Abdominal-B homeobox protein | Developmental Studies Hybridoma Bank anti-ABD-B (1A2E9) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Abdominal-B homeobox protein

catalog :

anti-ABD-B (1A2E9)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

anti-ABD-B (1A2E9)

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 45

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:50; loading ...; fig 2d	Zmojdzian M, de Joussineau S, Da Ponte J, Jagla K. Distinct subsets of Eve-positive pericardial cells stabilise cardiac outflow and contribute to Hox gene-triggered heart morphogenesis in Drosophila. Development. 2018;145: pubmed publisher
immunohistochemistry; fruit fly; 1:20; tbl 1	Alvarez Rivero J, Moris Sanz M, Estacio Gómez A, Montoliu Nerin M, Diaz Benjumea F, Herrero P. Variability in the number of abdominal leucokinergic neurons in adult Drosophila melanogaster. J Comp Neurol. 2017;525:639-660 pubmed publisher
immunohistochemistry; fruit fly; 1:10; fig s2c	Loubiere V, Delest A, Thomas A, Bonev B, Schuettengruber B, Sati S, et al. Coordinate redeployment of PRC1 proteins suppresses tumor formation during Drosophila development. Nat Genet. 2016;48:1436-1442 pubmed publisher
immunohistochemistry; fruit fly; 1:50	Moris Sanz M, Estacio GÃ³mez A, SÃ¡nchez Herrero E, DÃaz Benjumea F. The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B. Biol Open. 2015;4:1132-42 pubmed publisher
	Zhu J, Ordway A, Weber L, Buddika K, Kumar J. Polycomb group (PcG) proteins and Pax6 cooperate to inhibit in vivo reprogramming of the developing Drosophila eye. Development. 2018;145: pubmed publisher
	Tripathy R, Kunwar P, Sano H, Renault A. Transcriptional regulation of Drosophila gonad formation. Dev Biol. 2014;392:193-208 pubmed publisher
	Rogulja Ortmann A, Picao Osorio J, Villava C, Patraquim P, Lafuente E, Aspden J, et al. The RNA-binding protein ELAV regulates Hox RNA processing, expression and function within the Drosophila nervous system. Development. 2014;141:2046-56 pubmed publisher
	Harumoto T, Anbutsu H, Fukatsu T. Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway. PLoS Pathog. 2014;10:e1003956 pubmed publisher
	Bánréti A, Hudry B, Sass M, Saurin A, Graba Y. Hox proteins mediate developmental and environmental control of autophagy. Dev Cell. 2014;28:56-69 pubmed publisher
	Castellanos M, Tang J, Allan D. Female-biased dimorphism underlies a female-specific role for post-embryonic Ilp7 neurons in Drosophila fertility. Development. 2013;140:3915-26 pubmed publisher
	Estacio Gómez A, Moris Sanz M, Schäfer A, Perea D, Herrero P, Diaz Benjumea F. Bithorax-complex genes sculpt the pattern of leucokinergic neurons in the Drosophila central nervous system. Development. 2013;140:2139-48 pubmed publisher
	Baek M, Enriquez J, Mann R. Dual role for Hox genes and Hox co-factors in conferring leg motoneuron survival and identity in Drosophila. Development. 2013;140:2027-38 pubmed publisher
	Rivas M, Espinosa Vázquez J, Sambrani N, Greig S, Merabet S, Graba Y, et al. Antagonism versus cooperativity with TALE cofactors at the base of the functional diversification of Hox protein function. PLoS Genet. 2013;9:e1003252 pubmed publisher
	Wang W, Tindell N, Yan S, Yoder J. Homeotic functions of the Teashirt transcription factor during adult Drosophila development. Biol Open. 2013;2:18-29 pubmed publisher
	Morillo Prado J, Chen X, Fuller M. Polycomb group genes Psc and Su(z)2 maintain somatic stem cell identity and activity in Drosophila. PLoS ONE. 2012;7:e52892 pubmed publisher
	Renault A. vasa is expressed in somatic cells of the embryonic gonad in a sex-specific manner in Drosophila melanogaster. Biol Open. 2012;1:1043-8 pubmed publisher
	Hödl M, Basler K. Transcription in the absence of histone H3.2 and H3K4 methylation. Curr Biol. 2012;22:2253-7 pubmed publisher
	Foronda D, Martin P, Sánchez Herrero E. Drosophila Hox and sex-determination genes control segment elimination through EGFR and extramacrochetae activity. PLoS Genet. 2012;8:e1002874 pubmed publisher
	Wang W, Yoder J. Hox-mediated regulation of doublesex sculpts sex-specific abdomen morphology in Drosophila. Dev Dyn. 2012;241:1076-90 pubmed publisher
	Minami R, Wakabayashi M, Sugimori S, Taniguchi K, Kokuryo A, Imano T, et al. The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila. PLoS ONE. 2012;7:e32302 pubmed publisher
	Lemons D, Pare A, McGinnis W. Three Drosophila Hox complex microRNAs do not have major effects on expression of evolutionarily conserved Hox gene targets during embryogenesis. PLoS ONE. 2012;7:e31365 pubmed publisher
