product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Dorsal, embryonic polarity protein
catalog :
anti-dorsal 7A4
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
anti-dorsal 7A4
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
citations: 21
Published Application/Species/Sample/Dilution | Reference |
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Bosveld F, van Hoek S, Sibon O. Establishment of cell fate during early Drosophila embryogenesis requires transcriptional Mediator subunit dMED31. Dev Biol. 2008;313:802-13 pubmed
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Mindorff E, O Keefe D, Labbe A, Yang J, Ou Y, Yoshikawa S, et al. A gain-of-function screen for genes that influence axon guidance identifies the NF-kappaB protein dorsal and reveals a requirement for the kinase Pelle in Drosophila photoreceptor axon targeting. Genetics. 2007;176:2247-63 pubmed
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Carneiro K, Fontenele M, Negreiros E, Lopes E, Bier E, Araujo H. Graded maternal short gastrulation protein contributes to embryonic dorsal-ventral patterning by delayed induction. Dev Biol. 2006;296:203-18 pubmed
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Ganguly A, Jiang J, Ip Y. Drosophila WntD is a target and an inhibitor of the Dorsal/Twist/Snail network in the gastrulating embryo. Development. 2005;132:3419-29 pubmed
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Bettencourt R, Asha H, Dearolf C, Ip Y. Hemolymph-dependent and -independent responses in Drosophila immune tissue. J Cell Biochem. 2004;92:849-63 pubmed
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Govind S, Drier E, Huang L, Steward R. Regulated nuclear import of the Drosophila rel protein dorsal: structure-function analysis. Mol Cell Biol. 1996;16:1103-14 pubmed
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Whalen A, Steward R. Dissociation of the dorsal-cactus complex and phosphorylation of the dorsal protein correlate with the nuclear localization of dorsal. J Cell Biol. 1993;123:523-34 pubmed
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product information
Internal ID :
2362
Name :
anti-dorsal 7A4
Depositor Name :
Steward, R.
Depositor Institution :
Rutgers University
Date Deposited :
3/20/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Dorsal, embryonic polarity protein
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
8227123
Depositor Notes (Special Instructions) :
MAb 7a4 recognizes the C-terminal region of dorsal (404-677) of isoform A (D). This sequence is not shared with isoform C (F).
Collections :
Drosophila antigens,Transcription factors
Search Keywords :
Steward, Ruth, Dorsal, embryonic polarity protein, Drosophila, MIgG1, Drosophila, dl, Dmel CG6667; NFkappaB; , AB_528204, monoclonal
Antigen Molecular Weight :
Predicted: 75kDa; Apparent: 85 to 90kDa
Gene :
dl
Alternate Gene Name(s) :
Dmel CG6667; NFkappaB;
Uniprot ID :
E1JHK3
Antibody Registry ID :
AB_528204
Synonyms (Alternate Clone Names) :
anti-dorsal; anti-dl; 7A4-39
Immunogen :
Recombinant fusion protein (isoform A or D).
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
SP2/0
Epitope Mapped :
Yes
Epitope Location or Sequence :
a.a. 404-677
Epitope Map PubMed ID :
8227123
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
17603113 23861806
Immunofluorescence Pubmed IDs :
18083158 16781701 19996178 24492611 15211580 19154721 24343480 17603113 15987775 21203476 24881051 23864715 20018754 18430724 24009508 8622655
Western Blot Pubmed IDs :
23892553 23861806 23864715 8622655
Immunoprecipitation Pubmed IDs :
8227123 23864715 8622655
Pubmed IDs :
21813571
Depositor Growth Medium :
RPMI
Additional Information :
Dorsal is the homolog of the mammalian NF-KappaB (homology to NF-KappaB in the N-terminal region of dorsal not recognized by the mAb 7A4).
DSHB Growth Medium :
RPMI
References (legacy) :
J. Cell Biol. 123(3), 523-534.
more info or order :
company information
Developmental Studies Hybridoma Bank