product summary
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company name :
Dianova
product type :
antibody
product name :
Anti-Fibroblasten (CD90) (Hu) from Mouse (Clone: AS02)
catalog :
DIA-100
quantity :
1 ml
price :
234 EUR plus VAT
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
AS02
reactivity :
human
application :
western blot, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - frozen section
more info or order :
Dianova product webpage
citations: 3
Reference
Saalbach A, Haustein U, Anderegg U. A ligand of human thy-1 is localized on polymorphonuclear leukocytes and monocytes and mediates the binding to activated thy-1-positive microvascular endothelial cells and fibroblasts. J Invest Dermatol. 2000;115:882-8 pubmed
Saalbach A, Wetzig T, Haustein U, Anderegg U. Detection of human soluble Thy-1 in serum by ELISA. Fibroblasts and activated endothelial cells are a possible source of soluble Thy-1 in serum. Cell Tissue Res. 1999;298:307-15 pubmed
Steiniger B, Barth P, Hellinger A. The perifollicular and marginal zones of the human splenic white pulp : do fibroblasts guide lymphocyte immigration?. Am J Pathol. 2001;159:501-12 pubmed
product information
Catalog Number :
DIA-100
Product Name :
Anti-Fibroblasten (CD90) (Hu) from Mouse (Clone: AS02)
Product Type :
Antibody
Host Species :
Mouse
Clonality :
Monoclonal
Conjugation :
nonconjugated
Clone Name :
AS02
Isotype :
IgG1
Light Chain :
kappa
Size :
1 ml
List Price :
234 EUR plus VAT
Product Description :
The monoclonal antibody clone AS02 reacts specifically with human CD90 (Thy-1), a GPI-anchored glycoprotein of the immunoglobulin superfamily with a molecular weight of 25 – 35 kD. In immunoblot analysis AS02 detects nonreduced CD90 at 30 kDa.
CrossReactivity :
No reaction with Rat (cultured fibroblasts, FC), Mouse (fibrosarcom cell line L929, FC), Rabbit (skin, IH), Pig (skin, IH), Monkey (skin, IH) Others not tested
Background :
CD90 (Thy-1) in human is primarily expressed by nerve cells, additionally in a sub-population (20%) of CD34+ blood stem cells and in various fibroblasts. In contrast to mouse and rat it is not expressed by thymocytes and peripheral blood T cells. AS02 recognizes fibroblasts of different origin but does not react with human blood cells, keratinocytes, resting micro-/macrovascular endothelial cells and components of the extracellular matrix like fibronectin, collagen type I, III, IV and laminin. Thus, AS02 is especially suitable for the specific detection and cell separation of human fibroblasts. Activated endothelial cells, after stimulation in vitro or in tissue sections of inflamed tissue in vivo, bind AS02 with different intensities, implying that CD90 (Thy-1) can be considered to be an activation marker of human endothelial cells. Recent papers demonstrate the binding of AS02 with activated microvascular endothelial cells, with a subpopulation of lung fibroblasts, specialized lymph node fibroblasts, but not lymphocytes and normal or inflamed synovial fibroblasts in human.
ALTnames :
CD90;Thy-1
Immunogen :
Human skin fibroblast
Specificity :
Human CD90 (Thy-1) (nerve cells, few CD34+ blood stem cells, fibroblasts)
Purity :
Purified
Preservative :
0.05% NaN3
Concentration :
0.2 mg/ml
Application Summary :
FACS;IHC-F;IF;IP;WB (nonreduced)
more info or order :
Dianova product webpage
company information
Dianova
Warburgstr. 45
D-20354 Hamburg
info@dianova.de
www.dianova.de
49 40 45 06 73 30
headquarters: Germany
Since its foundation in 1982, dianova GmbH provides professionals in Life Sciences with antibodies, immunoassays, and products for molecular biology. More than 30 years of grown expertise give rise to a fundamental knowledge about biochemical applications and a profound understanding of customer needs in research and routine diagnostic laboratories of universities, hospitals and the pharmaceutical industry.
Since its foundation in 1982, dianova GmbH provides professionals in Life Sciences with antibodies, immunoassays, and products for molecular biology. More than 30 years of grown expertise give rise to a fundamental knowledge about biochemical applications and a profound understanding of customer needs in research and routine diagnostic laboratories of universities, hospitals and the pharmaceutical industry.
In addition to distributing Life Science and Diagnostic Products in the German speaking region dianova manufactures and markets a series of antibodies with unique properties on a worldwide basis under the brand of optistain. To this end dianova has successfully established a distribution network in more than 25 countries.
At dianova quality is of paramount importance and subject to strict control. Internal controls and audits are carried out in addition to on-site inspections by independent, external inspection companies. In 2003 the TÜV Rheinland/Berlin/Brandenburg successfully completed the first comprehensive certification process. Since then dianova has been certified pursuant to the international DIN EN ISO 9001:2008 standard.