	Stepanik V, Harte P. A mutation in the E(Z) methyltransferase that increases trimethylation of histone H3 lysine 27 and causes inappropriate silencing of active Polycomb target genes. Dev Biol. 2012;364:249-58 pubmed publisher
	Wang W, Kidd B, Carroll S, Yoder J. Sexually dimorphic regulation of the Wingless morphogen controls sex-specific segment number in Drosophila. Proc Natl Acad Sci U S A. 2011;108:11139-44 pubmed publisher
	Gandille P, Narbonne Reveau K, Boissonneau E, Randsholt N, Busson D, Pret A. Mutations in the polycomb group gene polyhomeotic lead to epithelial instability in both the ovary and wing imaginal disc in Drosophila. PLoS ONE. 2010;5:e13946 pubmed publisher
	Bhatia S, Pawar H, Dasari V, Mishra R, Chandrashekaran S, Brahmachari V. Chromatin remodeling protein INO80 has a role in regulation of homeotic gene expression in Drosophila. Genes Cells. 2010;15:725-35 pubmed publisher
	Li X, Han Y, Xi R. Polycomb group genes Psc and Su(z)2 restrict follicle stem cell self-renewal and extrusion by controlling canonical and noncanonical Wnt signaling. Genes Dev. 2010;24:933-46 pubmed publisher
	González I, Aparicio R, Busturia A. Functional characterization of the dRYBP gene in Drosophila. Genetics. 2008;179:1373-88 pubmed publisher
	Miguel Aliaga I, Thor S, Gould A. Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons. PLoS Biol. 2008;6:e58 pubmed publisher
	Salvaing J, Nagel A, Mouchel Vielh E, Bloyer S, Maier D, Preiss A, et al. The enhancer of trithorax and polycomb corto interacts with cyclin G in Drosophila. PLoS ONE. 2008;3:e1658 pubmed publisher
	DeFalco T, Camara N, Le Bras S, Van Doren M. Nonautonomous sex determination controls sexually dimorphic development of the Drosophila gonad. Dev Cell. 2008;14:275-86 pubmed publisher
	Dos Santos N, Rubin T, Chalvet F, Gandille P, Cremazy F, Leroy J, et al. Drosophila retinal pigment cell death is regulated in a position-dependent manner by a cell memory gene. Int J Dev Biol. 2008;52:21-31 pubmed
	Mohan M, Bartkuhn M, Herold M, Philippen A, Heinl N, Bardenhagen I, et al. The Drosophila insulator proteins CTCF and CP190 link enhancer blocking to body patterning. EMBO J. 2007;26:4203-14 pubmed
	Parrish J, Emoto K, Jan L, Jan Y. Polycomb genes interact with the tumor suppressor genes hippo and warts in the maintenance of Drosophila sensory neuron dendrites. Genes Dev. 2007;21:956-72 pubmed
	Hueber S, Bezdan D, Henz S, Blank M, Wu H, Lohmann I. Comparative analysis of Hox downstream genes in Drosophila. Development. 2007;134:381-92 pubmed
	Wang J, Lee C, Lin S, Lee T. Steroid hormone-dependent transformation of polyhomeotic mutant neurons in the Drosophila brain. Development. 2006;133:1231-40 pubmed
	Werz C, Lee T, Lee P, Lackey M, Bolduc C, Stein D, et al. Mis-specified cells die by an active gene-directed process, and inhibition of this death results in cell fate transformation in Drosophila. Development. 2005;132:5343-52 pubmed
	Miguel Aliaga I, Thor S. Segment-specific prevention of pioneer neuron apoptosis by cell-autonomous, postmitotic Hox gene activity. Development. 2004;131:6093-105 pubmed
	DeFalco T, Le Bras S, Van Doren M. Abdominal-B is essential for proper sexually dimorphic development of the Drosophila gonad. Mech Dev. 2004;121:1323-33 pubmed
	Sprecher S, Muller M, Kammermeier L, Miller D, Kaufman T, Reichert H, et al. Hox gene cross-regulatory interactions in the embryonic brain of Drosophila. Mech Dev. 2004;121:527-36 pubmed
	Ponzielli R, Astier M, Chartier A, Gallet A, Therond P, Semeriva M. Heart tube patterning in Drosophila requires integration of axial and segmental information provided by the Bithorax Complex genes and hedgehog signaling. Development. 2002;129:4509-21 pubmed
	Estrada B, Sánchez Herrero E. The Hox gene Abdominal-B antagonizes appendage development in the genital disc of Drosophila. Development. 2001;128:331-9 pubmed
	Hirth F, Hartmann B, Reichert H. Homeotic gene action in embryonic brain development of Drosophila. Development. 1998;125:1579-89 pubmed
	Celniker S, Lewis E. Molecular basis of transabdominal--a sexually dimorphic mutant of the bithorax complex of Drosophila. Proc Natl Acad Sci U S A. 1993;90:1566-70 pubmed
	Celniker S, Keelan D, Lewis E. The molecular genetics of the bithorax complex of Drosophila: characterization of the products of the Abdominal-B domain. Genes Dev. 1989;3:1424-36 pubmed
	Celniker S, Sharma S, Keelan D, Lewis E. The molecular genetics of the bithorax complex of Drosophila: cis-regulation in the Abdominal-B domain. EMBO J. 1990;9:4277-86 pubmed

