product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
vasa
catalog :
anti-vasa
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
anti-vasa
reactivity :
Drosophila melanogaster
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 70
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; Drosophila melanogaster; 1:25; loading ...; fig 3h
Valoskova K, Biebl J, Roblek M, Emtenani S, Gyoergy A, Misova M, et al. A conserved major facilitator superfamily member orchestrates a subset of O-glycosylation to aid macrophage tissue invasion. elife. 2019;8: pubmed publisher
Bhargava V, Goldstein C, Russell L, Xu L, Ahmed M, Li W, et al. GCNA Preserves Genome Integrity and Fertility Across Species. Dev Cell. 2020;52:38-52.e10 pubmed publisher
Zhao S, Wu C, Gao Z, Li X, Guo Z, Wang Z. Notch signaling governs the expression of glypican Dally to define the stem cell niche. Biol Open. 2020;9: pubmed publisher
Tiwari M, Zeitler D, Meister G, Wodarz A. Molecular profiling of stem cell-like female germ line cells in Drosophila delineates networks important for stemness and differentiation. Biol Open. 2019;8: pubmed publisher
Kneuss E, Munafò M, Eastwood E, Deumer U, Preall J, Hannon G, et al. Specialization of the Drosophila nuclear export family protein Nxf3 for piRNA precursor export. Genes Dev. 2019;33:1208-1220 pubmed publisher
Kotov A, Adashev V, Godneeva B, Ninova M, Shatskikh A, Bazylev S, et al. piRNA silencing contributes to interspecies hybrid sterility and reproductive isolation in Drosophila melanogaster. Nucleic Acids Res. 2019;47:4255-4271 pubmed publisher
Sreejith P, Jang W, To V, Hun Jo Y, Biteau B, Kim C. Lin28 is a critical factor in the function and aging of Drosophila testis stem cell niche. Aging (Albany NY). 2019;11:855-873 pubmed publisher
Dubey P, Kapoor T, Gupta S, Shirolikar S, Ray K. Atypical septate junctions maintain the somatic enclosure around maturing spermatids and prevent premature sperm release in Drosophila testis. Biol Open. 2019;8: pubmed publisher
Jankovics F, Bence M, Sinka R, Faragó A, Bodai L, Pettkó Szandtner A, et al. Drosophila small ovary gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation. Development. 2018;145: pubmed publisher
McCarthy A, Deiulio A, Martin E, Upadhyay M, Rangan P. Tip60 complex promotes expression of a differentiation factor to regulate germline differentiation in female Drosophila. Mol Biol Cell. 2018;29:2933-2945 pubmed publisher
Volin M, Zohar Fux M, Gonen O, Porat Kuperstein L, Toledano H. microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis. J Cell Biol. 2018;217:3829-3838 pubmed publisher
Huang Y, Moreno H, Row S, Jia D, Deng W. Germline silencing of UASt depends on the piRNA pathway. J Genet Genomics. 2018;: pubmed publisher
Su T, Nakato E, Choi P, Nakato H. Drosophila Glypicans Regulate Follicle Stem Cell Maintenance and Niche Competition. Genetics. 2018;209:537-549 pubmed publisher
Coux R, Teixeira F, Lehmann R. L(3)mbt and the LINT complex safeguard cellular identity in the Drosophila ovary. Development. 2018;145: pubmed publisher
Singh A, Dutta D, Paul M, Verma D, Mutsuddi M, Mukherjee A. Pleiotropic Functions of the Chromodomain-Containing Protein Hat-trick During Oogenesis in Drosophila melanogaster. G3 (Bethesda). 2018;8:1067-1077 pubmed publisher
Yatsenko A, Shcherbata H. Stereotypical architecture of the stem cell niche is spatiotemporally established by miR-125-dependent coordination of Notch and steroid signaling. Development. 2018;145: pubmed publisher
Wang X, Page McCaw A. Wnt6 maintains anterior escort cells as an integral component of the germline stem cell niche. Development. 2018;145: pubmed publisher
Chen D, Zhou L, Sun F, Sun M, Tao X. Cyclin B3 Deficiency Impairs Germline Stem Cell Maintenance and Its Overexpression Delays Cystoblast Differentiation in Drosophila Ovary. Int J Mol Sci. 2018;19: pubmed publisher
