F-actin capping protein beta-1 subunit | Developmental Studies Hybridoma Bank mAb 1E5.25.4 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

F-actin capping protein beta-1 subunit

catalog :

mAb 1E5.25.4

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

mAb 1E5.25.4

reactivity :

human, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

product information

Internal ID :

1843

Name :

mAb 1E5.25.4

Depositor Name :

Cooper, J.A.

Depositor Institution :

Washington University Med. School, St. Louis

Depositor Website :

https://cooperlab.wustl.edu/

Date Deposited :

2/13/96

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

F-actin capping protein beta-1 subunit

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Chicken,Human

Species Tested (legacy) :

chicken

Initial Publication Pubmed ID :

1370838

Depositor Notes (Special Instructions) :

For immunofluorescence staining, fixation with a glyoxal-based solution has been successful (PMID: 24319057/ 28899994) while paraformaldehyde has generally not succeeded. Glyoxal solutions can be prepared and used as described (PMID: 29146773) and are commercially available under the name GlyoFixx.

Collections :

Cytoskeleton,Human,Neurodevelopment,Skeletal muscle

Search Keywords :

Cooper, J.A., F-actin capping protein beta-1 subunit, Chicken, MIgG1, kappa light chain, Chicken/Human, CAPZB, CAPB, CAPPB, CAPZ, , Monoclonal, Cytoskeleton/Skeletal muscle/Human/Neurodevelopment, Immunoprecipitation/Western blot/Immunohistochemistry/Function Blocking/Immunofluorescence

Antigen Molecular Weight :

31.3 kDa

Gene :

CAPZB

Alternate Gene Name(s) :

CAPB, CAPPB, CAPZ

Uniprot ID :

P14315

Immunogen :

Recombinant GST-C-terminus beta-1

Immunogen Sequence :

a.a. 251-277

Clonality :

Monoclonal

Myeloma Strain :

P3x63 Ag8.653

Epitope Mapped :

Recommended Applications :

Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

7929588 7822423 18272787 16080205 9331217 24319057

Western Blot Pubmed IDs :

1370838 8858171 7929588 7822423 16987810 18272787 26429793 9331217 24319057

Immunoprecipitation Pubmed IDs :

1370838 16987810

Function Blocking Pubmed IDs :

1370838 8858171 7822423 12690097

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 116(4), 923-931.

more info or order :

Developmental Studies Hybridoma Bank product webpage