p53 | Developmental Studies Hybridoma Bank p53 7A4 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

p53

catalog :

p53 7A4

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

p53 7A4

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 5

Reference
Chakraborty R, Li Y, Zhou L, Golic K. Corp Regulates P53 in Drosophila melanogaster via a Negative Feedback Loop. PLoS Genet. 2015;11:e1005400 pubmed publisher
Nakamura M, Ohsawa S, Igaki T. Mitochondrial defects trigger proliferation of neighbouring cells via a senescence-associated secretory phenotype in Drosophila. Nat Commun. 2014;5:5264 pubmed publisher
Simón R, Aparicio R, Housden B, Bray S, Busturia A. Drosophila p53 controls Notch expression and balances apoptosis and proliferation. Apoptosis. 2014;19:1430-43 pubmed publisher
Herzog G, Joerger A, Shmueli M, Fersht A, Gazit E, Segal D. Evaluating Drosophila p53 as a model system for studying cancer mutations. J Biol Chem. 2012;287:44330-7 pubmed publisher
Brodsky M, Weinert B, Tsang G, Rong Y, McGinnis N, Golic K, et al. Drosophila melanogaster MNK/Chk2 and p53 regulate multiple DNA repair and apoptotic pathways following DNA damage. Mol Cell Biol. 2004;24:1219-31 pubmed

product information

Internal ID :

1909

Name :

p53 7A4

Depositor Name :

Rubin, G.M.

Depositor Institution :

University of California at Berkeley

Date Deposited :

8/2/06

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

p53

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, lambda light chain

Isotype for catalog (legacy) :

IgG1, lambda light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

14729967

Depositor Notes (Special Instructions) :

This antibody has been succesfully used for Immunoprecipitation.

Collections :

Cell signaling,Drosophila antigens,Transcription factors

Search Keywords :

Rubin, G.M., p53, Drosophila, MIgG1, lambda light chain, Drosophila, p53, CG33336, prac, AB_579786, monoclonal, Transcription factors/Drosophila antigens/Cell signaling, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Multiple isoforms of different MW; Apparent 50 kDa

Gene :

p53

Alternate Gene Name(s) :

CG33336, prac

Uniprot ID :

Q9N6D8

Antibody Registry ID :

AB_579786

Immunogen :

Recombinant GST fusion protein of Drosophila p53 (aa 282-386 [Uniprot ID Q9N6D8]; common to all isoforms)

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3XAg8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 282-386

Epitope Map PubMed ID :

14729967

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

24858703 26230084 25345385

Western Blot Pubmed IDs :

23135266

Additional Information :

This antibody recognizes the C-terminal region common to all Drosophila p53 isoforms. p53 is the homolog of the Cellular tumor antigen p53.

DSHB Growth Medium :

Iscove's

References (legacy) :

Mol. Cell. Biol. 24, 1219-1231.; Cell 101, 103-113.

more info or order :

Developmental Studies Hybridoma Bank product webpage