Teixeira F, Okuniewska M, Malone C, Coux R, Rio D, Lehmann R. piRNA-mediated regulation of transposon alternative splicing in the soma and germ line. Nature. 2017;: pubmed publisher
Lu K, Yamashita Y. Germ cell connectivity enhances cell death in response to DNA damage in the Drosophila testis. elife. 2017;6: pubmed publisher
LeBlanc M, Lehmann R. Domain-specific control of germ cell polarity and migration by multifunction Tre1 GPCR. J Cell Biol. 2017;216:2945-2958 pubmed publisher
Kan L, Grozhik A, Vedanayagam J, Patil D, Pang N, Lim K, et al. The m6A pathway facilitates sex determination in Drosophila. Nat Commun. 2017;8:15737 pubmed publisher
Tan S, Lee Q, Wong B, Cai Y, Baeg G. Redox Homeostasis Plays Important Roles in the Maintenance of the Drosophila Testis Germline Stem Cells. Stem Cell Reports. 2017;9:342-354 pubmed publisher
Zhang C, Montooth K, Calvi B. Incompatibility between mitochondrial and nuclear genomes during oogenesis results in ovarian failure and embryonic lethality. Development. 2017;144:2490-2503 pubmed publisher
Cook M, Cazin C, Amoyel M, Yamamoto S, Bach E, Nystul T. Neutral Competition for Drosophila Follicle and Cyst Stem Cell Niches Requires Vesicle Trafficking Genes. Genetics. 2017;206:1417-1428 pubmed publisher
Lai C, Lin K, Kao S, Chen Y, Huang F, Hsu H. Hedgehog signaling establishes precursors for germline stem cell niches by regulating cell adhesion. J Cell Biol. 2017;216:1439-1453 pubmed publisher
Tang Y, Geng Q, Chen D, Zhao S, Liu X, Wang Z. Germline Proliferation Is Regulated by Somatic Endocytic Genes via JNK and BMP Signaling in Drosophila. Genetics. 2017;206:189-197 pubmed publisher
Bence M, Jankovics F, Lukacsovich T, Erdelyi M. Combining the auxin-inducible degradation system with CRISPR/Cas9-based genome editing for the conditional depletion of endogenous Drosophila melanogaster proteins. FEBS J. 2017;284:1056-1069 pubmed publisher
Karam J, Parikh R, Nayak D, Rosenkranz D, Gangaraju V. Co-chaperone Hsp70/Hsp90-organizing protein (Hop) is required for transposon silencing and Piwi-interacting RNA (piRNA) biogenesis. J Biol Chem. 2017;292:6039-6046 pubmed publisher
Chiang A, Yang H, Yamashita Y. spict, a cyst cell-specific gene, regulates starvation-induced spermatogonial cell death in the Drosophila testis. Sci Rep. 2017;7:40245 pubmed publisher
Marie P, Ronsseray S, Boivin A. From Embryo to Adult: piRNA-Mediated Silencing throughout Germline Development in Drosophila. G3 (Bethesda). 2017;7:505-516 pubmed publisher
Zheng J, Gao M, Huynh N, Tindell S, Vo H, McDonald W, et al. In vivo mapping of a dynamic ribonucleoprotein granule interactome in early Drosophila embryos. FEBS Open Bio. 2016;6:1248-1256 pubmed publisher
Chen C, Inaba M, Venkei Z, Yamashita Y. Klp10A, a stem cell centrosome-enriched kinesin, balances asymmetries in Drosophila male germline stem cell division. elife. 2016;5: pubmed publisher
Wissel S, Kieser A, Yasugi T, Duchek P, Roitinger E, Gokcezade J, et al. A Combination of CRISPR/Cas9 and Standardized RNAi as a Versatile Platform for the Characterization of Gene Function. G3 (Bethesda). 2016;6:2467-78 pubmed publisher
Ables E, Hwang G, Finger D, Hinnant T, Drummond Barbosa D. A Genetic Mosaic Screen Reveals Ecdysone-Responsive Genes Regulating Drosophila Oogenesis. G3 (Bethesda). 2016;6:2629-42 pubmed publisher
Bodnar A, Coffman J. Maintenance of somatic tissue regeneration with age in short- and long-lived species of sea urchins. Aging Cell. 2016;15:778-87 pubmed publisher
Lo P, Huang Y, Poulton J, Leake N, Palmer W, Vera D, et al. RNA helicase Belle/DDX3 regulates transgene expression in Drosophila. Dev Biol. 2016;412:57-70 pubmed publisher
Ong C, Lee Q, Cai Y, Liu X, Ding J, Yung L, et al. Silver nanoparticles disrupt germline stem cell maintenance in the Drosophila testis. Sci Rep. 2016;6:20632 pubmed publisher