product information

Internal ID :

1734

Name :

anti-ABD-B (1A2E9)

Depositor Name :

Celniker, S.

Depositor Institution :

Genome Dynamics, Lawrence Berkeley National Lab

Date Deposited :

8/14/02

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Abdominal-B homeobox protein

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

2575066

Depositor Notes (Special Instructions) :

Immunohistochemistry: spatial distribution in 1) wild-type embryos [Genes Dev. 3, 1424-1436], 2) Abd-B mutant embryos [EMBO J. 9, 4277-4286], and 3) Transabdominal mutant wing discs [PNAS USA 90, 1566-1570].

Collections :

Drosophila antigens,Transcription factors

Search Keywords :

Celniker, Susan, Abdominal-B homeobox protein, Drosophila, MIgG1, Drosophila, Abd-B, bex; BX-C; DmAbdB; Dmel CG11648; Droabdb; FAB; , AB_528061, monoclonal

Antigen Molecular Weight :

Predicted: 56 and 30kDa; Apparent: 54 and 36kDa

Gene :

Abd-B

Alternate Gene Name(s) :

bex; BX-C; DmAbdB; Dmel CG11648; Droabdb; FAB;

Uniprot ID :

P09087

Antibody Registry ID :

AB_528061

Synonyms (Alternate Clone Names) :

anti-ABD-B; anti-ABDB; 1A2E9

Immunogen :

Drosophila Abd-B homeodomain/fusion protein trpE/Abd-B fusion protein semi-purified from whole-cell lysates by acrylamide gel electrophoresis

Immunogen Sequence :

270-376 aa region of isoform M

Clonality :

Monoclonal

Myeloma Strain :

HL-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

a.a. 270-376

Epitope Map PubMed ID :

2575066

Recommended Applications :

Gel Supershift,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

8094560 2575066 18286205 22488883 16280349

Immunofluorescence Pubmed IDs :

2575066 16495309 21690416 23213382 24550732 18267095 18336071 15537690 24389064 24803653 21085656 22488883 22182520 22393361 23336073 17437999 15454263 9521896 11152632 22427829 12223408 23285219 20545766 17805343 17166915 22912593 23408901 20439432 24927896 23633511 29530880

Western Blot Pubmed IDs :

2575066

Gel Supershift Pubmed IDs :

23408901

Pubmed IDs :

18033669 23981656 23142044 15172684 23536569 18562658 2265608

Depositor Growth Medium :

Iscove's

DSHB Growth Medium :

Iscove's

References (legacy) :

Genes Dev. 3, 1424-1436.; EMBO J. 9, 4277-4286.; PNAS Sci. USA 90, 1566-1570.

more info or order :

Developmental Studies Hybridoma Bank product webpage