B-cadherin (cadherin-3) | Developmental Studies Hybridoma Bank mAb 5A6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

B-cadherin (cadherin-3)

catalog :

mAb 5A6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

mAb 5A6

reactivity :

chicken

application :

ELISA, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 6

Reference
Duguay D, Foty R, Steinberg M. Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants. Dev Biol. 2003;253:309-23 pubmed
Yamada H, Fredette B, Shitara K, Hagihara K, Miura R, Ranscht B, et al. The brain chondroitin sulfate proteoglycan brevican associates with astrocytes ensheathing cerebellar glomeruli and inhibits neurite outgrowth from granule neurons. J Neurosci. 1997;17:7784-95 pubmed
Tugizov S, Maidji E, Pereira L. Role of apical and basolateral membranes in replication of human cytomegalovirus in polarized retinal pigment epithelial cells. J Gen Virol. 1996;77 ( Pt 1):61-74 pubmed
Murphy Erdosh C, Napolitano E, Reichardt L. The expression of B-cadherin during embryonic chick development. Dev Biol. 1994;161:107-25 pubmed
Murphy Erdosh C, Yoshida C, Paradies N, Reichardt L. The cadherin-binding specificities of B-cadherin and LCAM. J Cell Biol. 1995;129:1379-90 pubmed
Redies C, Muller H. Similarities in structure and expression between mouse P-cadherin, chicken B-cadherin and frog XB/U-cadherin. Cell Adhes Commun. 1994;2:511-20 pubmed

product information

Internal ID :

1499

Name :

mAb 5A6

Depositor Name :

Reichardt, L.

Depositor Institution :

Department of Physiology, University of California

Date Deposited :

4/20/99

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

B-cadherin (cadherin-3)

Antigen Species :

chicken

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Chicken

Species Tested (legacy) :

chick

Initial Publication Pubmed ID :

8293867

Depositor Notes (Special Instructions) :

mAb 5A6 doesn't work on SDS-denatured B-cadherin. It recognizes the extracellular domain.

Collections :

Cardiac development,Cell adhesion,Cell signaling,Cytoskeleton,Neurodevelopment

Search Keywords :

Reichardt, L., B-cadherin (cadherin-3), chicken, MIgG1, chicken, K-CAM, CDH3, AB_528112, monoclonal, Cell adhesion/Neurodevelopment/Cytoskeletal elements/Cell signaling/Cardiac development, Immunofluorescence/Immunoprecipitation/ELISA/Function Blocking

Antigen Molecular Weight :

Predicted: 80 kDa; Apparent: 120 kDa

Gene :

K-CAM

Alternate Gene Name(s) :

CDH3

Uniprot ID :

P33145

Antibody Registry ID :

AB_528112

Synonyms (Alternate Clone Names) :

5A6

Immunogen :

L929 mouse cells expressing recombinant chick B-cadherin

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

extracellular domain (see depositor notes)

Recommended Applications :

ELISA,Function Blocking,Immunofluorescence,Immunoprecipitation

Immunoblotting (legacy) :

Immunofluorescence Pubmed IDs :

8293867 7775581 12645933 8558129

Immunoprecipitation Pubmed IDs :

8293867

ELISA Pubmed IDs :

8293867

Function Blocking Pubmed IDs :

7775581 12645933

Pubmed IDs :

9315899 7743137

Additional Information :

B-cadherin is also known as cadherin 3.

DSHB Growth Medium :

Iscove's

References (legacy) :

Dev. Biol. 161,107-125; J. Cell Biol. 129, 1379-1390

more info or order :

Developmental Studies Hybridoma Bank product webpage