product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
neuronal cell surface marker (HNK-1 carbohydrate epitope)
catalog :
zn-12
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
zn-12
reactivity :
zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 22
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; zebrafish ; 1:250; loading ...; fig 10c
Haynes E, Burnett K, He J, Jean Pierre M, Jarzyna M, Eliceiri K, et al. KLC4 shapes axon arbors during development and mediates adult behavior. elife. 2022;11: pubmed publisher
Nelson H, Coffing G, Chilson S, Hester K, Carrillo C, Ostreicher S, et al. Structure, development, and functional morphology of the cement gland of the giant danio, Devario malabaricus. Dev Dyn. 2019;248:1155-1174 pubmed publisher
SHEETS L. Excessive activation of ionotropic glutamate receptors induces apoptotic hair-cell death independent of afferent and efferent innervation. Sci Rep. 2017;7:41102 pubmed publisher
Zachar P, Pan W, Jonz M. Distribution and morphology of cholinergic cells in the branchial epithelium of zebrafish (Danio rerio). Cell Tissue Res. 2017;367:169-179 pubmed publisher
Dean B, Rashid T, Jonz M. Mitogenic action of hypoxia upon cutaneous neuroepithelial cells in developing zebrafish. Dev Neurobiol. 2017;77:789-801 pubmed publisher
Buckland Nicks J. New details of the neural architecture of the salmonid adipose fin. J Fish Biol. 2016;89:1991-2003 pubmed publisher
Ma L, Shen H, Shen Y, Schachner M. The Adhesion Molecule-Characteristic HNK-1 Carbohydrate Contributes to Functional Recovery After Spinal Cord Injury in Adult Zebrafish. Mol Neurobiol. 2017;54:3253-3263 pubmed publisher
Mundell N, BEIER K, Pan Y, Lapan S, Göz Aytürk D, Berezovskii V, et al. Vesicular stomatitis virus enables gene transfer and transsynaptic tracing in a wide range of organisms. J Comp Neurol. 2015;523:1639-63 pubmed publisher
Ogun O, Zallocchi M. Clarin-1 acts as a modulator of mechanotransduction activity and presynaptic ribbon assembly. J Cell Biol. 2014;207:375-91 pubmed publisher
Moreno R, Ribera A. Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability. Neural Dev. 2014;9:19 pubmed publisher
Menelaou E, Udvadia A, Tanguay R, Svoboda K. Activation of ?2A-containing nicotinic acetylcholine receptors mediates nicotine-induced motor output in embryonic zebrafish. Eur J Neurosci. 2014;40:2225-40 pubmed publisher
Newton C, Stoyek M, Croll R, Smith F. Regional innervation of the heart in the goldfish, Carassius auratus: a confocal microscopy study. J Comp Neurol. 2014;522:456-78 pubmed publisher
Ruiz Perera L, Arruti C, Zolessi F. Early phosphorylation of MARCKS at Ser25 in migrating precursor cells and differentiating peripheral neurons. Neurosci Lett. 2013;544:5-9 pubmed publisher
Lewellis S, Nagelberg D, Subedi A, Staton A, LeBlanc M, GIRALDEZ A, et al. Precise SDF1-mediated cell guidance is achieved through ligand clearance and microRNA-mediated decay. J Cell Biol. 2013;200:337-55 pubmed publisher
Nourizadeh Lillabadi R, Seilø Torgersen J, Vestrheim O, König M, Alestrom P, Syed M. Early embryonic gene expression profiling of zebrafish prion protein (Prp2) morphants. PLoS ONE. 2010;5:e13573 pubmed publisher
den Broeder M, van der Linde H, Brouwer J, Oostra B, Willemsen R, Ketting R. Generation and characterization of FMR1 knockout zebrafish. PLoS ONE. 2009;4:e7910 pubmed publisher
Bayliss P, Bellavance K, Whitehead G, Abrams J, Aegerter S, Robbins H, et al. Chemical modulation of receptor signaling inhibits regenerative angiogenesis in adult zebrafish. Nat Chem Biol. 2006;2:265-73 pubmed
Lacosta A, Muniesa P, Ruberte J, Sarasa M, Dominguez L. Novel expression patterns of Pax3/Pax7 in early trunk neural crest and its melanocyte and non-melanocyte lineages in amniote embryos. Pigment Cell Res. 2005;18:243-51 pubmed
Ataian Y, Owens J, Hinterberger T. MRF4 gene expression in Xenopus embryos and aneural myofibers. Dev Dyn. 2003;226:551-4 pubmed
Hatta K, Puschel A, Kimmel C. Midline signaling in the primordium of the zebrafish anterior central nervous system. Proc Natl Acad Sci U S A. 1994;91:2061-5 pubmed
Trevarrow B, Marks D, Kimmel C. Organization of hindbrain segments in the zebrafish embryo. Neuron. 1990;4:669-79 pubmed
Metcalfe W, Myers P, Trevarrow B, Bass M, Kimmel C. Primary neurons that express the L2/HNK-1 carbohydrate during early development in the zebrafish. Development. 1990;110:491-504 pubmed
product information
Internal ID :
855
Name :
zn-12
Depositor Name :
Trevarrow, B.
Depositor Institution :
Institute of Neuroscience, University of Oregon
Date Deposited :
5/30/96
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
neuronal cell surface marker (HNK-1 carbohydrate epitope)
Antigen Species :
zebrafish
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Chicken,Fish,Shark,Xenopus,Zebrafish
Species Tested (legacy) :
Pos.: zebrafish embryos/larva, adults, betta, Xenopus, shark, chick, Haplochromis burtoni
Initial Publication Pubmed ID :
2344406
Depositor Notes (Special Instructions) :
Works on zebrafish embryos/larva and adults. Stains (4% paraformaldehyde, 4ºC, O/N, cryostat or wholemount) the cell surface of many but not all neurons, fibers and neuropil, muscle cells and receptor cells.
Collections :
Cell markers,Cell signaling,Neurodevelopment,Xenopus
Search Keywords :
Trevarrow, neuronal cell surface marker, zebrafish, mouse, zn12, HNK-1, AB_531908, IgG1, chicken, fish, shark, xenopus, Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot, monoclonal, HNK1
Antibody Registry ID :
AB_531908
Immunogen :
Adult brain membranes. Boosts: adult CNS membranes
Clonality :
Monoclonal
Myeloma Strain :
SP 2/0
Epitope Mapped :
Yes
Epitope Location or Sequence :
HNK-1 carbohydrate epitope
Epitope Map PubMed ID :
1723944
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
2344406 1723944 12619139 16029418 7907792 31310039
Immunofluorescence Pubmed IDs :
16565716 7907792 27909858 27501745 27907267 23853005 23470634 23853005 25688551 28112265
Western Blot Pubmed IDs :
1723944 24738729 27086029
Immunoprecipitation Pubmed IDs :
1723944
Additional Information :
Zn-12 recognizes both sulfated and non-sulfated HNK-1 epitopes.
Additional Characterization :
https://zfin.org/zf_info/zfbook/zfmabs.html
DSHB Growth Medium :
Iscove's
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US