product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
rho1
catalog :
p1D9 (anti-rho1)
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
p1D9 (anti-rho1)
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 16
Published Application/Species/Sample/DilutionReference
  • western blot; fruit fly; 1:10
Koppen T, Weckmann A, Muller S, Staubach S, Bloch W, Dohmen R, et al. Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells. Proteomics. 2011;11:4397-410 pubmed publisher
LeBlanc M, Lehmann R. Domain-specific control of germ cell polarity and migration by multifunction Tre1 GPCR. J Cell Biol. 2017;216:2945-2958 pubmed publisher
Mason F, Xie S, Vasquez C, Tworoger M, Martin A. RhoA GTPase inhibition organizes contraction during epithelial morphogenesis. J Cell Biol. 2016;214:603-17 pubmed publisher
Das A, Shah S, Fan B, Paik D, DiSanto D, Hinman A, et al. Spindle Assembly and Chromosome Segregation Requires Central Spindle Proteins in Drosophila Oocytes. Genetics. 2016;202:61-75 pubmed publisher
Verboon J, Rahe T, Rodriguez Mesa E, Parkhurst S. Wash functions downstream of Rho1 GTPase in a subset of Drosophila immune cell developmental migrations. Mol Biol Cell. 2015;26:1665-74 pubmed publisher
Eliazer S, Palacios V, Wang Z, Kollipara R, Kittler R, Buszczak M. Lsd1 restricts the number of germline stem cells by regulating multiple targets in escort cells. PLoS Genet. 2014;10:e1004200 pubmed publisher
Toret C, D Ambrosio M, Vale R, Simon M, Nelson W. A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion. J Cell Biol. 2014;204:265-79 pubmed publisher
Uehara R, Goshima G, Mabuchi I, Vale R, Spudich J, Griffis E. Determinants of myosin II cortical localization during cytokinesis. Curr Biol. 2010;20:1080-5 pubmed publisher
Castoreno A, Smurnyy Y, Torres A, Vokes M, Jones T, Carpenter A, et al. Small molecules discovered in a pathway screen target the Rho pathway in cytokinesis. Nat Chem Biol. 2010;6:457-63 pubmed publisher
Neisch A, Speck O, Stronach B, Fehon R. Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. J Cell Biol. 2010;189:311-23 pubmed publisher
Patch K, Stewart S, Welch A, Ward R. A second-site noncomplementation screen for modifiers of Rho1 signaling during imaginal disc morphogenesis in Drosophila. PLoS ONE. 2009;4:e7574 pubmed publisher
Simoes S, Denholm B, Azevedo D, Sotillos S, Martin P, Skaer H, et al. Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis. Development. 2006;133:4257-67 pubmed
Elwell C, Engel J. Drosophila melanogaster S2 cells: a model system to study Chlamydia interaction with host cells. Cell Microbiol. 2005;7:725-39 pubmed
Magie C, Parkhurst S. Rho1 regulates signaling events required for proper Drosophila embryonic development. Dev Biol. 2005;278:144-54 pubmed
Rogers S, Wiedemann U, Hacker U, Turck C, Vale R. Drosophila RhoGEF2 associates with microtubule plus ends in an EB1-dependent manner. Curr Biol. 2004;14:1827-33 pubmed
Magie C, Pinto Santini D, Parkhurst S. Rho1 interacts with p120ctn and alpha-catenin, and regulates cadherin-based adherens junction components in Drosophila. Development. 2002;129:3771-82 pubmed
product information
Internal ID :
3939
Name :
p1D9 (anti-rho1)
Depositor Name :
Parkhurst, S.
Depositor Institution :
Fred Hutchinson Cancer Research Center
Depositor Website :
http://research.fhcrc.org/parkhurst/en.html
Date Deposited :
4/9/02
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
rho1
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
12135916
Depositor Notes (Special Instructions) :
This antigen is also known as Ras-like GTP-binding protein Rho1.
Collections :
Cell adhesion,Cell signaling,Cytoskeleton,Drosophila antigens
Search Keywords :
Parkhurst, S. , rho1, Drosophila, MIgG1, Drosophila, Rho1, , AB_528263, Monoclonal, Drosophila antigens/Cell Signaling, Immunohistochemistry/Immunofluorescence/Western blot/Immunoprecipitation
Antigen Molecular Weight :
21.7 kDa
Gene :
Rho1
Uniprot ID :
A4UZI6
Antibody Registry ID :
AB_528263
Immunogen :
Rho 1 cDNA/GST fusion protein
Immunogen Sequence :
Full length sequence
Clonality :
Monoclonal
Myeloma Strain :
p1D9-A8
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
17021037
Immunofluorescence Pubmed IDs :
12135916 15498490 20404112 15649467 15839901 26564158 27551058 20541410 28687666 20436488 24625679
Western Blot Pubmed IDs :
12135916 25739458 19862331 24446484
Immunoprecipitation Pubmed IDs :
12135916
Additional Information :
RRID:AB_528263 FBgn0014020
DSHB Growth Medium :
RPMI
References (legacy) :
Development 129, 3771-3782.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US