Kelch | Developmental Studies Hybridoma Bank kel 1B product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Kelch

catalog :

kel 1B

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

kel 1B

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 14

Reference
Hudson A, Szabo N, Loughran G, Wills N, Atkins J, Cooley L. Tissue-specific dynamic codon redefinition in Drosophila. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Kline A, Curry T, Lewellyn L. The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber. Dev Biol. 2018;440:99-112 pubmed publisher
Huelsmann S, Rintanen N, Sethi R, Brown N, Ylanne J. Evidence for the mechanosensor function of filamin in tissue development. Sci Rep. 2016;6:32798 pubmed publisher
Hudson A, Mannix K, Cooley L. Actin Cytoskeletal Organization in Drosophila Germline Ring Canals Depends on Kelch Function in a Cullin-RING E3 Ligase. Genetics. 2015;201:1117-31 pubmed publisher
Hsu S, Plata M, Ernest B, Asgarifar S, Labrador M. The insulator protein Suppressor of Hairy wing is required for proper ring canal development during oogenesis in Drosophila. Dev Biol. 2015;403:57-68 pubmed publisher
Ogienko A, Karagodin D, Lashina V, Baiborodin S, Omelina E, Baricheva E. Capping protein beta is required for actin cytoskeleton organisation and cell migration during Drosophila oogenesis. Cell Biol Int. 2013;37:149-59 pubmed publisher
Morawe T, Honemann Capito M, von Stein W, Wodarz A. Loss of the extraproteasomal ubiquitin receptor Rings lost impairs ring canal growth in Drosophila oogenesis. J Cell Biol. 2011;193:71-80 pubmed publisher
Hudson A, Cooley L. Drosophila Kelch functions with Cullin-3 to organize the ring canal actin cytoskeleton. J Cell Biol. 2010;188:29-37 pubmed publisher
Kloc M, Bilinski S, Dougherty M, Brey E, Etkin L. Formation, architecture and polarity of female germline cyst in Xenopus. Dev Biol. 2004;266:43-61 pubmed
Kelso R, Hudson A, Cooley L. Drosophila Kelch regulates actin organization via Src64-dependent tyrosine phosphorylation. J Cell Biol. 2002;156:703-13 pubmed
Robinson D, Cooley L. Drosophila kelch is an oligomeric ring canal actin organizer. J Cell Biol. 1997;138:799-810 pubmed
Robinson D, Cooley L. Examination of the function of two kelch proteins generated by stop codon suppression. Development. 1997;124:1405-17 pubmed
Xue F, Cooley L. kelch encodes a component of intercellular bridges in Drosophila egg chambers. Cell. 1993;72:681-93 pubmed
Robinson D, Cant K, Cooley L. Morphogenesis of Drosophila ovarian ring canals. Development. 1994;120:2015-25 pubmed

product information

Internal ID :

1831

Name :

kel 1B

Depositor Name :

Cooley, L.

Depositor Institution :

Yale Medical School

Date Deposited :

7/12/01

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Kelch

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila,Drosophila melanogaster,Xenopus laevis

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

8453663

Depositor Notes (Special Instructions) :

Use 1:10 in Blotto-Tween (PBS, 5% dry milk, 0.2% Tween) for Westerns or 1:1 in PBT (PBS, 0.3% Triton-X-100, and 0.5% BSA) for ovary staining. (Xue and Cooley, 1993). This antibody doesn't appear to work for immunoprecipitation. No immunoreactivity in mutant fly.

Collections :

Cell signaling,Cytoskeleton,Drosophila antigens,Xenopus

Search Keywords :

Cooley, L., Kelch, Drosophila, MIgG1, kappa light chain, Drosophila melanogaster/Drosophila/Xenopus laevis, kel, CG7210, Kelch, AB_528318, monoclonal, Cell signaling/Cytoskeletal elements/Drosophila antigens/Xenopus, Immunohistochemistry/Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: 77 kDa (short), 160 kDa (full lenght); Apparent: ~80 kDa (short), ~180 kDa (full length)

Gene :

kel

Alternate Gene Name(s) :

CG7210, Kelch

Uniprot ID :

Q04652

Antibody Registry ID :

AB_528318

Synonyms (Alternate Clone Names) :

9C, kelch 1B

Immunogen :

Recombinant Kelch (first 330 aa of N-terminus)/GST fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Epitope Mapped :

Yes

Epitope Location or Sequence :

BTB domain (aa 111-230)

Epitope Map PubMed ID :

9265647

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

14729477

Immunofluorescence Pubmed IDs :

8453663 9265647 7925006 9118811 11854310 20065088 26384358 14729477 25882370 23339103 27597179 29753016

Western Blot Pubmed IDs :

8453663 9265647 9118811 11854310 20065088 26384358

Additional Information :

Kelch is a component of ring canals that regulates the flow of cytoplasm between cells. kel 1B recognizes both the short and full lenght Kelch protein.

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 72, 681-693.; Cell Biol. Int. 37, 149-159.

more info or order :

Developmental Studies Hybridoma Bank product webpage