product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
cactus
catalog :
anti-cactus 3H12
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
anti-cactus 3H12
reactivity :
fruit fly
application :
western blot, immunocytochemistry, immunoprecipitation
more info or order :
citations: 9
Reference |
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Mindorff E, O Keefe D, Labbe A, Yang J, Ou Y, Yoshikawa S, et al. A gain-of-function screen for genes that influence axon guidance identifies the NF-kappaB protein dorsal and reveals a requirement for the kinase Pelle in Drosophila photoreceptor axon targeting. Genetics. 2007;176:2247-63 pubmed
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Govind S, Drier E, Huang L, Steward R. Regulated nuclear import of the Drosophila rel protein dorsal: structure-function analysis. Mol Cell Biol. 1996;16:1103-14 pubmed
|
Whalen A, Steward R. Dissociation of the dorsal-cactus complex and phosphorylation of the dorsal protein correlate with the nuclear localization of dorsal. J Cell Biol. 1993;123:523-34 pubmed
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product information
Internal ID :
2356
Name :
anti-cactus 3H12
Depositor Name :
Steward, R.
Depositor Institution :
Waksman Institute, Rutgers University
Date Deposited :
3/20/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
cactus
Antigen Species :
Drosophila melanogaster
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
8227123
Depositor Notes (Special Instructions) :
Works for immunoprecipitation, but not very well.
Collections :
Drosophila antigens,Transcription factors
Search Keywords :
Steward, R., cactus, Drosophila melanogaster, MIgG1, Drosophila, cact, CG5848, cac, dip6, IkappaB, AB_528109, monoclonal, Drosophila antigens/Transcription factors, Immunofluorescence/Western blot/Immunoprecipitation
Antigen Molecular Weight :
Predicted: 52 and 54 kDa; Apparent: 69, 71, and 72 kDa
Gene :
cact
Alternate Gene Name(s) :
CG5848, cac, dip6, IkappaB
Uniprot ID :
Q03017
Antibody Registry ID :
AB_528109
Synonyms (Alternate Clone Names) :
3H12-29
Immunogen :
Recombinant GST/cactus fusion protein
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
SP2/0
Epitope Mapped :
Yes
Epitope Location or Sequence :
C-terminal (aa 262-458)
Epitope Map PubMed ID :
8227123 19442719
Recommended Applications :
Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunofluorescence Pubmed IDs :
8227123 19442719 21203476 17603113 23864715 23861806 24718988
Western Blot Pubmed IDs :
8227123 19442719 8622655 23864715 23861806 23892553
Immunoprecipitation Pubmed IDs :
8227123
Additional Information :
Interaction of dorsal with cactus appears to make the 3H12 epitope inaccessible. 3H12 recognizes all isoforms.
DSHB Growth Medium :
RPMI
References (legacy) :
J. Cell Biol. 123(3), 523-534.
more info or order :
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Developmental Studies Hybridoma Bank
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