Sir2 (Drosophila; Sirtuin 1) | Developmental Studies Hybridoma Bank p4A10 (anti-dSir2) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Sir2 (Drosophila; Sirtuin 1)

catalog :

p4A10 (anti-dSir2)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

p4A10 (anti-dSir2)

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, western blot knockout validation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; fruit fly; 1:50; fig 4f	Palu R, Thummel C. Sir2 Acts through Hepatocyte Nuclear Factor 4 to maintain insulin Signaling and Metabolic Homeostasis in Drosophila. PLoS Genet. 2016;12:e1005978 pubmed publisher
	Pek J, Ng B, Kai T. Polo-mediated phosphorylation of Maelstrom regulates oocyte determination during oogenesis in Drosophila. Development. 2012;139:4505-13 pubmed publisher

product information

Internal ID :

5747

Name :

p4A10 (anti-dSir2)

Depositor Name :

Parkhurst, S.

Depositor Institution :

Fred Hutchinson Cancer Research Center

Date Deposited :

12/9/08

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Sir2 (Drosophila; Sirtuin 1)

Antigen Species :

Drosophila melanogaster

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila (other species not tested)

Initial Publication Pubmed ID :

23136393

Collections :

Drosophila antigens,Enzymes,Nucleus

Search Keywords :

Parkhurst, S., Sir2 (Drosophila; Sirtuin 1), Drosophila melanogaster, MIgG1, Drosophila, Sirt1, CG5216, Sir2, AB_1553778, monoclonal, Drosophila antigens/Nucleus/Enzymes, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 92 kDa (FlyBase FBgn0024291); Apparent: 110 kDa

Gene :

Sirt1

Alternate Gene Name(s) :

CG5216, Sir2

Uniprot ID :

Q86LE9

Antibody Registry ID :

AB_1553778

Immunogen :

Recombinant GST fusion protein of Drosophila Sir2

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

23136393

Western Blot Pubmed IDs :

27058248

Additional Information :

Drosophila Sir2 belongs to the sirtuin family.

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 109, 447-458.

more info or order :

Developmental Studies Hybridoma Bank product webpage