synaptotagmin-1, cytoplasmic domain | Developmental Studies Hybridoma Bank mAb 48 (asv 48) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

synaptotagmin-1, cytoplasmic domain

catalog :

mAb 48 (asv 48)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

mAb 48 (asv 48)

reactivity :

human, mouse, rat, chicken

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Reference
Fogerson S, van Brummen A, Busch D, Allen S, Roychaudhuri R, Banks S, et al. Reducing synuclein accumulation improves neuronal survival after spinal cord injury. Exp Neurol. 2016;278:105-15 pubmed publisher
Taguchi K, Watanabe Y, Tsujimura A, Tanaka M. Brain region-dependent differential expression of alpha-synuclein. J Comp Neurol. 2016;524:1236-58 pubmed publisher
Moritz C, Eckstein E, Tenzer S, Friauf E. Neuroproteomics in the auditory brainstem: candidate proteins for ultrafast and precise information processing. Mol Cell Neurosci. 2015;64:9-23 pubmed publisher
Dagley L, White C, Liao Y, Shi W, Smyth G, Orian J, et al. Quantitative proteomic profiling reveals novel region-specific markers in the adult mouse brain. Proteomics. 2014;14:241-61 pubmed publisher
Lau B, Fogerson S, Walsh R, Morgan J. Cyclic AMP promotes axon regeneration, lesion repair and neuronal survival in lampreys after spinal cord injury. Exp Neurol. 2013;250:31-42 pubmed publisher
Casas C, Herrando Grabulosa M, Manzano R, Mancuso R, Osta R, Navarro X. Early presymptomatic cholinergic dysfunction in a murine model of amyotrophic lateral sclerosis. Brain Behav. 2013;3:145-58 pubmed publisher
Cooper A, Gillespie D. Synaptotagmins I and II in the developing rat auditory brainstem: Synaptotagmin I is transiently expressed in glutamate-releasing immature inhibitory terminals. J Comp Neurol. 2011;519:2417-33 pubmed publisher
Fox M, Sanes J. Synaptotagmin I and II are present in distinct subsets of central synapses. J Comp Neurol. 2007;503:280-96 pubmed
Matthew W, Tsavaler L, Reichardt L. Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue. J Cell Biol. 1981;91:257-69 pubmed
Greif K, Reichardt L. Appearance and distribution of neuronal cell surface and synaptic vesicle antigens in the developing rat superior cervical ganglion. J Neurosci. 1982;2:843-52 pubmed

product information

Internal ID :

1493

Name :

mAb 48 (asv 48)

Depositor Name :

Reichardt, L.

Depositor Institution :

Department of Physiology, University of California

Date Deposited :

4/20/99

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

synaptotagmin-1, cytoplasmic domain

Antigen Species :

rat

Host Species :

mouse

Isotype :

MIgG2b, kappa light chain

Isotype for catalog (legacy) :

IgG2b, kappa light chain

Positive Tested Species Reactivity :

Chicken,Fish,Human,Lamprey,Mouse,Rat,Xenopus,Zebrafish

Species Tested (legacy) :

fish, Xenopus, chick, mouse, rat, Zebrafish and human

Initial Publication Pubmed ID :

7298720

Depositor Notes (Special Instructions) :

Not available to for-profit organizations. For further information please contact the Office of Innovation, Technology, and Alliances at the University of California. Refer to case number 95-082. Email : ita@ucsf.edu Telephone: 415 502-1603. Researchers at non-profit institutions must sign an additional compliance form prior to purchasing this antibody. The compliance form is available by emailing dshb@uiowa.edu or by calling 319 335-3826.

Collections :

Cell markers,Human,Neurodevelopment

Search Keywords :

Reichardt, synaptotagmin 1, cytoplasmic domain, Syt1, P65, monoclonal, AB_2199314, IgG2b, Chicken,Fish,Human,Lamprey,Mouse,Rat,Xenopus,Zebrafish, ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Antigen Molecular Weight :

Predicted: 47 kDa; Apparent: 65 kDa

Gene :

Syt1

Alternate Gene Name(s) :

P65

Uniprot ID :

P21707

Antibody Registry ID :

AB_2199314

Synonyms (Alternate Clone Names) :

SV 48

Immunogen :

Synaptic junctional complex proteins prepared from rat brain synaptic membranes

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

Cytoplasmic (aa 80-421)

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes, detergent sensitive

Immunohistochemistry Pubmed IDs :

7298720 6212651 21456023

Immunofluorescence Pubmed IDs :

17492637 21456023 24041988 26854933 25131618 23531559 26358191

Western Blot Pubmed IDs :

7298720 17492637 24041988 24259518 24041988 31399955 31399955

Immunoprecipitation Pubmed IDs :

17492637

Additional Information :

This antibody was validated for tissue fixed with 4% paraformaldehyde and cryosections following OCT embedding. Detergent sensitive / Triton X-100. This antibody recognizes synaptotagmin 1 and does not cross react with synaptotagmin 2. See Fox and Sanes (2007, PUDMED 17492637). mAb 48 (asv 48) has a higher affinity than mAb 30 (asv30). The two antibodies recognize the same antigen, Syt1.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 81, 257-269; J. Neurosci. 2, 843-852.; J. Comp. Neurol. 503, 280-296.; J. Comp. Neurol. 519, 2417-2433.

more info or order :

Developmental Studies Hybridoma Bank product webpage