product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Profilin/chickadee
catalog :
chi 1J
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
chi 1J
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 18
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; fruit fly; fig s3a
  • western blot; fruit fly; 1:10; fig s3m
Groen C, Jayo A, Parsons M, Tootle T. Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture. Mol Biol Cell. 2015;26:1901-17 pubmed publisher
  • immunohistochemistry; fruit fly; 1:1
Fairchild M, Smendziuk C, Tanentzapf G. A somatic permeability barrier around the germline is essential for Drosophila spermatogenesis. Development. 2015;142:268-81 pubmed publisher
Valoskova K, Biebl J, Roblek M, Emtenani S, Gyoergy A, Misova M, et al. A conserved major facilitator superfamily member orchestrates a subset of O-glycosylation to aid macrophage tissue invasion. elife. 2019;8: pubmed publisher
Jang B, Ryoo H, Son J, Choi K, Shin D, Kang S, et al. Role of Drosophila EDEMs in the degradation of the alpha-1-antitrypsin Z variant. Int J Mol Med. 2015;35:870-6 pubmed publisher
Shields A, Spence A, Yamashita Y, Davies E, Fuller M. The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells. Development. 2014;141:73-82 pubmed publisher
Urwyler O, Cortinas Elizondo F, Suter B. Drosophila sosie functions with ?(H)-Spectrin and actin organizers in cell migration, epithelial morphogenesis and cortical stability. Biol Open. 2012;1:994-1005 pubmed publisher
Brock A, Wang Y, Berger S, Renkawitz Pohl R, Han V, Wu Y, et al. Transcriptional regulation of Profilin during wound closure in Drosophila larvae. J Cell Sci. 2012;125:5667-76 pubmed publisher
Chu D, Pan H, Wan P, Wu J, Luo J, Zhu H, et al. AIP1 acts with cofilin to control actin dynamics during epithelial morphogenesis. Development. 2012;139:3561-71 pubmed publisher
Coffee R, Williamson A, Adkins C, Gray M, Page T, Broadie K. In vivo neuronal function of the fragile X mental retardation protein is regulated by phosphorylation. Hum Mol Genet. 2012;21:900-15 pubmed publisher
Domingues C, Ryoo H. Drosophila BRUCE inhibits apoptosis through non-lysine ubiquitination of the IAP-antagonist REAPER. Cell Death Differ. 2012;19:470-7 pubmed publisher
Gonçalves Pimentel C, Gombos R, Mihály J, Sánchez Soriano N, Prokop A. Dissecting regulatory networks of filopodia formation in a Drosophila growth cone model. PLoS ONE. 2011;6:e18340 pubmed publisher
Burnett B, Andrews J, Ranganathan S, Fischbeck K, Di Prospero N. Expression of expanded polyglutamine targets profilin for degradation and alters actin dynamics. Neurobiol Dis. 2008;30:365-74 pubmed publisher
Tessier C, Broadie K. Drosophila fragile X mental retardation protein developmentally regulates activity-dependent axon pruning. Development. 2008;135:1547-57 pubmed publisher
Rosin D, Schejter E, Volk T, Shilo B. Apical accumulation of the Drosophila PDGF/VEGF receptor ligands provides a mechanism for triggering localized actin polymerization. Development. 2004;131:1939-48 pubmed
Rogers S, Wiedemann U, Stuurman N, Vale R. Molecular requirements for actin-based lamella formation in Drosophila S2 cells. J Cell Biol. 2003;162:1079-88 pubmed
Verheyen E, Cooley L. Profilin mutations disrupt multiple actin-dependent processes during Drosophila development. Development. 1994;120:717-28 pubmed
Karasek M. [Morphologic and histochemical studies of the pineal gland in white rats during regeneration of the adrenals]. Endokrynol Pol. 1968;19:549-60 pubmed
Webster C, Silberstein L, Hays A, Blau H. Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy. Cell. 1988;52:503-13 pubmed
product information
Internal ID :
1819
Name :
chi 1J
Depositor Name :
Cooley, L.
Depositor Institution :
Yale University
Depositor Website :
http://cooley.medicine.yale.edu/
Date Deposited :
7/12/01
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Profilin/chickadee
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
7600952
Depositor Notes (Special Instructions) :
Drosophila profilin is also known as protein chickadee.
Collections :
Cytoskeleton,Drosophila antigens
Search Keywords :
Cooley, L., Profilin/chickadee, Drosophila, MIgG1, kappa light chain, Drosophila, chic, chi, AB_528439, Monoclonal, Drosophila antigens/Cytoskeletal elements, Immunohistochemistry/Immunofluorescence/Western blot
Antigen Molecular Weight :
13.7 kDa
Gene :
chic
Alternate Gene Name(s) :
chi
Uniprot ID :
P25843
Antibody Registry ID :
AB_528439
Immunogen :
Recombinant profilin fusion protein
Immunogen Sequence :
Full length sequence
Clonality :
Monoclonal
Myeloma Strain :
SP2/2
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
3342447
Immunofluorescence Pubmed IDs :
3342447 25503408 24346697 22976306 23213377 22899846 21464901 18417352 15056618 12975351
Western Blot Pubmed IDs :
7600952 5716426 21886178 22080836 18417352 18321984
Additional Information :
RRID:AB_528439 FBgn0000308
DSHB Growth Medium :
Iscove's
References (legacy) :
Development 120, 717-728.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US