product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Integrin, alpha 1/ CD49a /VLA-1
catalog :
mAB 3A3
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
mAB 3A3
reactivity :
rat, chicken
application :
immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 12
Reference
Peng H, Shah W, Holland P, Carbonetto S. Integrins and dystroglycan regulate astrocyte wound healing: the integrin beta1 subunit is necessary for process extension and orienting the microtubular network. Dev Neurobiol. 2008;68:559-74 pubmed publisher
Gotwals P, Chi Rosso G, Lindner V, Yang J, Ling L, Fawell S, et al. The alpha1beta1 integrin is expressed during neointima formation in rat arteries and mediates collagen matrix reorganization. J Clin Invest. 1996;97:2469-77 pubmed
Tawil N, Wilson P, Carbonetto S. Integrins in point contacts mediate cell spreading: factors that regulate integrin accumulation in point contacts vs. focal contacts. J Cell Biol. 1993;120:261-71 pubmed
Tomaselli K, Doherty P, Emmett C, Damsky C, Walsh F, Reichardt L. Expression of beta 1 integrins in sensory neurons of the dorsal root ganglion and their functions in neurite outgrowth on two laminin isoforms. J Neurosci. 1993;13:4880-8 pubmed
Fernandez Valle C, Gwynn L, Wood P, Carbonetto S, Bunge M. Anti-beta 1 integrin antibody inhibits Schwann cell myelination. J Neurobiol. 1994;25:1207-26 pubmed
Loster K, Voigt S, Heidrich C, Hofmann W, Reutter W. Cell-collagen adhesion is inhibited by monoclonal antibody 33.4 against the rat alpha 1-integrin subunit. Exp Cell Res. 1994;212:155-60 pubmed
Keshmirian J, Bray G, Carbonetto S. The extracellular matrix modulates the response of PC12 cells to nerve growth factor: cell aggregation versus neurite outgrowth on 3-dimensional laminin substrata. J Neurocytol. 1989;18:491-504 pubmed
Turner D, Flier L, Carbonetto S. Identification of a cell-surface protein involved in PC12 cell-substratum adhesion and neurite outgrowth on laminin and collagen. J Neurosci. 1989;9:3287-96 pubmed
Ignatius M, Large T, Houde M, Tawil J, Barton A, Esch F, et al. Molecular cloning of the rat integrin alpha 1-subunit: a receptor for laminin and collagen. J Cell Biol. 1990;111:709-20 pubmed
Tawil N, Houde M, Blacher R, Esch F, Reichardt L, Turner D, et al. Alpha 1 beta 1 integrin heterodimer functions as a dual laminin/collagen receptor in neural cells. Biochemistry. 1990;29:6540-4 pubmed
Clyman R, Turner D, Kramer R. An alpha 1/beta 1-like integrin receptor on rat aortic smooth muscle cells mediates adhesion to laminin and collagen types I and IV. Arteriosclerosis. 1990;10:402-9 pubmed
Toyota B, Carbonetto S, David S. A dual laminin/collagen receptor acts in peripheral nerve regeneration. Proc Natl Acad Sci U S A. 1990;87:1319-22 pubmed
product information
Internal ID :
24094
Name :
mAB 3A3
Depositor Name :
Carbonetto, Salvatore / Turner, D.C.
Depositor Institution :
McGill University
Depositor Website :
https://www.mcgill.ca/crn/research/carbonetto
Date Deposited :
9/29/15
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Integrin, alpha 1/ CD49a /VLA-1
Antigen Species :
Rat
Host Species :
mouse
Isotype :
MIgG1
Positive Tested Species Reactivity :
Chick,Rat
Initial Publication Pubmed ID :
2552042
Depositor Notes (Special Instructions) :
mAB 3A3 promotes neurite retraction in PC12 cells and actively inhibits binding of several rat cell types to laminin and collagen. This antibody also labels focal contacts in rat astrocytes and fibroblasts.
Collections :
Cell adhesion,Neurodevelopment
Search Keywords :
Carbonetto, Salvatore, Integrin, alpha 1/ CD49a /VLA-1, Rat, MIgG1, Rat/Chick, Itga1, , , Monoclonal, Cell adhesion/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Immunoprecipitation/Function blocking, turner
Antigen Molecular Weight :
185 kDa /125 kDa
Gene :
Itga1
Uniprot ID :
P18614
Antibody Registry ID :
AB_2722115
Immunogen :
PC12 cells
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
Yes
Epitope Location or Sequence :
Ser-Gly-Pro-Val-Glu-Asp-Met-Phe-Gly-Tyr-Thr-Val.
Epitope Map PubMed ID :
2169872
Recommended Applications :
Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation
Immunohistochemistry Pubmed IDs :
8647938
Immunofluorescence Pubmed IDs :
2169872 8416993 2154740 7513646 7529296
Immunoprecipitation Pubmed IDs :
8416993 2552042 2380249 7693896 2160805
Function Blocking Pubmed IDs :
2553876 2552042 2154740 2169872 8416993 18188865 7529296 8647938 2160805
Depositor Growth Medium :
Iscove's DMEM plus 20% FCS plus supplements
Additional Information :
mAB 3A3 inhibits interaction with the E1 laminin fragment (PMID: 2380249). RRID:AB_2722115
DSHB Growth Medium :
Iscove's
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US