Notch1 intracellular domain (human) | Developmental Studies Hybridoma Bank bTAN 20 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Notch1 intracellular domain (human)

catalog :

bTAN 20

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

bTAN 20

reactivity :

human, mouse, dogs

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
western blot; human	Baldoni S, Del Papa B, Dorillo E, Aureli P, De Falco F, Rompietti C, et al. Bepridil exhibits anti-leukemic activity associated with NOTCH1 pathway inhibition in chronic lymphocytic leukemia. Int J Cancer. 2018;143:958-970 pubmed publisher
western blot; mouse; loading ...; fig 2b	Fujiwara T, Zhou J, Ye S, Zhao H. RNA-binding protein Musashi2 induced by RANKL is critical for osteoclast survival. Cell Death Dis. 2016;7:e2300 pubmed publisher
	Agabiti S, Li J, Dong W, Poe M, Wiemer A. Regulation of the Notch-ATM-abl axis by geranylgeranyl diphosphate synthase inhibition. Cell Death Dis. 2019;10:733 pubmed publisher
	Saha M, Mitsuhashi S, Jones M, Manko K, Reddy H, Bruels C, et al. Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions. Hum Mol Genet. 2017;26:2984-3000 pubmed publisher
	Bauer L, Takacs A, Slotta Huspenina J, Langer R, Becker K, Novotny A, et al. Clinical Significance of NOTCH1 and NOTCH2 Expression in Gastric Carcinomas: An Immunohistochemical Study. Front Oncol. 2015;5:94 pubmed publisher
	Zhou J, Fujiwara T, Ye S, Li X, Zhao H. Ubiquitin E3 Ligase LNX2 is Critical for Osteoclastogenesis In Vitro by Regulating M-CSF/RANKL Signaling and Notch2. Calcif Tissue Int. 2015;96:465-75 pubmed publisher
	Kannan S, Sutphin R, Hall M, Golfman L, Fang W, Nolo R, et al. Notch activation inhibits AML growth and survival: a potential therapeutic approach. J Exp Med. 2013;210:321-37 pubmed publisher
	Meliou E, Kerezoudis N, Tosios K, Kiaris H. Notch 1 Receptor, Delta 1 Ligand and HES 1 Transcription Factor are Expressed in the Lining Epithelium of Periapical Cysts (Preliminary Study). Open Dent J. 2010;4:153-8 pubmed publisher
	Watanabe K, Nagaoka T, Lee J, Bianco C, Gonzales M, Castro N, et al. Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1. J Cell Biol. 2009;187:343-53 pubmed publisher
	Fox V, Gokhale P, Walsh J, Matin M, Jones M, Andrews P. Cell-cell signaling through NOTCH regulates human embryonic stem cell proliferation. Stem Cells. 2008;26:715-23 pubmed
	Shimizu T, Nakano K, Tsujigiwa H, Nagatsuka H, Watanabe T, Okafuji N, et al. Notch signaling in mandibular condylar cartilage development. Eur J Med Res. 2007;12:515-9 pubmed
	Shimizu T, Tsujigiwa H, Nagatsuka H, Okafuji N, Kurihara S, Nagai N, et al. Expression of Notch1 and Math1 in mandibular condyle cartilage in neonatal mice. Angle Orthod. 2005;75:993-5 pubmed
	Kawakami T, Shimizu T, Kimura A, Hasegawa H, Siar C, Ng K, et al. Immunohistochemical examination of cytological differentiation in osteosarcomas. Eur J Med Res. 2005;10:475-9 pubmed
	Chappell W, Green T, Spengeman J, McCubrey J, Akula S, Bertrand F. Increased protein expression of the PTEN tumor suppressor in the presence of constitutively active Notch-1. Cell Cycle. 2005;4:1389-95 pubmed
	Kawakami T, Siar C, Ng K, Shimizu T, Okafuji N, Kurihara S, et al. Expression of Notch in a case of osteosarcoma of the maxilla. Eur J Med Res. 2004;9:533-5 pubmed
	Sade H, Krishna S, Sarin A. The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells. J Biol Chem. 2004;279:2937-44 pubmed
	Jundt F, Anagnostopoulos I, Forster R, Mathas S, Stein H, Dorken B. Activated Notch1 signaling promotes tumor cell proliferation and survival in Hodgkin and anaplastic large cell lymphoma. Blood. 2002;99:3398-403 pubmed
	Blaumueller C, Qi H, Zagouras P, Artavanis Tsakonas S. Intracellular cleavage of Notch leads to a heterodimeric receptor on the plasma membrane. Cell. 1997;90:281-91 pubmed
	Zagouras P, Stifani S, Blaumueller C, Carcangiu M, Artavanis Tsakonas S. Alterations in Notch signaling in neoplastic lesions of the human cervix. Proc Natl Acad Sci U S A. 1995;92:6414-8 pubmed

product information

Internal ID :

409

Name :

bTAN 20

Depositor Name :

Artavanis-Tsakonas, S.

Depositor Institution :

Harvard Medical School

Date Deposited :

5/2/00

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Notch1 intracellular domain (human)

Antigen Species :

Human

Host Species :

rat

Isotype :

RIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Canine,Human,Mouse

Species Tested (legacy) :

human, mouse, dog

Initial Publication Pubmed ID :

7604005

Depositor Notes (Special Instructions) :

bTAN 20 doesn't work well for immunoprecipitation

Collections :

Cardiac development,Cell adhesion,Cell signaling,Human,Neurodevelopment,Skeletal muscle,Transcription factors

Search Keywords :

Artavanis-Tsakonas, S., Notch1 intracellular domain (human), Human, RIgG1, kappa light chain, human/mouse/canine, NOTCH1, hN1, AOS5, TAN1, AOVD1, AB_2153497, monoclonal, Cardiac development/Cell adhesion/Cell signaling/Human/Transcription factors/Neurodevelopment/Skeletal muscle, Immunohistochemistry/Immunofluorescence/Western Blot

Antigen Molecular Weight :

Apparent: 300 kDa processed to give a major intracellular product of 110-120 kDa

Gene :

NOTCH1

Alternate Gene Name(s) :

hN1, AOS5, TAN1, AOVD1

Uniprot ID :

P46531

Antibody Registry ID :

AB_2153497

Synonyms (Alternate Clone Names) :

15A

Immunogen :

Recombinant fusion protein GST-Notch1 intrcellular domain

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8.653 (ATCC CRL-1580)

Epitope Mapped :

Yes

Epitope Location or Sequence :

intracellular domain

Epitope Map PubMed ID :

7604005

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Excellent

Immunohistochemistry Pubmed IDs :

7604005 15649865 16354601 16448243 18024259 21116324 11964309

Immunofluorescence Pubmed IDs :

7604005 18055449 28498977 25712254

Western Blot Pubmed IDs :

7604005 9244302 11964309 14583609 16096376 18055449 28498977

DSHB Growth Medium :

RPMI

References (legacy) :

Proc. Natl. Acad. Sci. 92, 6414-6418.; Cell 90, 281-291.; Eur. J. Med. Res. 9, 533-535.; Eur. J. Med. Res. 10, 475-479.; Angle Orthod. 75(6), 993-995.; Eur. J. Med. Res. 12, 515-519.; Open Dent. J. 4, 153-158.

more info or order :

Developmental Studies Hybridoma Bank product webpage