neuroglian (Manduca sexta) | Developmental Studies Hybridoma Bank neuroglian 3B11 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

neuroglian (Manduca sexta)

catalog :

neuroglian 3B11

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

neuroglian 3B11

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Reference
Gibson N, Tolbert L, Oland L. Roles of specific membrane lipid domains in EGF receptor activation and cell adhesion molecule stabilization in a developing olfactory system. PLoS ONE. 2009;4:e7222 pubmed publisher
Coate T, Swanson T, Copenhaver P. Reverse signaling by glycosylphosphatidylinositol-linked Manduca ephrin requires a SRC family kinase to restrict neuronal migration in vivo. J Neurosci. 2009;29:3404-18 pubmed publisher
Zhuang S, Kelo L, Nardi J, Kanost M. Multiple alpha subunits of integrin are involved in cell-mediated responses of the Manduca immune system. Dev Comp Immunol. 2008;32:365-79 pubmed
Zhuang S, Kelo L, Nardi J, Kanost M. Neuroglian on hemocyte surfaces is involved in homophilic and heterophilic interactions of the innate immune system of Manduca sexta. Dev Comp Immunol. 2007;31:1159-67 pubmed
Gibson N, Tolbert L. Activation of epidermal growth factor receptor mediates receptor axon sorting and extension in the developing olfactory system of the moth Manduca sexta. J Comp Neurol. 2006;495:554-72 pubmed
Nardi J, Pilas B, Bee C, Zhuang S, Garsha K, Kanost M. Neuroglian-positive plasmatocytes of Manduca sexta and the initiation of hemocyte attachment to foreign surfaces. Dev Comp Immunol. 2006;30:447-62 pubmed
Nardi J, Zhuang S, Pilas B, Bee C, Kanost M. Clustering of adhesion receptors following exposure of insect blood cells to foreign surfaces. J Insect Physiol. 2005;51:555-64 pubmed
Nardi J, Pilas B, Ujhelyi E, Garsha K, Kanost M. Hematopoietic organs of Manduca sexta and hemocyte lineages. Dev Genes Evol. 2003;213:477-91 pubmed
Chen C, Lampe D, Robertson H, Nardi J. Neuroglian is expressed on cells destined to form the prothoracic glands of Manduca embryos as they segregate from surrounding cells and rearrange during morphogenesis. Dev Biol. 1997;181:1-13 pubmed
Nardi J. Rearrangement of epithelial cell types in an insect wing monolayer is accompanied by differential expression of a cell surface protein. Dev Dyn. 1994;199:315-25 pubmed

product information

Internal ID :

6991

Name :

neuroglian 3B11

Depositor Name :

Nardi, J.

Depositor Institution :

Department of Entomology, University of Illinois

Date Deposited :

1/3/11

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

neuroglian (Manduca sexta)

Antigen Species :

Manduca sexta

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Manduca sexta

Species Tested (legacy) :

Manduca sexta

Initial Publication Pubmed ID :

8075433

Collections :

Cell adhesion,Cell markers,Neurodevelopment

Search Keywords :

Nardi, J., neuroglian (Manduca sexta), Manduca sexta, MIgG1, Manduca sexta, Nrg, , AB_10804674, Monoclonal, Cell markers/Neurodevelopment/cell adhesion, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/Function Blocking

Antigen Molecular Weight :

Predicted: 140 kDa; Apparent: 156 kDa

Gene :

Nrg

Uniprot ID :

P91767

Antibody Registry ID :

AB_10804674

Immunogen :

Developing adult (four days after pupation) wing epithelia membrane preparartion.

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 18-605 (extracellular domain encompassing the six Ig domains)

Epitope Map PubMed ID :

16171863

Recommended Applications :

Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

8075433 9015260 14551769

Immunofluorescence Pubmed IDs :

16171863 16498681 19787046 15894002 19295147

Western Blot Pubmed IDs :

16171863 17448535 16498681 19787046

Immunoprecipitation Pubmed IDs :

8075433

Function Blocking Pubmed IDs :

17448535

Pubmed IDs :

17868866

Additional Information :

Recognizes the extracellular region (encompassing the six Ig domains) of neuroglian. Works on western blots under nonreducing conditions. The initial publication for this antibody is: Nardi, J.B. (1993). Modulated expression of a surface epitope on migrating germ cells of Manduca sexta embryos. Development 118, 967-975.

DSHB Growth Medium :

Iscove's

References (legacy) :

Development 118, 967-975.; Dev. Dyn. 199, 315-325.; Dev. Biol. 181, 1-13.; Dev. Comp. Immunol. 30, 447-462.; Dev. Comp. Immunol. 31, 1159-1167.

more info or order :

Developmental Studies Hybridoma Bank product webpage