product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
cactus
catalog :
anti-cactus 3H12
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
anti-cactus 3H12
reactivity :
fruit fly
application :
western blot, immunocytochemistry, immunoprecipitation
citations: 9
Reference
Loya C, McNeill E, Bao H, Zhang B, Van Vactor D. miR-8 controls synapse structure by repression of the actin regulator enabled. Development. 2014;141:1864-74 pubmed publisher
McIlroy G, Földi I, Aurikko J, Wentzell J, Lim M, Fenton J, et al. Toll-6 and Toll-7 function as neurotrophin receptors in the Drosophila melanogaster CNS. Nat Neurosci. 2013;16:1248-56 pubmed publisher
Fontenele M, Lim B, Oliveira D, Buffolo M, Perlman D, Schupbach T, et al. Calpain A modulates Toll responses by limited Cactus/IκB proteolysis. Mol Biol Cell. 2013;24:2966-80 pubmed publisher
Tremmel D, Resad S, Little C, Wesley C. Notch and PKC are involved in formation of the lateral region of the dorso-ventral axis in Drosophila embryos. PLoS ONE. 2013;8:e67789 pubmed publisher
Paddibhatla I, Lee M, Kalamarz M, Ferrarese R, Govind S. Role for sumoylation in systemic inflammation and immune homeostasis in Drosophila larvae. PLoS Pathog. 2010;6:e1001234 pubmed publisher
Fontenele M, Carneiro K, Agrellos R, Oliveira D, Oliveira Silva A, Vieira V, et al. The Ca2+-dependent protease Calpain A regulates Cactus/I kappaB levels during Drosophila development in response to maternal Dpp signals. Mech Dev. 2009;126:737-51 pubmed publisher
Mindorff E, O Keefe D, Labbe A, Yang J, Ou Y, Yoshikawa S, et al. A gain-of-function screen for genes that influence axon guidance identifies the NF-kappaB protein dorsal and reveals a requirement for the kinase Pelle in Drosophila photoreceptor axon targeting. Genetics. 2007;176:2247-63 pubmed
Govind S, Drier E, Huang L, Steward R. Regulated nuclear import of the Drosophila rel protein dorsal: structure-function analysis. Mol Cell Biol. 1996;16:1103-14 pubmed
Whalen A, Steward R. Dissociation of the dorsal-cactus complex and phosphorylation of the dorsal protein correlate with the nuclear localization of dorsal. J Cell Biol. 1993;123:523-34 pubmed
product information
Internal ID :
2356
Name :
anti-cactus 3H12
Depositor Name :
Steward, R.
Depositor Institution :
Waksman Institute, Rutgers University
Date Deposited :
3/20/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
cactus
Antigen Species :
Drosophila melanogaster
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
8227123
Depositor Notes (Special Instructions) :
Works for immunoprecipitation, but not very well.
Collections :
Drosophila antigens,Transcription factors
Search Keywords :
Steward, R., cactus, Drosophila melanogaster, MIgG1, Drosophila, cact, CG5848, cac, dip6, IkappaB, AB_528109, monoclonal, Drosophila antigens/Transcription factors, Immunofluorescence/Western blot/Immunoprecipitation
Antigen Molecular Weight :
Predicted: 52 and 54 kDa; Apparent: 69, 71, and 72 kDa
Gene :
cact
Alternate Gene Name(s) :
CG5848, cac, dip6, IkappaB
Uniprot ID :
Q03017
Antibody Registry ID :
AB_528109
Synonyms (Alternate Clone Names) :
3H12-29
Immunogen :
Recombinant GST/cactus fusion protein
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
SP2/0
Epitope Mapped :
Yes
Epitope Location or Sequence :
C-terminal (aa 262-458)
Epitope Map PubMed ID :
8227123 19442719
Recommended Applications :
Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunofluorescence Pubmed IDs :
8227123 19442719 21203476 17603113 23864715 23861806 24718988
Western Blot Pubmed IDs :
8227123 19442719 8622655 23864715 23861806 23892553
Immunoprecipitation Pubmed IDs :
8227123
Additional Information :
Interaction of dorsal with cactus appears to make the 3H12 epitope inaccessible. 3H12 recognizes all isoforms.
DSHB Growth Medium :
RPMI
References (legacy) :
J. Cell Biol. 123(3), 523-534.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US