AP-3 (delta subunit) | Developmental Studies Hybridoma Bank anti-delta SA4 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

AP-3 (delta subunit)

catalog :

anti-delta SA4

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

anti-delta SA4

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Reference
Vassilopoulos S, Gentil C, Laine J, Buclez P, Franck A, Ferry A, et al. Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization. J Cell Biol. 2014;205:377-93 pubmed publisher
Zlatic S, Grossniklaus E, Ryder P, Salazar G, Mattheyses A, Peden A, et al. Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis. Mol Biol Cell. 2013;24:2378-88 pubmed publisher
Kural C, Tacheva Grigorova S, Boulant S, Cocucci E, Baust T, Duarte D, et al. Dynamics of intracellular clathrin/AP1- and clathrin/AP3-containing carriers. Cell Rep. 2012;2:1111-9 pubmed publisher
Kent H, Evans P, SchÃ¤fer I, Gray S, Sanderson C, Luzio J, et al. Structural basis of the intracellular sorting of the SNARE VAMP7 by the AP3 adaptor complex. Dev Cell. 2012;22:979-88 pubmed publisher
Newell Litwa K, Chintala S, Jenkins S, Pare J, McGaha L, Smith Y, et al. Hermansky-Pudlak protein complexes, AP-3 and BLOC-1, differentially regulate presynaptic composition in the striatum and hippocampus. J Neurosci. 2010;30:820-31 pubmed publisher
Lefrancois S, McCormick P. The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes. Traffic. 2007;8:1440-51 pubmed
Xiao J, Dai R, Negyessy L, Bergson C. Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis. J Biol Chem. 2006;281:15182-93 pubmed
Salazar G, Craige B, Wainer B, Guo J, De Camilli P, Faundez V. Phosphatidylinositol-4-kinase type II alpha is a component of adaptor protein-3-derived vesicles. Mol Biol Cell. 2005;16:3692-704 pubmed
Seong E, Wainer B, Hughes E, Saunders T, Burmeister M, Faundez V. Genetic analysis of the neuronal and ubiquitous AP-3 adaptor complexes reveals divergent functions in brain. Mol Biol Cell. 2005;16:128-40 pubmed
Ihrke G, Kyttälä A, Russell M, Rous B, Luzio J. Differential use of two AP-3-mediated pathways by lysosomal membrane proteins. Traffic. 2004;5:946-62 pubmed
Peden A, Oorschot V, Hesser B, Austin C, Scheller R, Klumperman J. Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins. J Cell Biol. 2004;164:1065-76 pubmed

product information

Internal ID :

1440

Name :

anti-delta SA4

Depositor Name :

Peden, A.A.

Depositor Institution :

Genentech Inc., 1 DNA Way, South San Francisco

Date Deposited :

1/28/03

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

AP-3 (delta subunit)

Antigen Species :

human

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Human,Mammal,Mouse

Species Tested (legacy) :

all mammals tested

Initial Publication Pubmed ID :

15051738

Depositor Notes (Special Instructions) :

Works on all mammalian cell lines tested. Specific to delta adaptin by western blot and immunofluorescence.

Collections :

Cell signaling,Cytoskeleton,Human,Neurodevelopment

Search Keywords :

Peden, A.A., AP-3 (delta subunit), human, MIgG1, kappa light chain, mammal/mouse/human, AP3D1, ADTD, HPS10, hBLVR, AB_2056641, monoclonal, Cell signaling/Cytoskeletal elements/Human/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: multiple isoforms between 11 and 130 kDa; Apparent: 160 kDa

Gene :

AP3D1

Alternate Gene Name(s) :

ADTD, HPS10, hBLVR

Uniprot ID :

O14617

Antibody Registry ID :

AB_2056641

Synonyms (Alternate Clone Names) :

SA4

Immunogen :

Recombinant GST/delta adaptin (aa 608-800) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Yes

Epitope Location or Sequence :

Hinge region (aa 770-800 of isoform 1: O14617-1)

Epitope Map PubMed ID :

15051738 23761069

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

20089890

Immunofluorescence Pubmed IDs :

15051738 22521722 23103167 15522097 15537701 17666033 31353312

Western Blot Pubmed IDs :

15051738 16595675 24798732 23761069 15537701 31353312

Immunoprecipitation Pubmed IDs :

22521722 23761069 15537701 15944223

Additional Information :

The AP-3 complex subunit delta-1 is also known as delta adaptin.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 164, 1065-1076.

more info or order :

Developmental Studies Hybridoma Bank product webpage