Sun J, Wei H, Xu J, Chang J, Yang Z, Ren X, et al. Histone H1-mediated epigenetic regulation controls germline stem cell self-renewal by modulating H4K16 acetylation. Nat Commun. 2015;6:8856 pubmed publisher
Tarayrah L, Li Y, Gan Q, Chen X. Epigenetic regulator Lid maintains germline stem cells through regulating JAK-STAT signaling pathway activity. Biol Open. 2015;4:1518-27 pubmed publisher
Reinardy H, Emerson C, Manley J, Bodnar A. Tissue regeneration and biomineralization in sea urchins: role of Notch signaling and presence of stem cell markers. PLoS ONE. 2015;10:e0133860 pubmed publisher
Reilly E, Changela N, Naryshkina T, Deshpande G, Steward R. Discs large 5, an Essential Gene in Drosophila, Regulates Egg Chamber Organization. G3 (Bethesda). 2015;5:943-52 pubmed publisher
Matsuoka S, Gupta S, Suzuki E, Hiromi Y, Asaoka M. gone early, a novel germline factor, ensures the proper size of the stem cell precursor pool in the Drosophila ovary. PLoS ONE. 2014;9:e113423 pubmed publisher
Yu J, Wu H, Wen Y, Liu Y, Zhou T, Ni B, et al. Identification of seven genes essential for male fertility through a genome-wide association study of non-obstructive azoospermia and RNA interference-mediated large-scale functional screening in Drosophila. Hum Mol Genet. 2015;24:1493-503 pubmed publisher
Pan L, Wang S, Lu T, Weng C, Song X, Park J, et al. Protein competition switches the function of COP9 from self-renewal to differentiation. Nature. 2014;514:233-6 pubmed publisher
Spracklen A, Fagan T, Lovander K, Tootle T. The pros and cons of common actin labeling tools for visualizing actin dynamics during Drosophila oogenesis. Dev Biol. 2014;393:209-226 pubmed publisher
Elliott T, Townsley F, Bianco A, Ernst R, Sachdeva A, Elsässer S, et al. Proteome labeling and protein identification in specific tissues and at specific developmental stages in an animal. Nat Biotechnol. 2014;32:465-72 pubmed publisher
Barton L, Wilmington S, Martin M, Skopec H, Lovander K, Pinto B, et al. Unique and shared functions of nuclear lamina LEM domain proteins in Drosophila. Genetics. 2014;197:653-65 pubmed publisher
Satyaki P, Cuykendall T, Wei K, Brideau N, Kwak H, Aruna S, et al. The Hmr and Lhr hybrid incompatibility genes suppress a broad range of heterochromatic repeats. PLoS Genet. 2014;10:e1004240 pubmed publisher
Eliazer S, Palacios V, Wang Z, Kollipara R, Kittler R, Buszczak M. Lsd1 restricts the number of germline stem cells by regulating multiple targets in escort cells. PLoS Genet. 2014;10:e1004200 pubmed publisher
Tsai Y, Chiang W, Liou W, Lee W, Chang Y, Wang P, et al. Endophilin B is required for the Drosophila oocyte to endocytose yolk downstream of Oskar. Development. 2014;141:563-73 pubmed publisher
Joyce E, Apostolopoulos N, Beliveau B, Wu C. Germline progenitors escape the widespread phenomenon of homolog pairing during Drosophila development. PLoS Genet. 2013;9:e1004013 pubmed publisher
Jin Z, Flynt A, Lai E. Drosophila piwi mutants exhibit germline stem cell tumors that are sustained by elevated Dpp signaling. Curr Biol. 2013;23:1442-8 pubmed publisher
Yan R, McKee B. The cohesion protein SOLO associates with SMC1 and is required for synapsis, recombination, homolog bias and cohesion and pairing of centromeres in Drosophila Meiosis. PLoS Genet. 2013;9:e1003637 pubmed publisher
Renault A. vasa is expressed in somatic cells of the embryonic gonad in a sex-specific manner in Drosophila melanogaster. Biol Open. 2012;1:1043-8 pubmed publisher
Kelleher E, Edelman N, Barbash D. Drosophila interspecific hybrids phenocopy piRNA-pathway mutants. PLoS Biol. 2012;10:e1001428 pubmed publisher
Nagel A, Szawinski J, Fischer P, Maier D, Wech I, Preiss A. Dorso-ventral axis formation of the Drosophila oocyte requires Cyclin G. Hereditas. 2012;149:186-96 pubmed publisher
Lim J, Fuller M. Somatic cell lineage is required for differentiation and not maintenance of germline stem cells in Drosophila testes. Proc Natl Acad Sci U S A. 2012;109:18477-81 pubmed publisher
