Bicaudal-D | Developmental Studies Hybridoma Bank anti-Bicaudal-D 4C2 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Bicaudal-D

catalog :

anti-Bicaudal-D 4C2

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

anti-Bicaudal-D 4C2

reactivity :

fruit fly

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 22

Reference
Vazquez Pianzola P, Adam J, Haldemann D, Hain D, Urlaub H, Suter B. Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D. Development. 2014;141:1915-26 pubmed publisher
Vazquez Pianzola P, Urlaub H, Suter B. Pabp binds to the osk 3'UTR and specifically contributes to osk mRNA stability and oocyte accumulation. Dev Biol. 2011;357:404-18 pubmed publisher
Ozdowski E, Mowery Y, Cronmiller C. Stall encodes an ADAMTS metalloprotease and interacts genetically with Delta in Drosophila ovarian follicle formation. Genetics. 2009;183:1027-40 pubmed publisher
Dienstbier M, Boehl F, Li X, Bullock S. Egalitarian is a selective RNA-binding protein linking mRNA localization signals to the dynein motor. Genes Dev. 2009;23:1546-58 pubmed publisher
Koch R, Ledermann R, Urwyler O, Heller M, Suter B. Systematic functional analysis of Bicaudal-D serine phosphorylation and intragenic suppression of a female sterile allele of BicD. PLoS ONE. 2009;4:e4552 pubmed publisher
Larsen K, Xu J, Cermelli S, Shu Z, Gross S. BicaudalD actively regulates microtubule motor activity in lipid droplet transport. PLoS ONE. 2008;3:e3763 pubmed publisher
Boylan K, Mische S, Li M, Marques G, Morin X, Chia W, et al. Motility screen identifies Drosophila IGF-II mRNA-binding protein--zipcode-binding protein acting in oogenesis and synaptogenesis. PLoS Genet. 2008;4:e36 pubmed publisher
Delanoue R, Herpers B, Soetaert J, Davis I, Rabouille C. Drosophila Squid/hnRNP helps Dynein switch from a gurken mRNA transport motor to an ultrastructural static anchor in sponge bodies. Dev Cell. 2007;13:523-38 pubmed
Lecuyer E, Yoshida H, Parthasarathy N, Alm C, Babak T, Cerovina T, et al. Global analysis of mRNA localization reveals a prominent role in organizing cellular architecture and function. Cell. 2007;131:174-87 pubmed
Januschke J, Nicolas E, Compagnon J, Formstecher E, Goud B, Guichet A. Rab6 and the secretory pathway affect oocyte polarity in Drosophila. Development. 2007;134:3419-25 pubmed
Coutelis J, Ephrussi A. Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis. Development. 2007;134:1419-30 pubmed
Mirouse V, Formstecher E, Couderc J. Interaction between Polo and BicD proteins links oocyte determination and meiosis control in Drosophila. Development. 2006;133:4005-13 pubmed
Jenny A, Hachet O, Závorszky P, Cyrklaff A, Weston M, Johnston D, et al. A translation-independent role of oskar RNA in early Drosophila oogenesis. Development. 2006;133:2827-33 pubmed
Delanoue R, Davis I. Dynein anchors its mRNA cargo after apical transport in the Drosophila blastoderm embryo. Cell. 2005;122:97-106 pubmed
Papoulas O, Hays T, Sisson J. The golgin Lava lamp mediates dynein-based Golgi movements during Drosophila cellularization. Nat Cell Biol. 2005;7:612-8 pubmed
Bullock S, Ish Horowicz D. Conserved signals and machinery for RNA transport in Drosophila oogenesis and embryogenesis. Nature. 2001;414:611-6 pubmed
Vied C, Horabin J. The sex determination master switch, Sex-lethal, responds to Hedgehog signaling in the Drosophila germline. Development. 2001;128:2649-60 pubmed
Munn K, Steward R. The shut-down gene of Drosophila melanogaster encodes a novel FK506-binding protein essential for the formation of germline cysts during oogenesis. Genetics. 2000;156:245-56 pubmed
Oh J, Baksa K, Steward R. Functional domains of the Drosophila bicaudal-D protein. Genetics. 2000;154:713-24 pubmed
Bopp D, Horabin J, Lersch R, Cline T, Schedl P. Expression of the Sex-lethal gene is controlled at multiple levels during Drosophila oogenesis. Development. 1993;118:797-812 pubmed
Lantz V, Chang J, Horabin J, Bopp D, Schedl P. The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity. Genes Dev. 1994;8:598-613 pubmed
Suter B, Steward R. Requirement for phosphorylation and localization of the Bicaudal-D protein in Drosophila oocyte differentiation. Cell. 1991;67:917-26 pubmed

product information

Internal ID :

2350

Name :

anti-Bicaudal-D 4C2

Depositor Name :

Steward, R.

Depositor Institution :

Waksman Institute, Rutgers University

Date Deposited :

3/20/03

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Bicaudal-D

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

1959135

Depositor Notes (Special Instructions) :

Fail to detect BicD in some loss-of-function bicD alleles.

Collections :

Cytoskeleton,Drosophila antigens,Neurodevelopment

Search Keywords :

Steward, Ruth, Bicaudal-D, Drosophila, MIgG1, kappa light chain, Drosophila, BicD, almond; anon-EST; Bic-D; Dmel CG6605, AB_528103, monoclonal

Antigen Molecular Weight :

Predicted: 89kDa; Apparent: 97kDa

Gene :

BicD

Alternate Gene Name(s) :

almond; anon-EST; Bic-D; Dmel CG6605

Uniprot ID :

P16568

Antibody Registry ID :

AB_528103

Synonyms (Alternate Clone Names) :

4C2

Immunogen :

Recombinant protein

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

N-terminal (aa 1-543)

Epitope Map PubMed ID :

19515976

Recommended Applications :

Function Blocking,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

19234596 24718986 16971474 19018277 21782810 7523244 10978289 17923096 16835436

Western Blot Pubmed IDs :

1959135 19234596 10655224 17329360 24718986 16971474 19018277 21782810 17827179 15908943 11526072 8076518

Immunoprecipitation Pubmed IDs :

1959135 19234596 10655224 24718986 21782810 11526072

Function Blocking Pubmed IDs :

11740552 16009136

Pubmed IDs :

19752215 18282112 17925228

Depositor Growth Medium :

RPMI

Additional Information :

4C2 and 1B11 (anti-bicaudal-D antibodies) bind to different epitopes on Bicaudal-D; 4C2 binds to a N-terminal epitope (aa 1-543) and 1B11 binds to a C-terminal epitope (aa 544-782). 4C2 and 1B11 have sometimes been combined to increase efficiency of immunoprecipitation.

DSHB Growth Medium :

RPMI

References (legacy) :

Cell 67, 917-926.

more info or order :

Developmental Studies Hybridoma Bank product webpage