Spitz, extracellular domain | Developmental Studies Hybridoma Bank anti-Spitz product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Spitz, extracellular domain

catalog :

anti-Spitz

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

anti-Spitz

reactivity :

fruit fly

application :

western blot, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Reference
Cruz J, Bota Rabassedas N, Franch Marro X. FGF coordinates air sac development by activation of the EGF ligand Vein through the transcription factor PntP2. Sci Rep. 2015;5:17806 pubmed publisher
Lee Y, Sun Y. Maintenance of glia in the optic lamina is mediated by EGFR signaling by photoreceptors in adult Drosophila. PLoS Genet. 2015;11:e1005187 pubmed publisher
Querenet M, Goubard V, Chatelain G, Davoust N, Mollereau B. Spen is required for pigment cell survival during pupal development in Drosophila. Dev Biol. 2015;402:208-15 pubmed publisher
Steinhauer J, Liu H, Miller E, Treisman J. Trafficking of the EGFR ligand Spitz regulates its signaling activity in polarized tissues. J Cell Sci. 2013;126:4469-78 pubmed publisher
Miura G, Buglino J, Alvarado D, Lemmon M, Resh M, Treisman J. Palmitoylation of the EGFR ligand Spitz by Rasp increases Spitz activity by restricting its diffusion. Dev Cell. 2006;10:167-76 pubmed
Rodrigues A, Werner E, Moses K. Genetic and biochemical analysis of the role of Egfr in the morphogenetic furrow of the developing Drosophila eye. Development. 2005;132:4697-707 pubmed
Tsruya R, Schlesinger A, Reich A, Gabay L, Sapir A, Shilo B. Intracellular trafficking by Star regulates cleavage of the Drosophila EGF receptor ligand Spitz. Genes Dev. 2002;16:222-34 pubmed
Schweitzer R, Shaharabany M, Seger R, Shilo B. Secreted Spitz triggers the DER signaling pathway and is a limiting component in embryonic ventral ectoderm determination. Genes Dev. 1995;9:1518-29 pubmed

product information

Internal ID :

2210

Name :

anti-Spitz

Depositor Name :

Shilo, B.-Z.

Depositor Institution :

Weizmann Institute of Science

Date Deposited :

7/13/05

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Spitz, extracellular domain

Antigen Species :

Drosophila

Host Species :

rat

Isotype :

RIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

7601354

Collections :

Cell signaling,Drosophila antigens

Search Keywords :

Shilo, B.-Z., Spitz, extracellular domain, Drosophila, RIgG2a, Drosophila, spi, CG10334, spitz, AB_528474, monoclonal, Cell signaling/Drosophila antigens, Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: 26 kDa

Gene :

spi

Alternate Gene Name(s) :

CG10334, spitz

Uniprot ID :

Q01083

Antibody Registry ID :

AB_528474

Synonyms (Alternate Clone Names) :

mAb Rtsspi, anti-spi

Immunogen :

Recombinant Drosophila spitz (N-terminal to dibasic signal located between the EGF and transmembrane domains)/GST fusion protein.

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

NSO

Epitope Mapped :

Yes

Epitope Location or Sequence :

N-terminal region, to dibasic signal located between the EGF and transmembrane domains.

Epitope Map PubMed ID :

7601354

Recommended Applications :

Immunofluorescence,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

25872184 11799065 16459296 16207755 26632449

Western Blot Pubmed IDs :

7601354 11799065 16459296 23902690

DSHB Growth Medium :

Iscove's

References (legacy) :

Genes Dev. 9, 1518-1529.

more info or order :

Developmental Studies Hybridoma Bank product webpage