTR.

ALT Names :

CUG triplet repeat, RNA binding protein 1

Immunogen :

The CUGBP1 antibody is made against full-length GST fusion protein corresponding to human CUGBP1, also known as CUG triplet repeat, RNA binding protein 1 or heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50.

Specificity :

CUGBP1

Package Size :

10 assays

Uses :

RNA Binding Protein Immunoprecipitation (RIP);Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.