hu-li tai shao, hts-RC | Developmental Studies Hybridoma Bank hts RC product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

hu-li tai shao, hts-RC

catalog :

hts RC

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

hts RC

reactivity :

fruit fly

application :

western blot, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 16

Reference
Kline A, Curry T, Lewellyn L. The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber. Dev Biol. 2018;440:99-112 pubmed publisher
Lie Jensen A, Haglund K. Antibody Staining in Drosophila Germaria. Methods Mol Biol. 2016;1457:19-33 pubmed publisher
O Neill R, Clark D. Partial Functional Diversification of Drosophila melanogaster Septin Genes Sep2 and Sep5. G3 (Bethesda). 2016;6:1947-57 pubmed publisher
Hsu S, Plata M, Ernest B, Asgarifar S, Labrador M. The insulator protein Suppressor of Hairy wing is required for proper ring canal development during oogenesis in Drosophila. Dev Biol. 2015;403:57-68 pubmed publisher
Wang S, Tsai A, Wang M, Yoo S, Kim H, Yoo B, et al. Phospho-regulated Drosophila adducin is a determinant of synaptic plasticity in a complex with Dlg and PIP2 at the larval neuromuscular junction. Biol Open. 2014;3:1196-206 pubmed publisher
Huelsmann S, Ylanne J, Brown N. Filopodia-like actin cables position nuclei in association with perinuclear actin in Drosophila nurse cells. Dev Cell. 2013;26:604-15 pubmed publisher
Azzam G, Smibert P, Lai E, Liu J. Drosophila Argonaute 1 and its miRNA biogenesis partners are required for oocyte formation and germline cell division. Dev Biol. 2012;365:384-94 pubmed publisher
Strong T, Kaur G, Thomas J. Mutations in the catalytic loop HRD motif alter the activity and function of Drosophila Src64. PLoS ONE. 2011;6:e28100 pubmed publisher
Morawe T, Honemann Capito M, von Stein W, Wodarz A. Loss of the extraproteasomal ubiquitin receptor Rings lost impairs ring canal growth in Drosophila oogenesis. J Cell Biol. 2011;193:71-80 pubmed publisher
Gorjanacz M, Török I, Pomozi I, Garab G, Szlanka T, Kiss I, et al. Domains of Importin-alpha2 required for ring canal assembly during Drosophila oogenesis. J Struct Biol. 2006;154:27-41 pubmed
Wahlström G, Lahti V, Pispa J, Roos C, Heino T. Drosophila non-muscle alpha-actinin is localized in nurse cell actin bundles and ring canals, but is not required for fertility. Mech Dev. 2004;121:1377-91 pubmed
Dodson G, Guarnieri D, Simon M. Src64 is required for ovarian ring canal morphogenesis during Drosophila oogenesis. Development. 1998;125:2883-92 pubmed
Robinson D, Cooley L. Drosophila kelch is an oligomeric ring canal actin organizer. J Cell Biol. 1997;138:799-810 pubmed
Robinson D, Cooley L. Examination of the function of two kelch proteins generated by stop codon suppression. Development. 1997;124:1405-17 pubmed
Robinson D, Smith Leiker T, Sokol N, Hudson A, Cooley L. Formation of the Drosophila ovarian ring canal inner rim depends on cheerio. Genetics. 1997;145:1063-72 pubmed
Robinson D, Cant K, Cooley L. Morphogenesis of Drosophila ovarian ring canals. Development. 1994;120:2015-25 pubmed

product information

Internal ID :

1825

Name :

hts RC

Depositor Name :

Cooley, L.

Depositor Institution :

Yale Medical School

Date Deposited :

7/12/01

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

hu-li tai shao, hts-RC

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

7925006

Depositor Notes (Special Instructions) :

Use 1:10 in Blotto-Tween (PBS, 5% dry milk, 0.2% Tween) for Westerns or 1:1 in PBT (PBS, 0.3% Triton-X-100, and 0.5% BSA) for ovary staining. (Robinson et al., 1994). Identifies an epitope on the ring canal. Doesn’t work for Immunoprecipitation.

Collections :

Cell signaling,Cytoskeleton,Drosophila antigens

Search Keywords :

Cooley, L., hu-li tai shao, hts-RC, Drosophila, MIgG1, kappa light chain, Drosophila, hts, CG43443, Add, adducin, hu li tai shao, add-like, add-hts, Ovhts, AB_528289, monoclonal, Cell signaling/Cytoskeletal elements/Drosophila antigens, Immunofluorescence/Western Blot

Antigen Molecular Weight :

Apparent: 55 and 68 kDa have been reported

Gene :

hts

Alternate Gene Name(s) :

CG43443, Add, adducin, hu li tai shao, add-like, add-hts, Ovhts

Uniprot ID :

Q02645

Antibody Registry ID :

AB_528289

Synonyms (Alternate Clone Names) :

anti-hts 655 4B

Immunogen :

Recombinant Drosophila hu-li tai shao protein (351 aa C-terminus of hts, isoform N4)/GST fusion.

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Epitope Mapped :

Yes

Epitope Location or Sequence :

351 aa C-terminal of isoform A (N4; Uniprot ID: Q02645-1) spanning aa 806-1156.

Recommended Applications :

Immunofluorescence,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

7925006 9655810 9118811 9093858 27172205 27557571 25882370 9265647 15454267 21444692 16458020 22445511 22132220 29753016

Western Blot Pubmed IDs :

7925006 9118811 9093858 21444692

Additional Information :

hts RC will not recognize any isoform missing the C-terminal portion of isoform N4. Rcognizes Ovhts-RC.

DSHB Growth Medium :

Iscove's

References (legacy) :

Development 120, 2015-2025.; Dev. Biol. 365, 384-394.

more info or order :

Developmental Studies Hybridoma Bank product webpage