ATPase, (Na(+) K(+)) beta-1 subunit (avian) | Developmental Studies Hybridoma Bank b29 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

ATPase, (Na(+) K(+)) beta-1 subunit (avian)

catalog :

b29

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

b29

reactivity :

chicken

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 4

Reference
Zhou X, Fambrough D. Expression of the avian Na,K-ATPase subunits in Dictyostelium discoideum. J Membr Biol. 1999;167:19-24 pubmed
Emerick M, Fambrough D. Intramolecular fusion of Na pump subunits assures exclusive assembly of the fused alpha and beta subunit domains into a functional enzyme in cells also expressing endogenous Na pump subunits. J Biol Chem. 1993;268:23455-9 pubmed
Hamrick M, Renaud K, Fambrough D. Assembly of the extracellular domain of the Na,K-ATPase beta subunit with the alpha subunit. Analysis of beta subunit chimeras and carboxyl-terminal deletions. J Biol Chem. 1993;268:24367-73 pubmed
Renaud K, Inman E, Fambrough D. Cytoplasmic and transmembrane domain deletions of Na,K-ATPase beta-subunit. Effects on subunit assembly and intracellular transport. J Biol Chem. 1991;266:20491-7 pubmed

product information

Internal ID :

2689

Name :

b29

Depositor Name :

Fambrough, D.M.

Depositor Institution :

Johns Hopkins School of Medicine

Depositor Website :

http://www.searlescholars.net/person/470

Date Deposited :

12/1/98

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

ATPase, (Na(+) K(+)) beta-1 subunit (avian)

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Avian

Species Tested (legacy) :

avian

Initial Publication Pubmed ID :

1657927 7901216

Depositor Notes (Special Instructions) :

The epitope appears to be linear. Does not bind native beta subunits on cells, but does bind to some C-terminally truncated forms. This antibody preferentially recognizes partially denatured beta subunits. This antibody is excellent for Immunoblotting but does not work for Immunoprecipitation or Immunohistochemistry. Cros-reactivity with mammalian is unlikely. Weaker than mAb 24 for immunoprecipitation of native beta subunit, but better than mAb 24 for truncated subunit [PMID 7901216].

Collections :

Cardiac development,Enzymes,Neurodevelopment,Skeletal muscle

Search Keywords :

Fambrough, D. M., ATPase, (Na(+) K(+)) beta-1 subunit (avian), Chicken, MIgG1, kappa light chain, Avian, ATP1B1, , AB_528096, monoclonal, Cardiac development/Enzymes/Neurodevelopment/Skeletal muscle, Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 35 kDa; Apparent: broad band at 45-60 kDa

Gene :

ATP1B1

Uniprot ID :

P08251

Antibody Registry ID :

AB_528096

Synonyms (Alternate Clone Names) :

beta29; mAb 29

Immunogen :

purified adult chicken kidney (Na+, K+)-ATPase beta-subunit.

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0 Ag8

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Excellent

Immunofluorescence Pubmed IDs :

7901216 8226871

Western Blot Pubmed IDs :

1657927 9878071

Immunoprecipitation Pubmed IDs :

7901216

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Biol. Chem. 268, 24367-24373.

more info or order :

Developmental Studies Hybridoma Bank product webpage