Synaptotagmin 2, synaptic marker | Developmental Studies Hybridoma Bank znp-1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Synaptotagmin 2, synaptic marker

catalog :

znp-1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

znp-1

reactivity :

human, mouse, rat, chicken, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 30

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; loading ...; fig s3g	Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, et al. Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition. Neuron. 2022;: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 1b3	Pelkey K, Calvigioni D, Fang C, Vargish G, Ekins T, Auville K, et al. Paradoxical network excitation by glutamate release from VGluT3+ GABAergic interneurons. elife. 2020;9: pubmed publisher
immunohistochemistry; zebrafish ; 1:100; loading ...; fig 2a	Oprişoreanu A, Smith H, Arya S, Webster R, Zhong Z, Wehner D, et al. Interaction of Axonal Chondrolectin with Collagen XIXa1 Is Necessary for Precise Neuromuscular Junction Formation. Cell Rep. 2019;29:1082-1098.e10 pubmed publisher
immunohistochemistry; mouse; 1:250; loading ...; fig 2c	Taetzsch T, Tenga M, Valdez G. Muscle Fibers Secrete FGFBP1 to Slow Degeneration of Neuromuscular Synapses during Aging and Progression of ALS. J Neurosci. 2017;37:70-82 pubmed publisher
immunohistochemistry; mouse; fig s6	Krishnaswamy A, Yamagata M, Duan X, Hong Y, Sanes J. Sidekick 2 directs formation of a retinal circuit that detects differential motion. Nature. 2015;524:466-470 pubmed publisher
immunohistochemistry; mouse; 1:100; fig 8	Adams K, Rousso D, Umbach J, Novitch B. Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells. Nat Commun. 2015;6:6778 pubmed publisher
immunohistochemistry; zebrafish ; 1:100; fig 2	Solchenberger B, Russell C, Kremmer E, Haass C, Schmid B. Granulin knock out zebrafish lack frontotemporal lobar degeneration and neuronal ceroid lipofuscinosis pathology. PLoS ONE. 2015;10:e0118956 pubmed publisher
	Zappa Villar M, López Hanotte J, Pardo J, Morel G, Mazzolini G, Garcia M, et al. Mesenchymal Stem Cells Therapy Improved the Streptozotocin-Induced Behavioral and Hippocampal Impairment in Rats. Mol Neurobiol. 2019;: pubmed publisher
	Chen C, Arai I, Satterfield R, Young S, Jonas P. Synaptotagmin 2 Is the Fast Ca2+ Sensor at a Central Inhibitory Synapse. Cell Rep. 2017;18:723-736 pubmed publisher
	Pei W, Xu L, Varshney G, Carrington B, Bishop K, Jones M, et al. Additive reductions in zebrafish PRPS1 activity result in a spectrum of deficiencies modeling several human PRPS1-associated diseases. Sci Rep. 2016;6:29946 pubmed publisher
	Li V, Tsui M, Chen X, Hui M, Jin L, Lam R, et al. Effects of 4-methylbenzylidene camphor (4-MBC) on neuronal and muscular development in zebrafish (Danio rerio) embryos. Environ Sci Pollut Res Int. 2016;23:8275-85 pubmed publisher
	Kotani Y, Morito D, Yamazaki S, Ogino K, Kawakami K, Takashima S, et al. Neuromuscular regulation in zebrafish by a large AAA+ ATPase/ubiquitin ligase, mysterin/RNF213. Sci Rep. 2015;5:16161 pubmed publisher
	Shan S, Boutin S, Ferdous J, Ali D. Ethanol exposure during gastrulation alters neuronal morphology and behavior in zebrafish. Neurotoxicol Teratol. 2015;48:18-27 pubmed publisher
	Wiley D, Juan I, Le H, Cai X, Baumbach L, BEATTIE C, et al. Yeast Augmented Network Analysis (YANA): a new systems approach to identify therapeutic targets for human genetic diseases. F1000Res. 2014;3:121 pubmed publisher
	Menelaou E, Udvadia A, Tanguay R, Svoboda K. Activation of ?2A-containing nicotinic acetylcholine receptors mediates nicotine-induced motor output in embryonic zebrafish. Eur J Neurosci. 2014;40:2225-40 pubmed publisher
	Valdez G, Heyer M, Feng G, Sanes J. The role of muscle microRNAs in repairing the neuromuscular junction. PLoS ONE. 2014;9:e93140 pubmed publisher
	Garcia Reyero N, Escalon B, Prats E, Stanley J, Thienpont B, Melby N, et al. Effects of BDE-209 contaminated sediments on zebrafish development and potential implications to human health. Environ Int. 2014;63:216-23 pubmed publisher
	Chen Z, Lee H, Henle S, Cheever T, Ekker S, Henley J. Primary neuron culture for nerve growth and axon guidance studies in zebrafish (Danio rerio). PLoS ONE. 2013;8:e57539 pubmed publisher
	Oksenberg N, Stevison L, Wall J, Ahituv N. Function and regulation of AUTS2, a gene implicated in autism and human evolution. PLoS Genet. 2013;9:e1003221 pubmed publisher
	Martin E, Schule R, Smets K, Rastetter A, Boukhris A, Loureiro J, et al. Loss of function of glucocerebrosidase GBA2 is responsible for motor neuron defects in hereditary spastic paraplegia. Am J Hum Genet. 2013;92:238-44 pubmed publisher
	Song Y, Wang M, Mao F, Shao M, Zhao B, Song Z, et al. Knockdown of Pnpla6 protein results in motor neuron defects in zebrafish. Dis Model Mech. 2013;6:404-13 pubmed publisher
	Brusegan C, Pistocchi A, Frassine A, Della Noce I, Schepis F, Cotelli F. Ccdc80-l1 Is involved in axon pathfinding of zebrafish motoneurons. PLoS ONE. 2012;7:e31851 pubmed publisher
	Lim A, Suli A, Yaniv K, Weinstein B, Li D, Chien C. Motoneurons are essential for vascular pathfinding. Development. 2011;138:3847-57 pubmed publisher
	Cooper A, Gillespie D. Synaptotagmins I and II in the developing rat auditory brainstem: Synaptotagmin I is transiently expressed in glutamate-releasing immature inhibitory terminals. J Comp Neurol. 2011;519:2417-33 pubmed publisher
	BRUSES J. N-cadherin regulates primary motor axon growth and branching during zebrafish embryonic development. J Comp Neurol. 2011;519:1797-815 pubmed publisher
	Fox M, Sanes J. Synaptotagmin I and II are present in distinct subsets of central synapses. J Comp Neurol. 2007;503:280-96 pubmed
	Hutchinson S, Eisen J. Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity. Development. 2006;133:2137-47 pubmed
	Hen Chow E, Cheng S. Cadmium affects muscle type development and axon growth in zebrafish embryonic somitogenesis. Toxicol Sci. 2003;73:149-59 pubmed
	Talbot W, Trevarrow B, Halpern M, Melby A, Farr G, Postlethwait J, et al. A homeobox gene essential for zebrafish notochord development. Nature. 1995;378:150-7 pubmed
	Trevarrow B, Marks D, Kimmel C. Organization of hindbrain segments in the zebrafish embryo. Neuron. 1990;4:669-79 pubmed

