synaptotagmin-1, cytoplasmic domain | Developmental Studies Hybridoma Bank mAB 30 (asv30) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

synaptotagmin-1, cytoplasmic domain

catalog :

mAB 30 (asv30)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

mAB 30 (asv30)

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:50; loading ...; fig s6a	Wang Y, Figueiredo D, Sun X, Dong Z, Chen W, Cui W, et al. Controlling of glutamate release by neuregulin3 via inhibiting the assembly of the SNARE complex. Proc Natl Acad Sci U S A. 2018;115:2508-2513 pubmed publisher
immunohistochemistry; mouse; 1:100; fig 8	Adams K, Rousso D, Umbach J, Novitch B. Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells. Nat Commun. 2015;6:6778 pubmed publisher
	Jiao H, Sun X, Bates R, Xiong L, Zhang L, Liu F, et al. Transmembrane protein 108 is required for glutamatergic transmission in dentate gyrus. Proc Natl Acad Sci U S A. 2017;114:1177-1182 pubmed publisher
	Broguiere N, Isenmann L, Zenobi Wong M. Novel enzymatically cross-linked hyaluronan hydrogels support the formation of 3D neuronal networks. Biomaterials. 2016;99:47-55 pubmed publisher
	Mannari T, Morita S, Furube E, Tominaga M, Miyata S. Astrocytic TRPV1 ion channels detect blood-borne signals in the sensory circumventricular organs of adult mouse brains. Glia. 2013;61:957-71 pubmed publisher
	Podrygajlo G, Tegenge M, Gierse A, Paquet Durand F, Tan S, Bicker G, et al. Cellular phenotypes of human model neurons (NT2) after differentiation in aggregate culture. Cell Tissue Res. 2009;336:439-52 pubmed publisher
	Matthew W, Tsavaler L, Reichardt L. Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue. J Cell Biol. 1981;91:257-69 pubmed
	Greif K, Reichardt L. Appearance and distribution of neuronal cell surface and synaptic vesicle antigens in the developing rat superior cervical ganglion. J Neurosci. 1982;2:843-52 pubmed

product information

Internal ID :

1487

Name :

mAB 30 (asv30)

Depositor Name :

Reichardt, L.

Depositor Institution :

Department of Physiology, University of California

Date Deposited :

4/20/99

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

synaptotagmin-1, cytoplasmic domain

Antigen Species :

rat

Host Species :

mouse

Isotype :

MIgG2a, kappa light chain

Isotype for catalog (legacy) :

IgG2a, kappa light chain

Positive Tested Species Reactivity :

Chicken,Fish,Human,Mouse,Rat,Xenopus

Species Tested (legacy) :

fish, Xenopus, chick, mouse, rat and human

Initial Publication Pubmed ID :

7298720

Depositor Notes (Special Instructions) :

mAB 30 (asv30) as a lower affinity than mAb 48 (asv 48). The two antibodies recognize the same antigen, Syt1. mAB 30 (asv30) is detergent sensitive: TritonX-100 sensitive epitope (reversible), epitope present in presence of CHAPS.

Collections :

Cell markers,Human,Neurodevelopment

Search Keywords :

Reichardt, L., synaptotagmin-1, cytoplasmic domain, rat, MIgG2a, kappa light chain, fish/Xenopus/chicken/mouse/rat/human , Syt1, P65, AB_2295002, monoclonal, Human/Neurodevelopment/Cell markers, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 47 kDa; Apparent: 65 kDa

Gene :

Syt1

Alternate Gene Name(s) :

P65

Uniprot ID :

P21707

Antibody Registry ID :

AB_2295002

Synonyms (Alternate Clone Names) :

SV 30

Immunogen :

Synaptic junctional complex proteins prepared from rat brain synaptic membranes

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

Cytoplasmic (aa 80-421)

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes, detergent sensitive

Immunohistochemistry Pubmed IDs :

7298720 6212651

Immunofluorescence Pubmed IDs :

25868900 27209262 23468425 19377856

Western Blot Pubmed IDs :

7298720 28096412

Immunoprecipitation Pubmed IDs :

7298720

Additional Information :

*Not available to for-profit organizations. For further information please contact the Office of Innovation, Technology, and Alliances at the University of California. Refer to case number 95-082. Email : ita@ucsf.edu Telephone: 415 502-1603. Researchers at non-profit institutions must sign an additional compliance form prior to purchasing this antibody. The compliance form is available by emailing dshb@uiowa.edu or by calling 319 335-3826.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 81, 257-269; J. Neurosci. 2, 843-852.

more info or order :

Developmental Studies Hybridoma Bank product webpage