singed (fascin) | Developmental Studies Hybridoma Bank sn 7C product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

singed (fascin)

catalog :

sn 7C

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

sn 7C

reactivity :

fruit fly

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:25; fig 1a western blot; fruit fly; 1:20; fig 4f	Groen C, Jayo A, Parsons M, Tootle T. Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture. Mol Biol Cell. 2015;26:1901-17 pubmed publisher
	Otani T, Ogura Y, Misaki K, Maeda T, Kimpara A, Yonemura S, et al. IKK? inhibits PKC to promote Fascin-dependent actin bundling. Development. 2016;143:3806-3816 pubmed
	Aranjuez G, Burtscher A, Sawant K, Majumder P, McDonald J. Dynamic myosin activation promotes collective morphology and migration by locally balancing oppositional forces from surrounding tissue. Mol Biol Cell. 2016;27:1898-910 pubmed publisher
	Cai D, Chen S, Prasad M, He L, Wang X, Choesmel Cadamuro V, et al. Mechanical feedback through E-cadherin promotes direction sensing during collective cell migration. Cell. 2014;157:1146-59 pubmed publisher
	Martorell O, Merlos Suarez A, Campbell K, Barriga F, Christov C, Miguel Aliaga I, et al. Conserved mechanisms of tumorigenesis in the Drosophila adult midgut. PLoS ONE. 2014;9:e88413 pubmed publisher
	Aranjuez G, Kudlaty E, Longworth M, McDonald J. On the role of PDZ domain-encoding genes in Drosophila border cell migration. G3 (Bethesda). 2012;2:1379-91 pubmed publisher
	Groen C, Spracklen A, Fagan T, Tootle T. Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling. Mol Biol Cell. 2012;23:4567-78 pubmed publisher
	Zanet J, Jayo A, Plaza S, Millard T, Parsons M, Stramer B. Fascin promotes filopodia formation independent of its role in actin bundling. J Cell Biol. 2012;197:477-86 pubmed publisher
	Khammari A, Agnes F, Gandille P, Pret A. Physiological apoptosis of polar cells during Drosophila oogenesis is mediated by Hid-dependent regulation of Diap1. Cell Death Differ. 2011;18:793-805 pubmed publisher
	Evans I, Hu N, Skaer H, Wood W. Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos. Development. 2010;137:1625-33 pubmed publisher
	Defaye A, Evans I, Crozatier M, Wood W, Lemaitre B, Leulier F. Genetic ablation of Drosophila phagocytes reveals their contribution to both development and resistance to bacterial infection. J Innate Immun. 2009;1:322-34 pubmed publisher
	Zanet J, Payre F, Plaza S. Fascin for cell migration in Drosophila. Fly (Austin). 2009;3:281-2 pubmed
	Jang A, Chang Y, Bai J, Montell D. Border-cell migration requires integration of spatial and temporal signals by the BTB protein Abrupt. Nat Cell Biol. 2009;11:569-79 pubmed publisher
	Wang X, Adam J, Montell D. Spatially localized Kuzbanian required for specific activation of Notch during border cell migration. Dev Biol. 2007;301:532-40 pubmed
	Wang X, Bo J, Bridges T, Dugan K, Pan T, Chodosh L, et al. Analysis of cell migration using whole-genome expression profiling of migratory cells in the Drosophila ovary. Dev Cell. 2006;10:483-95 pubmed
	Silver D, Geisbrecht E, Montell D. Requirement for JAK/STAT signaling throughout border cell migration in Drosophila. Development. 2005;132:3483-92 pubmed
	McDonald J, Montell D. Analysis of cell migration using Drosophila as a model system. Methods Mol Biol. 2005;294:175-202 pubmed
	Pinheiro E, Montell D. Requirement for Par-6 and Bazooka in Drosophila border cell migration. Development. 2004;131:5243-51 pubmed
	Rogers S, Wiedemann U, Stuurman N, Vale R. Molecular requirements for actin-based lamella formation in Drosophila S2 cells. J Cell Biol. 2003;162:1079-88 pubmed
	Cant K, Knowles B, Mahajan Miklos S, Heintzelman M, Cooley L. Drosophila fascin mutants are rescued by overexpression of the villin-like protein, quail. J Cell Sci. 1998;111 ( Pt 2):213-21 pubmed
	Cant K, Cooley L. Single amino acid mutations in Drosophila fascin disrupt actin bundling function in vivo. Genetics. 1996;143:249-58 pubmed
	Cant K, Knowles B, Mooseker M, Cooley L. Drosophila singed, a fascin homolog, is required for actin bundle formation during oogenesis and bristle extension. J Cell Biol. 1994;125:369-80 pubmed
	Pai L, Batra J, Fitzgerald D, Willingham M, Pastan I. Antitumor effects of B3-PE and B3-LysPE40 in a nude mouse model of human breast cancer and the evaluation of B3-PE toxicity in monkeys. Cancer Res. 1992;52:3189-93 pubmed

product information

Internal ID :

1837

Name :

sn 7C

Depositor Name :

Cooley, L.

Depositor Institution :

Yale Medical School

Date Deposited :

7/12/01

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

singed (fascin)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

8163553

Depositor Notes (Special Instructions) :

Use 1:20 in Blotto-Tween (PBS, 5% dry milk, 0.2% Tween) for Westerns or undiluted for ovary staining. (Cant et al., 1994). This antibody doesn't appear to work for immunoprecipitation or purification.

Collections :

Cell adhesion,Cytoskeleton,Drosophila antigens

Search Keywords :

Cooley, L., singed (fascin), Drosophila, MIgG1, kappa light chain, Drosophila, sn, CG1536, AB_528239, monoclonal, Drosophila antigens/Cytoskeletal elements/Cell adhesion, Immunofluorescence/Western Blot/ELISA

Antigen Molecular Weight :

Predicted: 57 kDa; Apparent: 57 kDa

Gene :

Alternate Gene Name(s) :

CG1536

Uniprot ID :

Q24524

Antibody Registry ID :

AB_528239

Immunogen :

Recombinant GST-singed fusion protein

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Epitope Mapped :

Recommended Applications :

ELISA,Immunofluorescence,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

8163553 20392742 1591729 15576913 27122602 24855950 16000386 17010965 15456726 23173089 16580993 21113144 24516653 19350016 8722779

Western Blot Pubmed IDs :

8163553 23051736 22564415 12975351 19901519 8722779 9405306

ELISA Pubmed IDs :

8163553

Pubmed IDs :

20375589

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 125, 369-380.; J. Innate Immun. 1(4), 322-334.Development 137(10), 1625-1633.; doi: 10.1091/mbc.E12-05-0417

more info or order :

Developmental Studies Hybridoma Bank product webpage