product information

Internal ID :

865

Name :

znp-1

Depositor Name :

Trevarrow, B.

Depositor Institution :

Institute of Neuroscience, University of Oregon

Depositor Website :

https://zfin.org/ZDB-PERS-960805-565

Date Deposited :

5/30/96

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

Synaptotagmin 2, synaptic marker

Antigen Species :

zebrafish

Host Species :

mouse

Isotype :

MIgG2a, kappa light chain

Isotype for catalog (legacy) :

IgG2a, kappa light chain

Positive Tested Species Reactivity :

Amphibian,Chicken,Human,Mouse,Rat,Zebrafish

Species Tested (legacy) :

Pos.: zebrafish embryos/larva, adults; betta; Neg.: Xenopus, trout, medaka, chick, shark, Haplochromis burtoni

Initial Publication Pubmed ID :

2344406

Depositor Notes (Special Instructions) :

Works well in zebrafish embryos/larva or adults. Stains (4% paraformaldehyde, 4ºC, O/N; cryostat or paraffin) the cytoplasm in neurons, early processes and neuropil regions.

Collections :

Human,Neurodevelopment

Search Keywords :

Trevarrow, B., Synaptotagmin 2 / Synapse marker, Zebrafish, MIgG2a, Kappa light chain, Zebrafish/Mouse/Amphibian/Rat/Human/Chicken, syt2b, , AB_2315626, Monoclonal, Neurodevelopment/Human, Immunohistochemistry/Immunofluorescence/Western blot/Immunoprecipitation/FFPE, znp1

Antigen Molecular Weight :

Expected: 48 kDa; Apparent: 60 kDa

Gene :

syt2a

Uniprot ID :

F1R0Q0

Antibody Registry ID :

AB_2315626

Immunogen :

Fixed embryos. Boosts: adult spinal cord

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP 2/0

Epitope Mapped :

Epitope Location or Sequence :

Although syt2 is heavily glycosylated, the znp-1 antibody recognizes a protein epitope.

Epitope Map PubMed ID :

17492637

Recommended Applications :

FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

2344406 7477317 12700413 21456023

Immunofluorescence Pubmed IDs :

16672347 27425195 23332916 17492637 25599605 24317228 26888529 25785851

Western Blot Pubmed IDs :

17492637 24738729 31399955 31399955

Immunoprecipitation Pubmed IDs :

17492637

FFPE Pubmed IDs :

2344406

Additional Information :

Recognizes primary motor neurones.

DSHB Growth Medium :

Iscove's

References (legacy) :

Neuron 4, 669-679.; "The Zebrafish Book", Univ. of Oregon Press; edition 2.1.; Development 133, 2137-2147.

more info or order :

Developmental Studies Hybridoma Bank product webpage