Groen C, Spracklen A, Fagan T, Tootle T. Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling. Mol Biol Cell. 2012;23:4567-78 pubmed publisher
Nagel A, Fischer P, Szawinski J, La Rosa M, Preiss A. Cyclin G is involved in meiotic recombination repair in Drosophila melanogaster. J Cell Sci. 2012;125:5555-63 pubmed publisher
Hohl A, Thompson M, Soshnev A, Wu J, Morris J, Hsieh T, et al. Restoration of topoisomerase 2 function by complementation of defective monomers in Drosophila. Genetics. 2012;192:843-56 pubmed publisher
Rojas Ríos P, Guerrero I, González Reyes A. Cytoneme-mediated delivery of hedgehog regulates the expression of bone morphogenetic proteins to maintain germline stem cells in Drosophila. PLoS Biol. 2012;10:e1001298 pubmed publisher
Roth T, Chiang C, Inaba M, Yuan H, Salzmann V, Roth C, et al. Centrosome misorientation mediates slowing of the cell cycle under limited nutrient conditions in Drosophila male germline stem cells. Mol Biol Cell. 2012;23:1524-32 pubmed publisher
Cash A, Andrews J. Fine scale analysis of gene expression in Drosophila melanogaster gonads reveals Programmed cell death 4 promotes the differentiation of female germline stem cells. BMC Dev Biol. 2012;12:4 pubmed publisher
Wang X, Pan L, Wang S, Zhou J, McDowell W, Park J, et al. Histone H3K9 trimethylase Eggless controls germline stem cell maintenance and differentiation. PLoS Genet. 2011;7:e1002426 pubmed publisher
Song Y, Lu B. Regulation of cell growth by Notch signaling and its differential requirement in normal vs. tumor-forming stem cells in Drosophila. Genes Dev. 2011;25:2644-58 pubmed publisher
Kibanov M, Egorova K, Ryazansky S, Sokolova O, Kotov A, Olenkina O, et al. A novel organelle, the piNG-body, in the nuage of Drosophila male germ cells is associated with piRNA-mediated gene silencing. Mol Biol Cell. 2011;22:3410-9 pubmed publisher
Hayashi Y, Kobayashi S, Nakato H. Drosophila glypicans regulate the germline stem cell niche. J Cell Biol. 2009;187:473-80 pubmed publisher
Gracheva E, Dus M, Elgin S. Drosophila RISC component VIG and its homolog Vig2 impact heterochromatin formation. PLoS ONE. 2009;4:e6182 pubmed publisher
Aruna S, Flores H, Barbash D. Reduced fertility of Drosophila melanogaster hybrid male rescue (Hmr) mutant females is partially complemented by Hmr orthologs from sibling species. Genetics. 2009;181:1437-50 pubmed publisher
product information
Internal ID :
2284
Name :
anti-vasa
Depositor Name :
Spradling, A.C. / Williams, D.
Depositor Institution :
Carnegie Institute of Washington
Date Deposited :
1/9/07
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
vasa
Antigen Species :
Drosophila
Host Species :
rat
Isotype :
RIgM, kappa light chain
Isotype for catalog (legacy) :
IgM, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
zebrafish, fish
Initial Publication Pubmed ID :
19153254
Collections :
Cell markers,Drosophila antigens,Enzymes
Search Keywords :
Spradling, A.C. / Williams, D., vasa, Drosophila, RIgM, kappa light chain, Drosophila, vas, CG46283, AB_760351, monoclonal, Drosophila antigens/Cell markers/Enzymes, Immunohistochemistry/Immunofluorescence/Western Blot
Antigen Molecular Weight :
Predicted: 72 kDa; Apparent 72 kDa
Gene :
vas
Alternate Gene Name(s) :
CG46283
Uniprot ID :
P09052
Antibody Registry ID :
AB_760351
Immunogen :
Recombinant fusion protein GST-vasa
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
23213382
Immunofluorescence Pubmed IDs :
22190460 23213382 24385920 25361961 22216012 19153254 26900887 24651406 25119050 23891114 24401369 23121330 28809158 30389853
Western Blot Pubmed IDs :
23051736 24995797 22976300
Additional Information :
Vasa is a germline specific marker
DSHB Growth Medium :
Iscove's
References (legacy) :
Biol. Open. 1(10), 1043-1048.; doi: 10.1016/j.ydbio.2014.06.022; doi: 10.1091/mbc.E12-05-0417; doi: 10.1101/gad.171959.111
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US