acetylcholine nicotinic receptors, muscle | Developmental Studies Hybridoma Bank mAb 35 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

acetylcholine nicotinic receptors, muscle

catalog :

mAb 35

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

mAb 35

application :

immunohistochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Reference
Oprişoreanu A, Smith H, Arya S, Webster R, Zhong Z, Wehner D, et al. Interaction of Axonal Chondrolectin with Collagen XIXa1 Is Necessary for Precise Neuromuscular Junction Formation. Cell Rep. 2019;29:1082-1098.e10 pubmed publisher
Schmidt N, Akaaboune M, Gajendran N, Martinez Pena y Valenzuela I, Wakefield S, Thurnheer R, et al. Neuregulin/ErbB regulate neuromuscular junction development by phosphorylation of ?-dystrobrevin. J Cell Biol. 2011;195:1171-84 pubmed publisher
Cui W, Saint Amant L, Kuwada J. shocked Gene is required for the function of a premotor network in the zebrafish CNS. J Neurophysiol. 2004;92:2898-908 pubmed
Begum S, Komiyama M, Toyota N, Obinata T, Maruyama K, Shimada Y. Differentiation of muscle-specific proteins in chicken somites as studied by immunofluorescence microscopy. Cell Tissue Res. 1998;293:305-11 pubmed
Tzartos S, Seybold M, Lindstrom J. Specificities of antibodies to acetylcholine receptors in sera from myasthenia gravis patients measured by monoclonal antibodies. Proc Natl Acad Sci U S A. 1982;79:188-92 pubmed
Tzartos S, Rand D, Einarson B, Lindstrom J. Mapping of surface structures of electrophorus acetylcholine receptor using monoclonal antibodies. J Biol Chem. 1981;256:8635-45 pubmed
Whiting P, Liu R, Morley B, Lindstrom J. Structurally different neuronal nicotinic acetylcholine receptor subtypes purified and characterized using monoclonal antibodies. J Neurosci. 1987;7:4005-16 pubmed
Kubalek E, Ralston S, Lindstrom J, Unwin N. Location of subunits within the acetylcholine receptor by electron image analysis of tubular crystals from Torpedo marmorata. J Cell Biol. 1987;105:9-18 pubmed
Watson J, Adkins Regan E, Whiting P, Lindstrom J, Podleski T. Autoradiographic localization of nicotinic acetylcholine receptors in the brain of the zebra finch (Poephila guttata). J Comp Neurol. 1988;274:255-64 pubmed
Berg D, Boyd R, Halvorsen S, Higgins L, Jacob M, Margiotta J. Regulating the number and function of neuronal acetylcholine receptors. Trends Neurosci. 1989;12:16-21 pubmed
Smith M, Margiotta J, Franco A, Lindstrom J, Berg D. Cholinergic modulation of an acetylcholine receptor-like antigen on the surface of chick ciliary ganglion neurons in cell culture. J Neurosci. 1986;6:946-53 pubmed

product information

Internal ID :

3009

Name :

mAb 35

Depositor Name :

Lindstrom, J.

Depositor Institution :

University of Pennsylvania

Date Deposited :

2/7/89

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

acetylcholine nicotinic receptors, muscle

Antigen Species :

Electrophorus

Host Species :

rat

Isotype :

RIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Bovine,Chicken,Electrophorus,Fish,Human,Mouse,Rat,Zebrafish

Species Tested (legacy) :

zebrafish, fish

Initial Publication Pubmed ID :

6167581

Depositor Notes (Special Instructions) :

acetylcholine binding (alpha) subunits of muscle nicotinic receptors, structural subunits of one subtype of neuronal nicotinic receptors from chicken brain; muscle nicotinic receptors of most species, receptors; immunohistology: from chicken brain; binds to the main immunogenic region on the extracellular surface of alpha subunits of muscle nicotinic receptors; binds to muscle nicotinic receptors from Electrophorus, Torpedo, Rana, chickens, rats, mice, humans, etc., but not to Xenopus. Binds to some neuronal nicotinic receptor subtypes in chickens and finches and cattle, but not to other subtypes in chickens, rats, cattle, and humans; Muscle nicotinic receptors are composed of 4 kinds of subunits with the stoichiometry alpha(2)beta gamma delta., Sites that bind acetylcholine and alpha-bungarotoxin are on the alpha subunits. Neuronal; nicotinic receptors are composed of 2 kinds of subunits, acetylcholine-binding subunits and structural subunits. There are several subtypes of neuronal nicotinic receptors, but none bind alpha-bungarotoxin.; mAb 35 has high affinity for native receptors; mAb 35 has very low affinity for denatured receptors and not good for blotting; mAb 35 can be used for affinity purification; cDNA sequences for the subunits of nicotinic receptors f(more. )

Collections :

Cell signaling,Human,Neurodevelopment,Skeletal muscle

Search Keywords :

Lindstrom, acetylcholine nicotinic receptors, muscle, rat, AChE, mab35

Antibody Registry ID :

AB_528405

Immunogen :

muscle-type nicotinic acetylcholine receptors (Electrophorus electric organ); native protein, adult

Clonality :

Monoclonal

Myeloma Strain :

mouse S194 5.XX0.BU.1

Epitope Mapped :

Recommended Applications :

Function Blocking,Immunohistochemistry,Immunoprecipitation

Immunoblotting (legacy) :

not good

Immunohistochemistry Pubmed IDs :

15212431

Pubmed IDs :

6948300 3209741 2422330 3611197 3694260 2471333 9662653

Additional Information :

RRID:AB_528405

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Biol. Chem. 256, 8635-8645.; PNAS USA 79, 188-192.; In “Hybridoma Technology in the Biosciences and Medicine” (T.A. Springer, ed.), pp. 223-238. Plenum Press, New York.; J. Comp. Neurol. 274, 255-264.; J. Neurosci. 6, 946-953.; J. Cell Biol. 105, 9-18.; J. Neurosci. 7, 4005-4016.; Trends in Neurosci. 12, 16-21.; Cell Tissue Res. 293, 305-311.

more info or order :

Developmental Studies Hybridoma Bank product webpage