product summary
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company name :
Dianova
product type :
antibody
product name :
Anti-CD8a (Ms) from Rat (Clone: GHH8) for mouse FFPE tissue
catalog :
DIA-808
quantity :
200 µl
price :
345 EUR plus VAT
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
GHH8
reactivity :
mouse
application :
immunohistochemistry - paraffin section
more info or order :
Dianova product webpage
citations: 3
Reference
Rech A, Dada H, Kotzin J, Henao Mejia J, Minn A, Twyman Saint Victor C, et al. Radiotherapy and CD40 Activation Separately Augment Immunity to Checkpoint Blockade in Cancer. Cancer Res. 2018;78:4282-4291 pubmed publisher
Kuczynski E, Krueger J, Chow A, Xu P, Man S, Sundaravadanam Y, et al. Impact of Chemical-Induced Mutational Load Increase on Immune Checkpoint Therapy in Poorly Responsive Murine Tumors. Mol Cancer Ther. 2018;17:869-882 pubmed publisher
Wüstner S, Anderl F, Wanisch A, Sachs C, Steiger K, Nerlich A, et al. Helicobacter pylori γ-glutamyl transferase contributes to colonization and differential recruitment of T cells during persistence. Sci Rep. 2017;7:13636 pubmed publisher
image
image 1 :
Dianova DIA-808 image 1
The monoclonal antibody clone GHH8 specifically stains CD8a in standard formalin-fixed paraffin-embedded mouse spleen.
product information
Catalog Number :
DIA-808
Product Name :
Anti-CD8a (Ms) from Rat (Clone: GHH8) for mouse FFPE tissue
Product Type :
Antibody
Host Species :
Rat
Clonality :
Monoclonal
Conjugation :
nonconjugated
Clone Name :
GHH8
Isotype :
IgG2a
Light Chain :
kappa
Size :
200 µl
List Price :
345 EUR plus VAT
Product Description :
Antibody clone GHH8 has been developed and validated for the detection of murine CD8a in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). Clone GHH8 identifies cytotoxic and regulatory T-cells (also known as suppressor T-cells) in murine FFPE tissue by IHC.
CrossReactivity :
does not cross react with human
Background :
CD8 is a marker for T-cells with suppressor and cytotoxic activity. Cluster of Differentiation 8 (CD8) is also found on the surface of some natural killer cells, most thymocytes, some T-cell lymphomas and large granular lymphocyte leukemias. Antigen recognition and subsequent T-cell activation is controlled by the interaction between TCR (T-cell receptor) and pMHC (peptide-major histocompatibility complexe). T-cell activation is often enhanced by a close cooperation of the TCR with two different co-receptors: CD8 and CD4. CD8 was first discovered as a cell surface marker in mice and was used to discriminate CD8+ CTLs and CD4+ Th cells. CD8a is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). CD8 alpha beta is expressed on the majority of cytotoxic T-lymphocytes (CTLs). This CD8 heteromeric proteins (with approx. 32-34 KDa each monomer) are physically associated with a p56 tyrosine kinase to mediate T-cell activation signals through phosphorylation of adjacent proteins. Besides its important role in T-cell signalling, the CD8 co-receptor is also important for CD8+ T-cell development, stabilisation of the interface between CD8+ T-cells and target cells, and activation of intestinal epithelial T lymphocytes (iIELs). Therefore, CD8 has been the target of many therapeutic strategies designed to modulate T-cell responses during auto-immune reactions, allergy and organ transplant rejection.
Immunogen :
Synthetic peptide of mouse CD8a
Specificity :
Mouse CD8a
Purity :
Purified
Preservative :
0.05% NaN3
Concentration :
0.5 mg/ml
Application Summary :
IHC-P;other not tested
more info or order :
Dianova product webpage
company information
Dianova
Warburgstr. 45
D-20354 Hamburg
info@dianova.de
http://www.dianova.de
49 40 45 06 73 30
headquarters: Germany
Since its foundation in 1982, dianova GmbH provides professionals in Life Sciences with antibodies, immunoassays, and products for molecular biology. More than 30 years of grown expertise give rise to a fundamental knowledge about biochemical applications and a profound understanding of customer needs in research and routine diagnostic laboratories of universities, hospitals and the pharmaceutical industry.
Since its foundation in 1982, dianova GmbH provides professionals in Life Sciences with antibodies, immunoassays, and products for molecular biology. More than 30 years of grown expertise give rise to a fundamental knowledge about biochemical applications and a profound understanding of customer needs in research and routine diagnostic laboratories of universities, hospitals and the pharmaceutical industry.
In addition to distributing Life Science and Diagnostic Products in the German speaking region dianova manufactures and markets a series of antibodies with unique properties on a worldwide basis under the brand of optistain. To this end dianova has successfully established a distribution network in more than 25 countries.
At dianova quality is of paramount importance and subject to strict control. Internal controls and audits are carried out in addition to on-site inspections by independent, external inspection companies. In 2003 the TÜV Rheinland/Berlin/Brandenburg successfully completed the first comprehensive certification process. Since then dianova has been certified pursuant to the international DIN EN ISO 9001:2008 standard.