F-actin capping protein alpha-1 & alpha-2 subunits | Developmental Studies Hybridoma Bank mAb 5B12.3 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

F-actin capping protein alpha-1 & alpha-2 subunits

catalog :

mAb 5B12.3

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

mAb 5B12.3

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

product information

Internal ID :

1855

Name :

mAb 5B12.3

Depositor Name :

Cooper, J.A.

Depositor Institution :

Washington University Med. School, St. Louis

Depositor Website :

https://cooperlab.wustl.edu/

Date Deposited :

2/13/96

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

F-actin capping protein alpha-1 & alpha-2 subunits

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG2a, kappa light chain

Isotype for catalog (legacy) :

IgG2a, kappa light chain

Positive Tested Species Reactivity :

Chicken,Human,Mouse,Rat

Species Tested (legacy) :

chicken, mouse, human

Initial Publication Pubmed ID :

8858171

Depositor Notes (Special Instructions) :

For immunofluorescence staining, fixation with a glyoxal-based solution has been successful (PMID: 24319057/ 28899994) while paraformaldehyde has generally not succeeded. Glyoxal solutions can be prepared and used as described (PMID: 29146773) and are commercially available under the name GlyoFixx.

Collections :

Cytoskeleton,Human,Neurodevelopment,Skeletal muscle

Search Keywords :

Cooper, J.A., F-actin capping protein alpha-1 & alpha-2 subunits, Chicken, M IgG2a, kappa light chain, Chicken/Human/Mouse/Rat, CAPZA1, CAPPA1, CAPZ, CAZ1, , Monoclonal, Cytoskeleton/Skeletal muscle/Human/Neurodevelopment, Immunoprecipitation/Western blot/Immunohistochemistry/Immunofluorescence

Antigen Molecular Weight :

32.9 kDa

Gene :

CAPZA1

Alternate Gene Name(s) :

CAPPA1, CAPZ, CAZ1

Uniprot ID :

P13127

Immunogen :

Recombinant GST-capping protein-alpha

Immunogen Sequence :

Full length

Clonality :

Monoclonal

Myeloma Strain :

P3x63 Ag8.653

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

28899994

Immunofluorescence Pubmed IDs :

21752999 24319057

Western Blot Pubmed IDs :

8858171 9484958 9331217 21752999 14990563 16987810 22918941 24319057

Immunoprecipitation Pubmed IDs :

28899994

Function Blocking Pubmed IDs :

8858171 12690097

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 135(1), 169-179.

more info or order :

Developmental Studies Hybridoma Bank product webpage