product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
ATPase, (Na(+) K(+)) alpha-1 subunit
catalog :
a6F
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
a6F
reactivity :
human, mouse, rat, dog, chicken, zebrafish
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
citations: 38
Published Application/Species/Sample/Dilution | Reference |
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Belliard A, Gulati G, Duan Q, Alves R, Brewer S, Madan N, et al. Ischemia/reperfusion-induced alterations of enzymatic and signaling functions of the rat cardiac Na+/K+-ATPase: protection by ouabain preconditioning. Physiol Rep. 2016;4: pubmed
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Obara T, Mangos S, Liu Y, Zhao J, Wiessner S, Kramer Zucker A, et al. Polycystin-2 immunolocalization and function in zebrafish. J Am Soc Nephrol. 2006;17:2706-18 pubmed
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Lasaitiene D, Chen Y, Mildaziene V, Nauciene Z, Sundelin B, Johansson B, et al. Tubular mitochondrial alterations in neonatal rats subjected to RAS inhibition. Am J Physiol Renal Physiol. 2006;290:F1260-9 pubmed
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Hlivko J, Chakraborty S, Hlivko T, Sengupta A, James P. The human Na,K-ATPase alpha 4 isoform is a ouabain-sensitive alpha isoform that is expressed in sperm. Mol Reprod Dev. 2006;73:101-15 pubmed
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Lowery L, Sive H. Initial formation of zebrafish brain ventricles occurs independently of circulation and requires the nagie oko and snakehead/atp1a1a.1 gene products. Development. 2005;132:2057-67 pubmed
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Arteaga M, Gutierrez R, Avila J, Mobasheri A, Diaz Flores L, Martin Vasallo P. Regeneration influences expression of the Na+, K+-atpase subunit isoforms in the rat peripheral nervous system. Neuroscience. 2004;129:691-702 pubmed
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Sharma M, Pampinella F, Nemes C, Benharouga M, So J, Du K, et al. Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes. J Cell Biol. 2004;164:923-33 pubmed
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Mobasheri A, Pestov N, Papanicolaou S, Kajee R, Cozar Castellano I, Avila J, et al. Expression and cellular localization of Na,K-ATPase isoforms in the rat ventral prostate. BJU Int. 2003;92:793-802 pubmed
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Suarez E, Bach D, Cadefau J, Palacin M, Zorzano A, Guma A. A novel role of neuregulin in skeletal muscle. Neuregulin stimulates glucose uptake, glucose transporter translocation, and transporter expression in muscle cells. J Biol Chem. 2001;276:18257-64 pubmed
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Hirling H, Steiner P, Chaperon C, Marsault R, Regazzi R, Catsicas S. Syntaxin 13 is a developmentally regulated SNARE involved in neurite outgrowth and endosomal trafficking. Eur J Neurosci. 2000;12:1913-23 pubmed
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Drummond I, Majumdar A, Hentschel H, Elger M, Solnica Krezel L, Schier A, et al. Early development of the zebrafish pronephros and analysis of mutations affecting pronephric function. Development. 1998;125:4655-67 pubmed
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Arystarkhova E, Sweadner K. Isoform-specific monoclonal antibodies to Na,K-ATPase alpha subunits. Evidence for a tissue-specific post-translational modification of the alpha subunit. J Biol Chem. 1996;271:23407-17 pubmed
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Takeyasu K, Tamkun M, Renaud K, Fambrough D. Ouabain-sensitive (Na+ + K+)-ATPase activity expressed in mouse L cells by transfection with DNA encoding the alpha-subunit of an avian sodium pump. J Biol Chem. 1988;263:4347-54 pubmed
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product information
Internal ID :
2635
Name :
a6F
Depositor Name :
Fambrough, D.M.
Depositor Institution :
Johns Hopkins School of Medicine
Depositor Website :
http://www.searlescholars.net/person/470
Date Deposited :
2/2/96
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
ATPase, (Na(+) K(+)) alpha-1 subunit
Antigen Species :
Chicken
Host Species :
mouse
Isotype :
MIgG2a, kappa light chain
Isotype for catalog (legacy) :
IgG2a, kappa light chain
Positive Tested Species Reactivity :
Avian,Canine,Fish,Human,Mouse,Porcine,Rat,Torpedo,Zebrafish
Species Tested (legacy) :
avian; human, dog, mouse, rat, pig, torpedo, zebrafish. Weak or no binding to sheep and Xenopus
Initial Publication Pubmed ID :
2831227
Depositor Notes (Special Instructions) :
Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear, and located on the N-terminal region between aa 27-55. Weak or no binding to sheep and Xenopus. Works great for Immunoblotting. Immunochemistry: good on cryosections; poor for lightly-fixed cells in tissue culture; seems to require denaturation of Na, K-ATPase. This hybridoma was made together with alpha-7C, described in [PMID 2831227].
Collections :
Cardiac development,Enzymes,Human,Neurodevelopment,Skeletal muscle
Search Keywords :
Fambrough, D. M., ATPase, (Na(+) K(+)) alpha-1 subunit, Chicken, MIgG2a, kappa light chain, Avian/Human/Canine/Mouse/Rat/Porcine/Torpedo/Zebrafish/Fish, ATP1A1, , AB_528092, monoclonal, Cardiac development/Enzymes/Human/Neurodevelopment/Skeletal muscle, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/ELISA
Antigen Molecular Weight :
112 kDa
Gene :
ATP1A1
Uniprot ID :
P09572
Antibody Registry ID :
AB_528092
Synonyms (Alternate Clone Names) :
6F; alpha6F; Atp1a1
Immunogen :
purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.
Immunogen Sequence :
Total protein
Clonality :
Monoclonal
Myeloma Strain :
SP 2/0 Ag8
Epitope Mapped :
Yes
Epitope Location or Sequence :
N-terminal region between aa 27-55 [Depositor Notes]. In the 160 aa near the N-terminus, probably between aa 34-52 [PMID 8798546].
Epitope Map PubMed ID :
8798546
Recommended Applications :
ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Great
Immunohistochemistry Pubmed IDs :
9806915 11193188 16249276 21925491 20065300 25914646
Immunofluorescence Pubmed IDs :
9806915 12647783 15541890 22451674 23195960 23288841 23192345 15788456 22848617 16943304 29694368 31425744
Western Blot Pubmed IDs :
27702882 26861875 11278386 12647783 8798546 16249276 22451674 16175638 23192345 19484743 20126465 30086768 31318482 31425744
Immunoprecipitation Pubmed IDs :
23195960 20841356
ELISA Pubmed IDs :
8798546
Pubmed IDs :
14616469 10886332
Additional Information :
Broad species specificity [PMID 8798546]. This antibody doesn't appear to work on conventionally fixed tissue, and stains fixed sections of the rat kidney faintly, but this staining is considerably increased by prior treatment of the sections with SDS [PMID 8798546]. RRID: AB_528092
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Biol. Chem. 263, 4347-4354.; Development 125, 4655-4667.; J. Biol. Chem. 276(21), 18257-18264.; Histol. Histopathol. 16, 141-154.; Histol. Histopathol. 18, 343-351.; BJU International 92, 793-802.; Neuroscience 129, 691-702.; Am. J. Physiol. Renal Physiol. 290, F1260-F1269.; Dev. Biol. 359, 95-107.; J. Biol. Chem. 287(20), 16424-16434.; . J. Biol. Chem. 288(4), 2734-2743.; J. Biol. Chem. 288(8), 5803-5814.
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