Bicaudal-D | Developmental Studies Hybridoma Bank anti-Bicaudal-D 1B11 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Bicaudal-D

catalog :

anti-Bicaudal-D 1B11

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

anti-Bicaudal-D 1B11

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Reference
Vazquez Pianzola P, Schaller B, Colombo M, Beuchle D, Neuenschwander S, Marcil A, et al. The mRNA transportome of the BicD/Egl transport machinery. RNA Biol. 2017;14:73-89 pubmed publisher
Vazquez Pianzola P, Adam J, Haldemann D, Hain D, Urlaub H, Suter B. Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D. Development. 2014;141:1915-26 pubmed publisher
Dix C, Soundararajan H, Dzhindzhev N, Begum F, Suter B, Ohkura H, et al. Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs. J Cell Biol. 2013;202:479-94 pubmed publisher
Bianco A, Dienstbier M, Salter H, Gatto G, Bullock S. Bicaudal-D regulates fragile X mental retardation protein levels, motility, and function during neuronal morphogenesis. Curr Biol. 2010;20:1487-92 pubmed publisher
Dienstbier M, Boehl F, Li X, Bullock S. Egalitarian is a selective RNA-binding protein linking mRNA localization signals to the dynein motor. Genes Dev. 2009;23:1546-58 pubmed publisher
Coutelis J, Ephrussi A. Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis. Development. 2007;134:1419-30 pubmed
Houalla T, Hien Vuong D, Ruan W, Suter B, Rao Y. The Ste20-like kinase misshapen functions together with Bicaudal-D and dynein in driving nuclear migration in the developing drosophila eye. Mech Dev. 2005;122:97-108 pubmed
Navarro C, Puthalakath H, Adams J, Strasser A, Lehmann R. Egalitarian binds dynein light chain to establish oocyte polarity and maintain oocyte fate. Nat Cell Biol. 2004;6:427-35 pubmed
Bullock S, Ish Horowicz D. Conserved signals and machinery for RNA transport in Drosophila oogenesis and embryogenesis. Nature. 2001;414:611-6 pubmed
Oh J, Baksa K, Steward R. Functional domains of the Drosophila bicaudal-D protein. Genetics. 2000;154:713-24 pubmed
Suter B, Steward R. Requirement for phosphorylation and localization of the Bicaudal-D protein in Drosophila oocyte differentiation. Cell. 1991;67:917-26 pubmed

product information

Internal ID :

2344

Name :

anti-Bicaudal-D 1B11

Depositor Name :

Steward, R.

Depositor Institution :

Waksman Institute, Rutgers University

Date Deposited :

3/20/03

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Bicaudal-D

Antigen Species :

drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

1959135

Depositor Notes (Special Instructions) :

Fails to detect BicD in some loss-of-function bicD alleles and alleles missing the C-terminal.

Collections :

Cytoskeleton,Drosophila antigens,Neurodevelopment

Search Keywords :

Steward, R., Bicaudal-D, drosophila, MIgG1, kappa light chain, drosophila, BicD, almond; anon-EST; Bic-D; Dmel CG6605, AB_528102, monoclonal, Cytoskeletal elements/Drosophila antigens/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/Function Blocking

Antigen Molecular Weight :

Predicted: 89kDa; Apparent: 97kDa

Gene :

BicD

Alternate Gene Name(s) :

almond; anon-EST; Bic-D; Dmel CG6605

Uniprot ID :

P16568

Antibody Registry ID :

AB_528102

Synonyms (Alternate Clone Names) :

1B11

Immunogen :

Recombinant protein

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-terminus (aa 544-782)

Epitope Map PubMed ID :

19515976

Recommended Applications :

Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

1959135

Immunofluorescence Pubmed IDs :

24718986 27801632 15582780 17329360 11740552

Western Blot Pubmed IDs :

1959135 24718986 27801632 27801632 23918939 19515976 15582780 10655224 20691595

Immunoprecipitation Pubmed IDs :

1959135 24718986 10655224 20691595 15077115

Function Blocking Pubmed IDs :

19515976

Additional Information :

4C2 and 1B11 (anti-bicaudal-D antibodies) bind to different epitopes on Bicaudal-D; 4C2 binds to a N-terminal epitope (aa 1-543) and 1B11 binds to a C-terminal epitope (aa 544-782). 4C2 and 1B11 have sometimes been combined to increase efficiency of immunoprecipitation.

DSHB Growth Medium :

RPMI

References (legacy) :

Cell 67, 917-926.

more info or order :

Developmental Studies Hybridoma Bank product webpage