cadherin, E- (canine) | Developmental Studies Hybridoma Bank rr1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

cadherin, E- (canine)

catalog :

rr1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

rr1

reactivity :

dog

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; dog; 1:500; loading ...; fig 2f	Rangel L, Bernabé Rubio M, Fernández Barrera J, Casares Arias J, Millan J, Alonso M, et al. Caveolin-1α regulates primary cilium length by controlling RhoA GTPase activity. Sci Rep. 2019;9:1116 pubmed publisher
western blot; dog; 1:100; loading ...; fig 3a	Grieve A, Rabouille C. Extracellular cleavage of E-cadherin promotes epithelial cell extrusion. J Cell Sci. 2014;127:3331-46 pubmed publisher
	Yu Kemp H, Kemp J, Brieher W. CRMP-1 enhances EVL-mediated actin elongation to build lamellipodia and the actin cortex. J Cell Biol. 2017;216:2463-2479 pubmed publisher
	Gloerich M, Bianchini J, Siemers K, Cohen D, Nelson W. Cell division orientation is coupled to cell-cell adhesion by the E-cadherin/LGN complex. Nat Commun. 2017;8:13996 pubmed publisher
	Capra J, Eskelinen S. MDCK cells are capable of water secretion and reabsorption in response to changes in the ionic environment. Can J Physiol Pharmacol. 2017;95:72-83 pubmed publisher
	Yu H, Dohn M, Markham N, Coffey R, Reynolds A. p120-catenin controls contractility along the vertical axis of epithelial lateral membranes. J Cell Sci. 2016;129:80-94 pubmed publisher
	Cerda O, CÃ¡ceres M, Park K, Leiva Salcedo E, Romero A, Varela D, et al. Casein kinase-mediated phosphorylation of serine 839 is necessary for basolateral localization of the CaÂ²âº-activated non-selective cation channel TRPM4. Pflugers Arch. 2015;467:1723-1732 pubmed publisher
	Lubkov V, Bar Sagi D. E-cadherin-mediated cell coupling is required for apoptotic cell extrusion. Curr Biol. 2014;24:868-74 pubmed publisher
	Andersen M, Krzystanek K, Petersen F, Bomholtz S, Olesen S, Abriel H, et al. A phosphoinositide 3-kinase (PI3K)-serum- and glucocorticoid-inducible kinase 1 (SGK1) pathway promotes Kv7.1 channel surface expression by inhibiting Nedd4-2 protein. J Biol Chem. 2013;288:36841-54 pubmed publisher
	Bush K, Tsukamoto T, Nigam S. Selective degradation of E-cadherin and dissolution of E-cadherin-catenin complexes in epithelial ischemia. Am J Physiol Renal Physiol. 2000;278:F847-52 pubmed
	Gumbiner B, Simons K. A functional assay for proteins involved in establishing an epithelial occluding barrier: identification of a uvomorulin-like polypeptide. J Cell Biol. 1986;102:457-68 pubmed

product information

Internal ID :

3819

Name :

rr1

Depositor Name :

Gumbiner, B.M.

Depositor Institution :

Cell Biology, School of Medicine, University of Virginia

Date Deposited :

5/7/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

cadherin, E- (canine)

Antigen Species :

canine

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Canine

Species Tested (legacy) :

canine only

Initial Publication Pubmed ID :

3511070

Depositor Notes (Special Instructions) :

rr1 is canine specific. Blocks epithelial cell-cell adhesion and cell junction formation. Stains all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.

Collections :

CD antigens,Cell adhesion

Search Keywords :

Gumbiner, B.M., cadherin, E- (canine), canine, MIgG1, canine, CDH1, uvo, CD324, AB_528114, monoclonal, Cell adhesion/Cluster Determinant antigens, Immunofluorescence/Western Blot/Immunoprecipitation/Function Blocking

Antigen Molecular Weight :

Predicted: 80 kDa (mature E-cadherin); Apparent 118 kDa

Gene :

CDH1

Alternate Gene Name(s) :

uvo, CD324

Uniprot ID :

F1PAA9

Antibody Registry ID :

AB_528114

Immunogen :

Madin Darby Canine Kidney (MDCK) cell line, whole cells

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

Ag8

Epitope Mapped :

Epitope Location or Sequence :

Extracellular domain 81 kDa tryptic fragment

Epitope Map PubMed ID :

3511070

Recommended Applications :

Function Blocking,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

3511070 24704076 28045117 24895403 26585313 25231975 27901358 24214981

Western Blot Pubmed IDs :

3511070 10807598 24704076 24895403

Immunoprecipitation Pubmed IDs :

3511070 10807598

Function Blocking Pubmed IDs :

3511070

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell Biol. 102, 457-468.; Am. J. Physiol. Renal Physiol. 278, F847-F852.

more info or order :

Developmental Studies Hybridoma Bank product webpage