Groucho protein | Developmental Studies Hybridoma Bank anti-Gro product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Groucho protein

catalog :

anti-Gro

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

anti-Gro

reactivity :

fruit fly

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Reference
Webber J, Zhang J, Massey A, Sanchez Luege N, Rebay I. Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness in Drosophila. Development. 2018;145: pubmed publisher
Kwong P, Chambers M, Vashisht A, TURKI JUDEH W, Yau T, Wohlschlegel J, et al. The Central Region of the Drosophila Co-repressor Groucho as a Regulatory Hub. J Biol Chem. 2015;290:30119-30 pubmed publisher
Kiparaki M, Zarifi I, Delidakis C. bHLH proteins involved in Drosophila neurogenesis are mutually regulated at the level of stability. Nucleic Acids Res. 2015;43:2543-59 pubmed publisher
Upadhyai P, Campbell G. Brinker possesses multiple mechanisms for repression because its primary co-repressor, Groucho, may be unavailable in some cell types. Development. 2013;140:4256-65 pubmed publisher
Zarifi I, Kiparaki M, Koumbanakis K, Giagtzoglou N, Zacharioudaki E, Alexiadis A, et al. Essential roles of Da transactivation domains in neurogenesis and in E(spl)-mediated repression. Mol Cell Biol. 2012;32:4534-48 pubmed publisher
Formaz Preston A, Ryu J, Svendsen P, Brook W. The Tbx20 homolog Midline represses wingless in conjunction with Groucho during the maintenance of segment polarity. Dev Biol. 2012;369:319-29 pubmed publisher
TURKI JUDEH W, Courey A. The unconserved groucho central region is essential for viability and modulates target gene specificity. PLoS ONE. 2012;7:e30610 pubmed publisher
Nagel A, Krejci A, Tenin G, Bravo Patiño A, Bray S, Maier D, et al. Hairless-mediated repression of notch target genes requires the combined activity of Groucho and CtBP corepressors. Mol Cell Biol. 2005;25:10433-41 pubmed
Nuthall H, Husain J, McLarren K, Stifani S. Role for Hes1-induced phosphorylation in Groucho-mediated transcriptional repression. Mol Cell Biol. 2002;22:389-99 pubmed
Apidianakis Y, Grbavec D, Stifani S, Delidakis C. Groucho mediates a Ci-independent mechanism of hedgehog repression in the anterior wing pouch. Development. 2001;128:4361-70 pubmed
Delidakis C, Preiss A, Hartley D, Artavanis Tsakonas S. Two genetically and molecularly distinct functions involved in early neurogenesis reside within the Enhancer of split locus of Drosophila melanogaster. Genetics. 1991;129:803-23 pubmed

product information

Internal ID :

2126

Name :

anti-Gro

Depositor Name :

Delidakis, C.

Depositor Institution :

Institute of Molecular Biology & Biotechnology, FORTH, GREECE

Date Deposited :

11/4/03

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Groucho protein

Antigen Species :

Drosophila melanogaster

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

1752423

Collections :

Drosophila antigens,Transcription factors

Search Keywords :

Delidakis, C., Groucho protein, Drosophila melanogaster, MIgG1, kappa light chain, Drosophila, gro, CG8384, Groucho, m9/m10, E(spl)gro, E(spl)m9/10, AB_528272, monoclonal, Drosophila antigens/Transcription factors, Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 80 kDa; Apparent: 80 kDa

Gene :

gro

Alternate Gene Name(s) :

CG8384, Groucho, m9/m10, E(spl)gro, E(spl)m9/10

Uniprot ID :

P16371

Antibody Registry ID :

AB_528272

Immunogen :

Recombinant Gro (aa 120-380, approximately)/GST fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 120-380, approximately

Epitope Map PubMed ID :

1752423

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Immunofluorescence Pubmed IDs :

1752423 11684670 24086079

Western Blot Pubmed IDs :

1752423 26483546 22814213 22319573 25694512 22949507 11756536 16287856

Immunoprecipitation Pubmed IDs :

22814213 11756536

DSHB Growth Medium :

Iscove's

References (legacy) :

Genetics 129, 803-823.; Development 128, 4361-4370.

more info or order :

Developmental Studies Hybridoma Bank product webpage