product summary
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company name :
Dianova
product type :
antibody
product name :
Anti-HIS Epitope-Tag (rec) from Mouse (Clone: 13/45/31)
catalog :
DIA-900
quantity :
200 µg
price :
248 EUR plus VAT
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
13/45/31
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - frozen section
more info or order :
Dianova product webpage
citations: 21
Reference
Khodorova N, Rutledge D, Oberli M, Mathiron D, Marcelo P, Benamouzig R, et al. Urinary Metabolomics Profiles Associated to Bovine Meat Ingestion in Humans. Mol Nutr Food Res. 2018;:e1700834 pubmed publisher
Nausch H, Broer I. Cyanophycinase CphE from P. alcaligenes produced in different compartments of N. benthamiana degrades high amounts of cyanophycin in plant extracts. Appl Microbiol Biotechnol. 2017;101:2397-2413 pubmed publisher
Fernández Ulibarri I, Hammer K, Arndt M, Kaufmann J, Dorer D, Engelhardt S, et al. Genetic delivery of an immunoRNase by an oncolytic adenovirus enhances anticancer activity. Int J Cancer. 2015;136:2228-40 pubmed publisher
Ponnusamy R, Mesters J, Ziebuhr J, Moll R, Hilgenfeld R. Non structural proteins 8 and 9 of human coronavirus 229E. Adv Exp Med Biol. 2006;581:49-54 pubmed
Muller D, Karle A, Meissburger B, Höfig I, Stork R, Kontermann R. Improved pharmacokinetics of recombinant bispecific antibody molecules by fusion to human serum albumin. J Biol Chem. 2007;282:12650-60 pubmed
Offner S, Hofmeister R, Romaniuk A, Kufer P, Baeuerle P. Induction of regular cytolytic T cell synapses by bispecific single-chain antibody constructs on MHC class I-negative tumor cells. Mol Immunol. 2006;43:763-71 pubmed
Reininger R, Swoboda I, Bohle B, Hauswirth A, Valent P, Rumpold H, et al. Characterization of recombinant cat albumin. Clin Exp Allergy. 2003;33:1695-702 pubmed
Le Gall F, Reusch U, Moldenhauer G, Little M, Kipriyanov S. Immunosuppressive properties of anti-CD3 single-chain Fv and diabody. J Immunol Methods. 2004;285:111-27 pubmed
Stork R, Zettlitz K, Muller D, Rether M, Hanisch F, Kontermann R. N-glycosylation as novel strategy to improve pharmacokinetic properties of bispecific single-chain diabodies. J Biol Chem. 2008;283:7804-12 pubmed publisher
Roobol Bóza M, Dolby V, Doverskog M, Barrefelt A, Lindqvist F, Oppermann U, et al. Membrane protein isolation by in situ solubilization, partitioning and affinity adsorption in aqueous two-phase systems. Purification of the human type 1 11beta-hydroxysteroid dehydrogenase. J Chromatogr A. 2004;1043:217-23 pubmed
Fachiroh J, Stevens S, Haryana S, Middeldorp J. Combination of Epstein-Barr virus scaffold (BdRF1/VCA-p40) and small capsid protein (BFRF3/VCA-p18) into a single molecule for improved serodiagnosis of acute and malignant EBV-driven disease. J Virol Methods. 2010;169:79-86 pubmed publisher
Unverdorben F, Färber Schwarz A, Richter F, Hutt M, Kontermann R. Half-life extension of a single-chain diabody by fusion to domain B of staphylococcal protein A. Protein Eng Des Sel. 2012;25:81-8 pubmed publisher
Fournier P, Aigner M, Schirrmacher V. Targeting of IL-2 and GM-CSF immunocytokines to a tumor vaccine leads to increased anti-tumor activity. Int J Oncol. 2011;38:1719-29 pubmed publisher
Messerschmidt S, Kolbe A, Muller D, Knoll M, Pleiss J, Kontermann R. Novel single-chain Fv' formats for the generation of immunoliposomes by site-directed coupling. Bioconjug Chem. 2008;19:362-9 pubmed
Boehle A, Kurdow R, Schulze M, Kliche U, Sipos B, Soondrum K, et al. Human endostatin inhibits growth of human non-small-cell lung cancer in a murine xenotransplant model. Int J Cancer. 2001;94:420-8 pubmed
Walchli S, Espanel X, Hooft van Huijsduijnen R. Sap-1/PTPRH activity is regulated by reversible dimerization. Biochem Biophys Res Commun. 2005;331:497-502 pubmed
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Schulte M, Reiss K, Lettau M, Maretzky T, Ludwig A, Hartmann D, et al. ADAM10 regulates FasL cell surface expression and modulates FasL-induced cytotoxicity and activation-induced cell death. Cell Death Differ. 2007;14:1040-9 pubmed
