product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Eyeless protein, linker region
catalog :
anti-eyeless
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
anti-eyeless
reactivity :
Drosophila melanogaster
application :
immunocytochemistry
citations: 19
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; Drosophila melanogaster; 1:50; loading ...; fig 1e
Abdusselamoglu M, Eroglu E, Burkard T, Knoblich J. The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop. elife. 2019;8: pubmed publisher
Ramaekers A, Claeys A, Kapun M, Mouchel Vielh E, Potier D, Weinberger S, et al. Altering the Temporal Regulation of One Transcription Factor Drives Evolutionary Trade-Offs between Head Sensory Organs. Dev Cell. 2019;50:780-792.e7 pubmed publisher
Widmer Y, Fritsch C, Jungo M, Almeida S, Egger B, Sprecher S. Multiple neurons encode CrebB dependent appetitive long-term memory in the mushroom body circuit. elife. 2018;7: pubmed publisher
Zhu J, Ordway A, Weber L, Buddika K, Kumar J. Polycomb group (PcG) proteins and Pax6 cooperate to inhibit in vivo reprogramming of the developing Drosophila eye. Development. 2018;145: pubmed publisher
Erclik T, Li X, Courgeon M, Bertet C, Chen Z, Baumert R, et al. Integration of temporal and spatial patterning generates neural diversity. Nature. 2017;541:365-370 pubmed publisher
Bertet C, Li X, Erclik T, Cavey M, Wells B, Desplan C. Temporal patterning of neuroblasts controls Notch-mediated cell survival through regulation of Hid or Reaper. Cell. 2014;158:1173-1186 pubmed publisher
Anderson A, Weasner B, Weasner B, Kumar J. The Drosophila Wilms׳ Tumor 1-Associating Protein (WTAP) homolog is required for eye development. Dev Biol. 2014;390:170-80 pubmed publisher
Blaquiere J, Lee W, Verheyen E. Hipk promotes photoreceptor differentiation through the repression of Twin of eyeless and Eyeless expression. Dev Biol. 2014;390:14-25 pubmed publisher
Eroglu E, Burkard T, Jiang Y, Saini N, Homem C, Reichert H, et al. SWI/SNF complex prevents lineage reversion and induces temporal patterning in neural stem cells. Cell. 2014;156:1259-1273 pubmed publisher
Turkel N, Sahota V, Bolden J, Goulding K, Doggett K, Willoughby L, et al. The BTB-zinc finger transcription factor abrupt acts as an epithelial oncogene in Drosophila melanogaster through maintaining a progenitor-like cell state. PLoS Genet. 2013;9:e1003627 pubmed publisher
Li X, Erclik T, Bertet C, Chen Z, Voutev R, Venkatesh S, et al. Temporal patterning of Drosophila medulla neuroblasts controls neural fates. Nature. 2013;498:456-62 pubmed publisher
Suzuki T, Kaido M, Takayama R, Sato M. A temporal mechanism that produces neuronal diversity in the Drosophila visual center. Dev Biol. 2013;380:12-24 pubmed publisher
Lanet E, Gould A, Maurange C. Protection of neuronal diversity at the expense of neuronal numbers during nutrient restriction in the Drosophila visual system. Cell Rep. 2013;3:587-94 pubmed publisher
Anderson A, Weasner B, Weasner B, Kumar J. Dual transcriptional activities of SIX proteins define their roles in normal and ectopic eye development. Development. 2012;139:991-1000 pubmed publisher
Okamoto N, Nishimori Y, Nishimura T. Conserved role for the Dachshund protein with Drosophila Pax6 homolog Eyeless in insulin expression. Proc Natl Acad Sci U S A. 2012;109:2406-11 pubmed publisher
Morante J, Erclik T, Desplan C. Cell migration in Drosophila optic lobe neurons is controlled by eyeless/Pax6. Development. 2011;138:687-93 pubmed publisher
Brockmann A, Domínguez Cejudo M, Amore G, Casares F. Regulation of ocellar specification and size by twin of eyeless and homothorax. Dev Dyn. 2011;240:75-85 pubmed publisher
Lopes C, Casares F. hth maintains the pool of eye progenitors and its downregulation by Dpp and Hh couples retinal fate acquisition with cell cycle exit. Dev Biol. 2010;339:78-88 pubmed publisher
Clements J, Hens K, Francis C, Schellens A, Callaerts P. Conserved role for the Drosophila Pax6 homolog Eyeless in differentiation and function of insulin-producing neurons. Proc Natl Acad Sci U S A. 2008;105:16183-8 pubmed publisher
product information
Internal ID :
6371
Name :
anti-eyeless
Depositor Name :
Callaerts, P.
Depositor Institution :
VIB Lab of Behavioral & Developmental Genetics, KU Leuven
Date Deposited :
8/12/09
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Eyeless protein, linker region
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Drosophila melanogaster
Species Tested (legacy) :
Drosophila melanogaster
Initial Publication Pubmed ID :
18852455
Depositor Notes (Special Instructions) :
The depositor indicates this antibody works in western blot applications.
Collections :
Drosophila antigens,Neurodevelopment,Transcription factors
Search Keywords :
Patrick Callaerts, Eyeless protein, linker region, Drosophila, MIgG2a, Drosophila melanogaster, ey, Dmel CG1464; DPax-6; EYEL; OK107; Pax6, AB_2253542, monoclonal
Antigen Molecular Weight :
Predicted: 47-48kDa
Gene :
ey
Alternate Gene Name(s) :
Dmel CG1464; DPax-6; EYEL; OK107; Pax6
Uniprot ID :
O18381
Antibody Registry ID :
AB_2253542
Synonyms (Alternate Clone Names) :
anti-Ey
Immunogen :
Recombinant protein fragment (residues 226-470 (the linker region) of the Eyeless protein, transcript variant D)
Immunogen Sequence :
Residues 226-470 of transcript variant D correspond to 185-429 on the canonical Larval isoform [Uniprot O18381]
Clonality :
Monoclonal
Myeloma Strain :
SP2OAg14
Epitope Mapped :
Yes
Epitope Location or Sequence :
a.a. 185-429
Epitope Map PubMed ID :
18852455
Recommended Applications :
Immunofluorescence
Immunoblotting (legacy) :
yes
Immunofluorescence Pubmed IDs :
18852455 24631217 21104743 22308399 21208993 23783517 24630726 20036228 22318629 23665475 23874226 23478023 25171415 29530880
Pubmed IDs :
24690230
Depositor Growth Medium :
Iscove's
Additional Information :
The epitope is not in the Paired DNA binding domain.
DSHB Growth Medium :
Iscove's
References (legacy) :
Proc. Natl. Acad. Sci. USA 105, 16183-16188.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US