product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
CD166 antigen homolog A (Neurolin)
catalog :
zn-8
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ZN-8
reactivity :
zebrafish
application :
immunohistochemistry, immunocytochemistry
citations: 16
Reference
Shimizu H, Langenbacher A, Huang J, Wang K, Otto G, Geisler R, et al. The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes. elife. 2017;6: pubmed publisher
Noack Watt K, Achilleos A, Neben C, Merrill A, Trainor P. The Roles of RNA Polymerase I and III Subunits Polr1c and Polr1d in Craniofacial Development and in Zebrafish Models of Treacher Collins Syndrome. PLoS Genet. 2016;12:e1006187 pubmed publisher
Malicki J, Pooranachandran N, Nikolaev A, Fang X, Avanesov A. Analysis of the retina in the zebrafish model. Methods Cell Biol. 2016;134:257-334 pubmed publisher
Shan S, Boutin S, Ferdous J, Ali D. Ethanol exposure during gastrulation alters neuronal morphology and behavior in zebrafish. Neurotoxicol Teratol. 2015;48:18-27 pubmed publisher
Garcia Reyero N, Escalon B, Prats E, Stanley J, Thienpont B, Melby N, et al. Effects of BDE-209 contaminated sediments on zebrafish development and potential implications to human health. Environ Int. 2014;63:216-23 pubmed publisher
Yuelling L, Waggener C, Afshari F, Lister J, Fuss B. Autotaxin/ENPP2 regulates oligodendrocyte differentiation in vivo in the developing zebrafish hindbrain. Glia. 2012;60:1605-18 pubmed publisher
Otten C, van der Ven P, Lewrenz I, Paul S, Steinhagen A, Busch Nentwich E, et al. Xirp proteins mark injured skeletal muscle in zebrafish. PLoS ONE. 2012;7:e31041 pubmed publisher
Kirchmaier B, Poon K, Schwerte T, Huisken J, Winkler C, Jungblut B, et al. The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development. Dev Biol. 2012;363:438-50 pubmed publisher
Kim H, Dorsky R. Tcf7l1 is required for spinal cord progenitor maintenance. Dev Dyn. 2011;240:2256-64 pubmed publisher
Johnson C, Hernandez Lagunas L, Feng W, Melvin V, Williams T, Artinger K. Vgll2a is required for neural crest cell survival during zebrafish craniofacial development. Dev Biol. 2011;357:269-81 pubmed publisher
Volkmann K, Chen Y, Harris M, Wullimann M, Köster R. The zebrafish cerebellar upper rhombic lip generates tegmental hindbrain nuclei by long-distance migration in an evolutionary conserved manner. J Comp Neurol. 2010;518:2794-817 pubmed publisher
Riley B, Sweet E, Heck R, Evans A, McFarland K, Warga R, et al. Characterization of harpy/Rca1/emi1 mutants: patterning in the absence of cell division. Dev Dyn. 2010;239:828-43 pubmed publisher
Paridaen J, Danesin C, Elas A, van de Water S, Houart C, Zivkovic D. Apc1-mediated antagonism of Wnt/beta-catenin signaling is required for retino-tectal pathfinding in the zebrafish. Zebrafish. 2009;6:41-7 pubmed publisher
Menelaou E, Svoboda K. Secondary motoneurons in juvenile and adult zebrafish: axonal pathfinding errors caused by embryonic nicotine exposure. J Comp Neurol. 2009;512:305-22 pubmed publisher
Kawahara A, Chien C, Dawid I. The homeobox gene mbx is involved in eye and tectum development. Dev Biol. 2002;248:107-17 pubmed
Trevarrow B, Marks D, Kimmel C. Organization of hindbrain segments in the zebrafish embryo. Neuron. 1990;4:669-79 pubmed
product information
Internal ID :
860
Name :
ZN-8
Depositor Name :
Trevarrow, B.
Depositor Institution :
Institute of Neuroscience, University of Oregon
Date Deposited :
5/30/96
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
CD166 antigen homolog A (Neurolin)
Antigen Species :
zebrafish
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
zebrafish
Species Tested (legacy) :
Pos.: zebrafish embryos/larva, adults; Neg.: Xenopus (st36), betta (1d, 2d), chick, shark, Haplochromis burtoni
Initial Publication Pubmed ID :
2344406
Depositor Notes (Special Instructions) :
Doesn't work on Xenopus (st36), betta fish (1d, 2d), chicken, shark and Haplochromis burtoni. Works on zebrafish embryos/larva and adults. Stains (4% paraformaldehyde, 4ºC, O/N, cryostat or wholemount) the cell surface on commissural fibers, secondary Motoneurons, outer surface of somites, some ganglia, radial glial cells and the anterior commissure.
Collections :
CD antigens,Cell markers,Neurodevelopment
Search Keywords :
Trevarrow, neuronal cell surface marker (SC-1, DM-GRASP, BEN), zebrafish, mouse, alcam, zn5, zn-5, AB_531904, IgG1, Immunofluorescence,Immunohistochemistry
Antigen Molecular Weight :
Predicted: 59 kDa
Gene :
alcama
Alternate Gene Name(s) :
alcam, cd166, DM-GRASP
Uniprot ID :
Q90460
Antibody Registry ID :
AB_531904
Immunogen :
Zebrafish embryo membranes (2 days old)
Immunogen Sequence :
Full length protein
Myeloma Strain :
SP 2/0
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry
Immunoblotting (legacy) :
see Monte Westerfield
Immunohistochemistry Pubmed IDs :
2344406 20146251 22821873
Immunofluorescence Pubmed IDs :
12142024 22355335 22290329 21932308 21741961 19292673 27312496 27448281 25599605 24317228 28826496 31351039
Additional Information :
zn-5 and zn-8 are two clones from the same hybridoma. Recognizes secondary motor neurones.
DSHB Growth Medium :
Iscove's
References (legacy) :
Neuron 4, 669-679, The Zebrafish Book, Univ. of Oregon Press; edition 2.1.; Dev. Biol. 248, 107-117.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US