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product information
Catalog Number :
DIA-900
Product Name :
Anti-HIS Epitope-Tag (rec) from Mouse (Clone: 13/45/31)
Product Type :
Antibody
Host Species :
Mouse
Clonality :
Monoclonal
Conjugation :
nonconjugated
Clone Name :
13/45/31
Isotype :
IgG1
Light Chain :
kappa
Size :
200 µg
List Price :
248 EUR plus VAT
Product Description :
The mouse monoclonal anti-(His)6-tag antibody, clone 13/45/31-2, specifically detects any kind of histidine-tagged proteins in cells and complex cellular lysates. This monoclonal antibody specifically reacts with recombinant proteins containing an epitope of at least 6 histidine residues, located at the N-terminus, C-terminus or at both termini. A higher number of histidine residues leads to an increased binding affinity of the antibody, e.g. an expression product with 10 histidine residues increases the affinity about 10- to 20-fold. In most cases additional flanking amino acids are not required for antibody binding, therefore enabling the choice of many different expression vectors on the only condition that the epitope is spatially available.
Background :
Recombinant proteins are utilized for various purposes in molecular biology such as the production of antibodies for investigation of the mechanism of protein-nucleic acid or protein-protein interactions. Many prokaryotic expression vectors have been established enabling synthesis of the protein of interest as a fusion with a peptide, thus facilitating purification. Expression of recombinant proteins in E. coli as a fusion protein with neighbouring histidine residues is one of the most popular methods, because the proteins have useful attributes. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbant.
ALTnames :
histag;histidin tag;hexahistidine;his6;histidine;polyhistidine
Immunogen :
Recombinant (HIS)6-p53 protein
Specificity :
Recognizes N-terminal, C-terminal or internal HIS-tagged fusion proteins with at least 6 histidine residues
Purity :
Purified
Preservative :
0.05% NaN3
Concentration :
0.2 mg/ml
Application Summary :
ELISA;FACS;IHC-F;IF;IP;WB
more info or order :
Dianova product webpage
company information
Dianova
Warburgstr. 45
D-20354 Hamburg
info@dianova.de
www.dianova.de
49 40 45 06 73 30
headquarters: Germany
Since its foundation in 1982, dianova GmbH provides professionals in Life Sciences with antibodies, immunoassays, and products for molecular biology. More than 30 years of grown expertise give rise to a fundamental knowledge about biochemical applications and a profound understanding of customer needs in research and routine diagnostic laboratories of universities, hospitals and the pharmaceutical industry.
Since its foundation in 1982, dianova GmbH provides professionals in Life Sciences with antibodies, immunoassays, and products for molecular biology. More than 30 years of grown expertise give rise to a fundamental knowledge about biochemical applications and a profound understanding of customer needs in research and routine diagnostic laboratories of universities, hospitals and the pharmaceutical industry.
In addition to distributing Life Science and Diagnostic Products in the German speaking region dianova manufactures and markets a series of antibodies with unique properties on a worldwide basis under the brand of optistain. To this end dianova has successfully established a distribution network in more than 25 countries.
At dianova quality is of paramount importance and subject to strict control. Internal controls and audits are carried out in addition to on-site inspections by independent, external inspection companies. In 2003 the TÜV Rheinland/Berlin/Brandenburg successfully completed the first comprehensive certification process. Since then dianova has been certified pursuant to the international DIN EN ISO 9001:2008 standard.