product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
orb protein
catalog :
orb 6H4
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
orb 6H4
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 17
Reference
Costa A, Pazman C, Sinsimer K, Wong L, McLeod I, Yates J, et al. Rasputin functions as a positive regulator of orb in Drosophila oogenesis. PLoS ONE. 2013;8:e72864 pubmed publisher
Yan R, McKee B. The cohesion protein SOLO associates with SMC1 and is required for synapsis, recombination, homolog bias and cohesion and pairing of centromeres in Drosophila Meiosis. PLoS Genet. 2013;9:e1003637 pubmed publisher
Xu S, Hafer N, Agunwamba B, Schedl P. The CPEB protein Orb2 has multiple functions during spermatogenesis in Drosophila melanogaster. PLoS Genet. 2012;8:e1003079 pubmed publisher
Rojas Ríos P, Guerrero I, González Reyes A. Cytoneme-mediated delivery of hedgehog regulates the expression of bone morphogenetic proteins to maintain germline stem cells in Drosophila. PLoS Biol. 2012;10:e1001298 pubmed publisher
Azzam G, Smibert P, Lai E, Liu J. Drosophila Argonaute 1 and its miRNA biogenesis partners are required for oocyte formation and germline cell division. Dev Biol. 2012;365:384-94 pubmed publisher
Wong L, Schedl P. Cup blocks the precocious activation of the orb autoregulatory loop. PLoS ONE. 2011;6:e28261 pubmed publisher
Pepper A, Beerman R, Bhogal B, Jongens T. Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. PLoS ONE. 2009;4:e7618 pubmed publisher
Benoit P, Papin C, Kwak J, Wickens M, Simonelig M. PAP- and GLD-2-type poly(A) polymerases are required sequentially in cytoplasmic polyadenylation and oogenesis in Drosophila. Development. 2008;135:1969-79 pubmed publisher
Bogard N, Lan L, Xu J, Cohen R. Rab11 maintains connections between germline stem cells and niche cells in the Drosophila ovary. Development. 2007;134:3413-8 pubmed
Benoit B, Mitou G, Chartier A, Temme C, Zaessinger S, Wahle E, et al. An essential cytoplasmic function for the nuclear poly(A) binding protein, PABP2, in poly(A) tail length control and early development in Drosophila. Dev Cell. 2005;9:511-22 pubmed
Costa A, Wang Y, Dockendorff T, Erdjument Bromage H, Tempst P, Schedl P, et al. The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway. Dev Cell. 2005;8:331-42 pubmed
Navarro C, Puthalakath H, Adams J, Strasser A, Lehmann R. Egalitarian binds dynein light chain to establish oocyte polarity and maintain oocyte fate. Nat Cell Biol. 2004;6:427-35 pubmed
Manheim E, McKim K. The Synaptonemal complex component C(2)M regulates meiotic crossing over in Drosophila. Curr Biol. 2003;13:276-85 pubmed
Martin S, St Johnston D. A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity. Nature. 2003;421:379-84 pubmed
Mansfield J, Wilhelm J, Hazelrigg T. Ypsilon Schachtel, a Drosophila Y-box protein, acts antagonistically to Orb in the oskar mRNA localization and translation pathway. Development. 2002;129:197-209 pubmed
Jordan K, Clegg N, Blasi J, Morimoto A, Sen J, Stein D, et al. The homeobox gene mirror links EGF signalling to embryonic dorso-ventral axis formation through notch activation. Nat Genet. 2000;24:429-33 pubmed
Lantz V, Chang J, Horabin J, Bopp D, Schedl P. The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity. Genes Dev. 1994;8:598-613 pubmed
product information
Internal ID :
2066
Name :
orb 6H4
Depositor Name :
Schedl, P.
Depositor Institution :
Princeton University
Date Deposited :
3/16/99
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
orb protein
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG2a, lambda light chain
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
7523244
Collections :
Drosophila antigens
Search Keywords :
Schedl, P., orb protein, Drosophila, MIgG2a, lambda light chain, Drosophila, orb, CG10868, AB_528419, monoclonal, Drosophila antigens, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation
Antigen Molecular Weight :
Multiple isoforms; major bands have an apparent MW between 97 and 100 kDa
Gene :
orb
Alternate Gene Name(s) :
CG10868
Uniprot ID :
Q9VCN7
Antibody Registry ID :
AB_528419
Immunogen :
Recombinant fusion protein of phage T7 gene Phi10 and oo18 RNA-binding protein (aa 94-765 of 915 aa isoform [Uniprot ID Q9VCN7])
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Myeloma Strain :
Sp2/0
Epitope Mapped :
Yes
Epitope Location or Sequence :
aa 94-765 of 915 aa isoform [Uniprot ID Q9VCN7], containing the rrm (RNA recognition motif) regions
Epitope Map PubMed ID :
7523244
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunofluorescence Pubmed IDs :
23209437 7523244 12540903 11782413 15737929 22164257 24069162 17715175 10742112 15077115 12593793 22509132
Western Blot Pubmed IDs :
23209437 7523244 11782413 15737929 22164257 24069162 18434412 16198293
Immunoprecipitation Pubmed IDs :
15737929 22164257 24069162 18434412 16198293
Additional Information :
orb is a RNA binding protein
DSHB Growth Medium :
Iscove's
References (legacy) :
Genes Dev. 8, 598-613.; Dev. Biol. 365, 384-394.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US