product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
orb protein
catalog :
orb 4H8
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
orb 4H8
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 17
Reference
Ryazansky S, Kotov A, Kibanov M, Akulenko N, Korbut A, Lavrov S, et al. RNA helicase Spn-E is required to maintain Aub and AGO3 protein levels for piRNA silencing in the germline of Drosophila. Eur J Cell Biol. 2016;95:311-22 pubmed publisher
Davidson A, Parton R, Rabouille C, Weil T, Davis I. Localized Translation of gurken/TGF-α mRNA during Axis Specification Is Controlled by Access to Orb/CPEB on Processing Bodies. Cell Rep. 2016;14:2451-62 pubmed publisher
Ghosh S, Lasko P. Loss-of-function analysis reveals distinct requirements of the translation initiation factors eIF4E, eIF4E-3, eIF4G and eIF4G2 in Drosophila spermatogenesis. PLoS ONE. 2015;10:e0122519 pubmed publisher
Mukai M, Hira S, Nakamura K, Nakamura S, Kimura H, Sato M, et al. H3K36 Trimethylation-Mediated Epigenetic Regulation is Activated by Bam and Promotes Germ Cell Differentiation During Early Oogenesis in Drosophila. Biol Open. 2015;4:119-24 pubmed publisher
Costa A, Pazman C, Sinsimer K, Wong L, McLeod I, Yates J, et al. Rasputin functions as a positive regulator of orb in Drosophila oogenesis. PLoS ONE. 2013;8:e72864 pubmed publisher
Xu S, Hafer N, Agunwamba B, Schedl P. The CPEB protein Orb2 has multiple functions during spermatogenesis in Drosophila melanogaster. PLoS Genet. 2012;8:e1003079 pubmed publisher
Rojas Ríos P, Guerrero I, González Reyes A. Cytoneme-mediated delivery of hedgehog regulates the expression of bone morphogenetic proteins to maintain germline stem cells in Drosophila. PLoS Biol. 2012;10:e1001298 pubmed publisher
Ji Y, Tulin A. Poly(ADP-ribose) controls DE-cadherin-dependent stem cell maintenance and oocyte localization. Nat Commun. 2012;3:760 pubmed publisher
Wong L, Schedl P. Cup blocks the precocious activation of the orb autoregulatory loop. PLoS ONE. 2011;6:e28261 pubmed publisher
Kim S, Gailite I, Moussian B, Luschnig S, Goette M, Fricke K, et al. Kinase-activity-independent functions of atypical protein kinase C in Drosophila. J Cell Sci. 2009;122:3759-71 pubmed publisher
Koch C, Honemann Capito M, Egger Adam D, Wodarz A. Windei, the Drosophila homolog of mAM/MCAF1, is an essential cofactor of the H3K9 methyl transferase dSETDB1/Eggless in germ line development. PLoS Genet. 2009;5:e1000644 pubmed publisher
Neumüller R, Betschinger J, Fischer A, Bushati N, Poernbacher I, Mechtler K, et al. Mei-P26 regulates microRNAs and cell growth in the Drosophila ovarian stem cell lineage. Nature. 2008;454:241-5 pubmed publisher
Chicoine J, Benoit P, Gamberi C, Paliouras M, Simonelig M, Lasko P. Bicaudal-C recruits CCR4-NOT deadenylase to target mRNAs and regulates oogenesis, cytoskeletal organization, and its own expression. Dev Cell. 2007;13:691-704 pubmed
Penn J, Schedl P. The master switch gene sex-lethal promotes female development by negatively regulating the N-signaling pathway. Dev Cell. 2007;12:275-86 pubmed
Manheim E, McKim K. The Synaptonemal complex component C(2)M regulates meiotic crossing over in Drosophila. Curr Biol. 2003;13:276-85 pubmed
Martin S, St Johnston D. A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity. Nature. 2003;421:379-84 pubmed
Lantz V, Chang J, Horabin J, Bopp D, Schedl P. The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity. Genes Dev. 1994;8:598-613 pubmed
product information
Internal ID :
2060
Name :
orb 4H8
Depositor Name :
Schedl, P.
Depositor Institution :
Princeton University
Date Deposited :
3/16/99
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
orb protein
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, lambda light chain
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
7523244
Collections :
Drosophila antigens
Search Keywords :
Schedl, P., orb protein, Drosophila, MIgG1, lambda light chain, Drosophila, orb, CG10868, AB_528418, monoclonal, Drosophila antigens, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation
Antigen Molecular Weight :
Multiple isoforms; major bands have an apparent MW between 97 and 100 kDa
Gene :
orb
Alternate Gene Name(s) :
CG10868
Uniprot ID :
Q9VCN7
Antibody Registry ID :
AB_528418
Immunogen :
Recombinant fusion protein of phage T7 gene Phi10 and Oo18 RNA-binding protein (aa 94-765 of 915 aa isoform [Uniprot ID Q9VCN7])
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Myeloma Strain :
Sp2/0
Epitope Mapped :
Yes
Epitope Location or Sequence :
aa 94-765 of 915 aa isoform [Uniprot ID Q9VCN7], containing the rrm (RNA recognition motif) regions
Epitope Map PubMed ID :
7523244
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunofluorescence Pubmed IDs :
23209437 7523244 12540903 22164257 12593793 22509132 18528333 22453833 25572421 17276344 17981137 19789180 19750210 25849588 31292144
Western Blot Pubmed IDs :
23209437 27320195 7523244 22164257 24069162
Immunoprecipitation Pubmed IDs :
22164257 24069162
Additional Information :
orb is a RNA binding protein
DSHB Growth Medium :
Iscove's
References (legacy) :
Genes Dev. 8, 598-613.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US