product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
CD166 antigen homolog A (Neurolin)
catalog :
zn-8
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ZN-8
reactivity :
zebrafish
application :
immunohistochemistry, immunocytochemistry
more info or order :
citations: 16
product information
Internal ID :
860
Name :
ZN-8
Depositor Name :
Trevarrow, B.
Depositor Institution :
Institute of Neuroscience, University of Oregon
Date Deposited :
5/30/96
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
CD166 antigen homolog A (Neurolin)
Antigen Species :
zebrafish
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
zebrafish
Species Tested (legacy) :
Pos.: zebrafish embryos/larva, adults; Neg.: Xenopus (st36), betta (1d, 2d), chick, shark, Haplochromis burtoni
Initial Publication Pubmed ID :
2344406
Depositor Notes (Special Instructions) :
Doesn't work on Xenopus (st36), betta fish (1d, 2d), chicken, shark and Haplochromis burtoni. Works on zebrafish embryos/larva and adults. Stains (4% paraformaldehyde, 4ºC, O/N, cryostat or wholemount) the cell surface on commissural fibers, secondary Motoneurons, outer surface of somites, some ganglia, radial glial cells and the anterior commissure.
Collections :
CD antigens,Cell markers,Neurodevelopment
Search Keywords :
Trevarrow, neuronal cell surface marker (SC-1, DM-GRASP, BEN), zebrafish, mouse, alcam, zn5, zn-5, AB_531904, IgG1, Immunofluorescence,Immunohistochemistry
Antigen Molecular Weight :
Predicted: 59 kDa
Gene :
alcama
Alternate Gene Name(s) :
alcam, cd166, DM-GRASP
Uniprot ID :
Q90460
Antibody Registry ID :
AB_531904
Immunogen :
Zebrafish embryo membranes (2 days old)
Immunogen Sequence :
Full length protein
Myeloma Strain :
SP 2/0
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry
Immunoblotting (legacy) :
see Monte Westerfield
Immunohistochemistry Pubmed IDs :
2344406 20146251 22821873
Immunofluorescence Pubmed IDs :
12142024 22355335 22290329 21932308 21741961 19292673 27312496 27448281 25599605 24317228 28826496 31351039
Additional Information :
zn-5 and zn-8 are two clones from the same hybridoma. Recognizes secondary motor neurones.
DSHB Growth Medium :
Iscove's
References (legacy) :
Neuron 4, 669-679, The Zebrafish Book, Univ. of Oregon Press; edition 2.1.; Dev. Biol. 248, 107-117.
more info or order :
company information
Developmental Studies Hybridoma Bank
product type
browse more products
questions and comments