ATPase, (Na(+) K(+)) beta-1 subunit (avian) | Developmental Studies Hybridoma Bank b4B4 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

ATPase, (Na(+) K(+)) beta-1 subunit (avian)

catalog :

b4B4

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

b4B4

reactivity :

chicken

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 2

Reference
Fadool J, Linser P. Evidence for the formation of multimeric forms of the 5A11/HT7 antigen. Biochem Biophys Res Commun. 1996;229:280-6 pubmed
Wolitzky B, Fambrough D. Regulation of the (Na+ + K+)-ATPase in cultured chick skeletal muscle. Modulation of expression by the demand for ion transport. J Biol Chem. 1986;261:9990-9 pubmed

product information

Internal ID :

2701

Name :

b4B4

Depositor Name :

Fambrough, D.M.

Depositor Institution :

Johns Hopkins School of Medicine

Depositor Website :

http://www.searlescholars.net/person/470

Date Deposited :

10/10/90

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

ATPase, (Na(+) K(+)) beta-1 subunit (avian)

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Avian

Species Tested (legacy) :

avian; cross-reactivity with mammalian unlikely

Initial Publication Pubmed ID :

3015915

Depositor Notes (Special Instructions) :

This antibody appears to recognize a linear epitope of the beta1-subunit of Na, K-ATPase exposed on the cell surface. Immunohistochemistry: works well on cryosections. Cros-reactivity with mammalian is unlikely.

Collections :

Cardiac development,Enzymes,Neurodevelopment,Skeletal muscle

Search Keywords :

Fambrough, D. M., ATPase, (Na(+) K(+)) beta-1 subunit (avian), Chicken, MIgG2a, Avian, ATP1B1, , AB_528095, monoclonal, Cardiac development/Enzymes/Neurodevelopment/Skeletal muscle, Immunohistochemistry/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 35 kDa; Apparent: broad band at 45-60 kDa

Gene :

ATP1B1

Uniprot ID :

P08251

Antibody Registry ID :

AB_528095

Synonyms (Alternate Clone Names) :

beta4B4; mAb 4B4

Immunogen :

purified adult chicken kidney (Na+, K+)-ATPase beta-subunit.

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0 Ag8

Epitope Mapped :

Recommended Applications :

Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Western Blot Pubmed IDs :

8954119

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Biol. Chem. 261, 9990-9999.; Exp. Eye Res. 53, 549-553.

more info or order :

Developmental Studies Hybridoma Bank product webpage