product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Dorsal, embryonic polarity protein
catalog :
anti-dorsal 7A4
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
anti-dorsal 7A4
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 21
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; fruit fly; fig 2
Liu D, Shaukat Z, Saint R, Gregory S. Chromosomal instability triggers cell death via local signalling through the innate immune receptor Toll. Oncotarget. 2015;6:38552-65 pubmed publisher
Wisotzkey R, Quijano J, Stinchfield M, Newfeld S. New gene evolution in the bonus-TIF1-?/TRIM33 family impacted the architecture of the vertebrate dorsal-ventral patterning network. Mol Biol Evol. 2014;31:2309-21 pubmed publisher
Ming M, Obata F, Kuranaga E, Miura M. Persephone/Spätzle pathogen sensors mediate the activation of Toll receptor signaling in response to endogenous danger signals in apoptosis-deficient Drosophila. J Biol Chem. 2014;289:7558-68 pubmed publisher
Zhou B, Yun E, Ray L, You J, Ip Y, Lin X. Retromer promotes immune quiescence by suppressing Spätzle-Toll pathway in Drosophila. J Cell Physiol. 2014;229:512-520 pubmed publisher
Gueguen G, Kalamarz M, Ramroop J, Uribe J, Govind S. Polydnaviral ankyrin proteins aid parasitic wasp survival by coordinate and selective inhibition of hematopoietic and immune NF-kappa B signaling in insect hosts. PLoS Pathog. 2013;9:e1003580 pubmed publisher
McIlroy G, Földi I, Aurikko J, Wentzell J, Lim M, Fenton J, et al. Toll-6 and Toll-7 function as neurotrophin receptors in the Drosophila melanogaster CNS. Nat Neurosci. 2013;16:1248-56 pubmed publisher
Fontenele M, Lim B, Oliveira D, Buffolo M, Perlman D, Schupbach T, et al. Calpain A modulates Toll responses by limited Cactus/IκB proteolysis. Mol Biol Cell. 2013;24:2966-80 pubmed publisher
Tremmel D, Resad S, Little C, Wesley C. Notch and PKC are involved in formation of the lateral region of the dorso-ventral axis in Drosophila embryos. PLoS ONE. 2013;8:e67789 pubmed publisher
Dunipace L, Ozdemir A, Stathopoulos A. Complex interactions between cis-regulatory modules in native conformation are critical for Drosophila snail expression. Development. 2011;138:4075-84 pubmed publisher
Paddibhatla I, Lee M, Kalamarz M, Ferrarese R, Govind S. Role for sumoylation in systemic inflammation and immune homeostasis in Drosophila larvae. PLoS Pathog. 2010;6:e1001234 pubmed publisher
Liberman L, Reeves G, Stathopoulos A. Quantitative imaging of the Dorsal nuclear gradient reveals limitations to threshold-dependent patterning in Drosophila. Proc Natl Acad Sci U S A. 2009;106:22317-22 pubmed publisher
Kanodia J, Rikhy R, Kim Y, Lund V, DeLotto R, Lippincott Schwartz J, et al. Dynamics of the Dorsal morphogen gradient. Proc Natl Acad Sci U S A. 2009;106:21707-12 pubmed publisher
Mavrakis M, Rikhy R, Lippincott Schwartz J. Plasma membrane polarity and compartmentalization are established before cellularization in the fly embryo. Dev Cell. 2009;16:93-104 pubmed publisher
Ching W, Hang H, Nusse R. Lipid-independent secretion of a Drosophila Wnt protein. J Biol Chem. 2008;283:17092-8 pubmed publisher
Bosveld F, van Hoek S, Sibon O. Establishment of cell fate during early Drosophila embryogenesis requires transcriptional Mediator subunit dMED31. Dev Biol. 2008;313:802-13 pubmed
Mindorff E, O Keefe D, Labbe A, Yang J, Ou Y, Yoshikawa S, et al. A gain-of-function screen for genes that influence axon guidance identifies the NF-kappaB protein dorsal and reveals a requirement for the kinase Pelle in Drosophila photoreceptor axon targeting. Genetics. 2007;176:2247-63 pubmed
Carneiro K, Fontenele M, Negreiros E, Lopes E, Bier E, Araujo H. Graded maternal short gastrulation protein contributes to embryonic dorsal-ventral patterning by delayed induction. Dev Biol. 2006;296:203-18 pubmed
Ganguly A, Jiang J, Ip Y. Drosophila WntD is a target and an inhibitor of the Dorsal/Twist/Snail network in the gastrulating embryo. Development. 2005;132:3419-29 pubmed
Bettencourt R, Asha H, Dearolf C, Ip Y. Hemolymph-dependent and -independent responses in Drosophila immune tissue. J Cell Biochem. 2004;92:849-63 pubmed
Govind S, Drier E, Huang L, Steward R. Regulated nuclear import of the Drosophila rel protein dorsal: structure-function analysis. Mol Cell Biol. 1996;16:1103-14 pubmed
Whalen A, Steward R. Dissociation of the dorsal-cactus complex and phosphorylation of the dorsal protein correlate with the nuclear localization of dorsal. J Cell Biol. 1993;123:523-34 pubmed
product information
Internal ID :
2362
Name :
anti-dorsal 7A4
Depositor Name :
Steward, R.
Depositor Institution :
Rutgers University
Date Deposited :
3/20/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Dorsal, embryonic polarity protein
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
8227123
Depositor Notes (Special Instructions) :
MAb 7a4 recognizes the C-terminal region of dorsal (404-677) of isoform A (D). This sequence is not shared with isoform C (F).
Collections :
Drosophila antigens,Transcription factors
Search Keywords :
Steward, Ruth, Dorsal, embryonic polarity protein, Drosophila, MIgG1, Drosophila, dl, Dmel CG6667; NFkappaB; , AB_528204, monoclonal
Antigen Molecular Weight :
Predicted: 75kDa; Apparent: 85 to 90kDa
Gene :
dl
Alternate Gene Name(s) :
Dmel CG6667; NFkappaB;
Uniprot ID :
E1JHK3
Antibody Registry ID :
AB_528204
Synonyms (Alternate Clone Names) :
anti-dorsal; anti-dl; 7A4-39
Immunogen :
Recombinant fusion protein (isoform A or D).
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
SP2/0
Epitope Mapped :
Yes
Epitope Location or Sequence :
a.a. 404-677
Epitope Map PubMed ID :
8227123
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
17603113 23861806
Immunofluorescence Pubmed IDs :
18083158 16781701 19996178 24492611 15211580 19154721 24343480 17603113 15987775 21203476 24881051 23864715 20018754 18430724 24009508 8622655
Western Blot Pubmed IDs :
23892553 23861806 23864715 8622655
Immunoprecipitation Pubmed IDs :
8227123 23864715 8622655
Pubmed IDs :
21813571
Depositor Growth Medium :
RPMI
Additional Information :
Dorsal is the homolog of the mammalian NF-KappaB (homology to NF-KappaB in the N-terminal region of dorsal not recognized by the mAb 7A4).
DSHB Growth Medium :
RPMI
References (legacy) :
J. Cell Biol. 123(3), 523-534.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US