rough Drosophila protein | Developmental Studies Hybridoma Bank ro-62C2A8 anti-rough product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

rough Drosophila protein

catalog :

ro-62C2A8 anti-rough

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ro-62C2A8 anti-rough

reactivity :

fruit fly

application :

immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 16

Reference
Avet Rochex A, Carvajal N, Christoforou C, Yeung K, Maierbrugger K, Hobbs C, et al. Unkempt is negatively regulated by mTOR and uncouples neuronal differentiation from growth control. PLoS Genet. 2014;10:e1004624 pubmed publisher
Kang J, Yeom E, Lim J, Choi K. Bar represses dPax2 and decapentaplegic to regulate cell fate and morphogenetic cell death in Drosophila eye. PLoS ONE. 2014;9:e88171 pubmed publisher
Giagtzoglou N, Li T, Yamamoto S, Bellen H. Drosophila EHBP1 regulates Scabrous secretion during Notch-mediated lateral inhibition. J Cell Sci. 2013;126:3686-96 pubmed publisher
Nicolay B, Bayarmagnai B, Moon N, Benevolenskaya E, Frolov M. Combined inactivation of pRB and hippo pathways induces dedifferentiation in the Drosophila retina. PLoS Genet. 2010;6:e1000918 pubmed publisher
O Keefe D, Gonzalez Niño E, Burnett M, Dylla L, Lambeth S, Licon E, et al. Rap1 maintains adhesion between cells to affect Egfr signaling and planar cell polarity in Drosophila. Dev Biol. 2009;333:143-60 pubmed publisher
Almudi I, Stocker H, Hafen E, Corominas M, Serras F. SOCS36E specifically interferes with Sevenless signaling during Drosophila eye development. Dev Biol. 2009;326:212-23 pubmed publisher
McNeill H, Craig G, Bateman J. Regulation of neurogenesis and epidermal growth factor receptor signaling by the insulin receptor/target of rapamycin pathway in Drosophila. Genetics. 2008;179:843-53 pubmed publisher
Lim J, Lee O, Hsu Y, Singh A, Choi K. Drosophila TRAP230/240 are essential coactivators for Atonal in retinal neurogenesis. Dev Biol. 2007;308:322-30 pubmed
Sukhanova M, Deb D, Gordon G, Matakatsu M, Du W. Proneural basic helix-loop-helix proteins and epidermal growth factor receptor signaling coordinately regulate cell type specification and cdk inhibitor expression during development. Mol Cell Biol. 2007;27:2987-96 pubmed
Voas M, Rebay I. The novel plant homeodomain protein rhinoceros antagonizes Ras signaling in the Drosophila eye. Genetics. 2003;165:1993-2006 pubmed
Sun Y, Kanekar S, Vetter M, Gorski S, Jan Y, Glaser T, et al. Conserved and divergent functions of Drosophila atonal, amphibian, and mammalian Ath5 genes. Evol Dev. 2003;5:532-41 pubmed
Kaminker J, Canon J, Salecker I, Banerjee U. Control of photoreceptor axon target choice by transcriptional repression of Runt. Nat Neurosci. 2002;5:746-50 pubmed
Das G, Reynolds Kenneally J, Mlodzik M. The atypical cadherin Flamingo links Frizzled and Notch signaling in planar polarity establishment in the Drosophila eye. Dev Cell. 2002;2:655-66 pubmed
Nagel A, Preiss A. Notchspl is deficient for inductive processes in the eye, and E(spl)D enhances split by interfering with proneural activity. Dev Biol. 1999;208:406-15 pubmed
Dominguez M, Wasserman J, Freeman M. Multiple functions of the EGF receptor in Drosophila eye development. Curr Biol. 1998;8:1039-48 pubmed
Kimmel B, Heberlein U, Rubin G. The homeo domain protein rough is expressed in a subset of cells in the developing Drosophila eye where it can specify photoreceptor cell subtype. Genes Dev. 1990;4:712-27 pubmed

product information

Internal ID :

1921

Name :

ro-62C2A8 anti-rough

Depositor Name :

Rubin, G.M.

Depositor Institution :

University of California at Berkeley

Date Deposited :

8/31/94

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

rough Drosophila protein

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Initial Publication Pubmed ID :

1974228

Depositor Notes (Special Instructions) :

The protein rough is present in the nucleus of most or all of the cells in the morphogenetic furrow of the larval eye, and is limited to the R2, R3, R4, and R5 cells in the region posterior to the furrow. Antibody purified by ammonium salt precipitation may be more effective than supernatant. Use PEMP as a fixative.

Collections :

Cell signaling,Drosophila antigens,Transcription factors

Search Keywords :

Rubin, G.M., rough Drosophila protein, Drosophila, MIgG1, kappa light chain, Drosophila, ro, CG6348, AB_528456, monoclonal, Drosophila antigens/Transcription factors/Cell signaling, Immunohistochemistry/Immunofluorescence

Antigen Molecular Weight :

Predicted: 39 kDa

Gene :

Alternate Gene Name(s) :

CG6348

Uniprot ID :

P10181

Antibody Registry ID :

AB_528456

Synonyms (Alternate Clone Names) :

mAbro1

Immunogen :

Recmbinant fusion protein between protein A and aa 182-238 of Drosophila rough

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3x63Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 182-238

Epitope Map PubMed ID :

1974228

Recommended Applications :

Immunofluorescence,Immunohistochemistry

Immunohistochemistry Pubmed IDs :

1974228

Immunofluorescence Pubmed IDs :

10191054 17296729 14704181 23788431 18505882 17585897 19083999 19576205 25210733 20421993 24505414 12015972 12118258 9768358 12950631

DSHB Growth Medium :

Iscove's

References (legacy) :

Genes Dev. 4, 712-727.; Dev. Biol. 208, 406-415.

more info or order :

Developmental Studies Hybridoma Bank product webpage