ATPase, (Na(+) K(+)) alpha-1 subunit | Developmental Studies Hybridoma Bank a6F product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

ATPase, (Na(+) K(+)) alpha-1 subunit

catalog :

a6F

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

a6F

reactivity :

human, mouse, rat, dog, chicken, zebrafish

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 38

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:750; loading ...; fig 2d	Wyckelsma V, Levinger I, Murphy R, Petersen A, Perry B, Hedges C, et al. Intense interval training in healthy older adults increases skeletal muscle [3H]ouabain-binding site content and elevates Na+,K+-ATPase ?2 isoform abundance in Type II fibers. Physiol Rep. 2017;5: pubmed publisher
western blot; human; 1:750; loading ...; fig 5a	Perry B, Wyckelsma V, Murphy R, Steward C, Anderson M, Levinger I, et al. Dissociation between short-term unloading and resistance training effects on skeletal muscle Na+,K+-ATPase, muscle function, and fatigue in humans. J Appl Physiol (1985). 2016;121:1074-1086 pubmed publisher
immunohistochemistry; zebrafish ; 1:200; loading ...; fig 9a	Prabhudesai S, Bensabeur F, Abdullah R, Basak I, Baez S, Alves G, et al. LRRK2 knockdown in zebrafish causes developmental defects, neuronal loss, and synuclein aggregation. J Neurosci Res. 2016;94:717-35 pubmed publisher
western blot; human; 1:2000; fig 1	Prior K, Wittig I, Leisegang M, Groenendyk J, Weissmann N, Michalak M, et al. The Endoplasmic Reticulum Chaperone Calnexin Is a NADPH Oxidase NOX4 Interacting Protein. J Biol Chem. 2016;291:7045-59 pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig s1a	Hilbers F, Kopeć W, Isaksen T, Holm T, Lykke Hartmann K, Nissen P, et al. Tuning of the Na,K-ATPase by the beta subunit. Sci Rep. 2016;6:20442 pubmed publisher
western blot; human; 1:750; fig 1	Wyckelsma V, McKenna M, Levinger I, Petersen A, Lamboley C, Murphy R. Cell specific differences in the protein abundances of GAPDH and Na(+),K(+)-ATPase in skeletal muscle from aged individuals. Exp Gerontol. 2016;75:8-15 pubmed publisher
western blot; human; fig 1 western blot; mouse; 1:500; fig 1	Sharma P, Abbasi C, Lazic S, Teng A, Wang D, Dubois N, et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun. 2015;6:8391 pubmed publisher
western blot; mouse; 1:1000; fig 5	Kang J, Shen W, Zhou C, Xu D, Macdonald R. The human epilepsy mutation GABRG2(Q390X) causes chronic subunit accumulation and neurodegeneration. Nat Neurosci. 2015;18:988-96 pubmed publisher
western blot; mouse; 1:100	Zhou C, Ding L, Deel M, Ferrick E, Emeson R, Gallagher M. Altered intrathalamic GABAA neurotransmission in a mouse model of a human genetic absence epilepsy syndrome. Neurobiol Dis. 2015;73:407-17 pubmed publisher
immunohistochemistry; rat	Schuth O, McLean W, Eatock R, Pyott S. Distribution of Na,K-ATPase α subunits in rat vestibular sensory epithelia. J Assoc Res Otolaryngol. 2014;15:739-54 pubmed publisher
western blot; mouse; 1:100	Zhou C, Huang Z, Ding L, Deel M, Arain F, Murray C, et al. Altered cortical GABAA receptor composition, physiology, and endocytosis in a mouse model of a human genetic absence epilepsy syndrome. J Biol Chem. 2013;288:21458-72 pubmed publisher
	Sanna Cherchi S, Khan K, Westland R, Krithivasan P, Fievet L, Rasouly H, et al. Exome-wide Association Study Identifies GREB1L Mutations in Congenital Kidney Malformations. Am J Hum Genet. 2017;101:789-802 pubmed publisher
	Belliard A, Gulati G, Duan Q, Alves R, Brewer S, Madan N, et al. Ischemia/reperfusion-induced alterations of enzymatic and signaling functions of the rat cardiac Na+/K+-ATPase: protection by ouabain preconditioning. Physiol Rep. 2016;4: pubmed
	Nardi F, Hoffmann T, Stretton C, Cwiklinski E, Taylor P, Hundal H. Proteasomal modulation of cellular SNAT2 (SLC38A2) abundance and function by unsaturated fatty acid availability. J Biol Chem. 2015;290:8173-84 pubmed publisher
	Ye Q, Lai F, Banerjee M, Duan Q, Li Z, Si S, et al. Expression of mutant ?1 Na/K-ATPase defective in conformational transition attenuates Src-mediated signal transduction. J Biol Chem. 2013;288:5803-14 pubmed publisher
	Azarias G, Kruusmägi M, Connor S, Akkuratov E, Liu X, Lyons D, et al. A specific and essential role for Na,K-ATPase α3 in neurons co-expressing α1 and α3. J Biol Chem. 2013;288:2734-43 pubmed publisher
	Radzyukevich T, Neumann J, Rindler T, Oshiro N, Goldhamer D, Lingrel J, et al. Tissue-specific role of the Na,K-ATPase ?2 isozyme in skeletal muscle. J Biol Chem. 2013;288:1226-37 pubmed publisher
	Mitra S, Lukianov S, Ruiz W, Cianciolo Cosentino C, Sanker S, Traub L, et al. Requirement for a uroplakin 3a-like protein in the development of zebrafish pronephric tubule epithelial cell function, morphogenesis, and polarity. PLoS ONE. 2012;7:e41816 pubmed publisher
	Li X, McClellan M, Tanito M, Garteiser P, Towner R, Bissig D, et al. Loss of caveolin-1 impairs retinal function due to disturbance of subretinal microenvironment. J Biol Chem. 2012;287:16424-34 pubmed publisher
	Trowe M, Maier H, Petry M, Schweizer M, Schuster Gossler K, Kispert A. Impaired stria vascularis integrity upon loss of E-cadherin in basal cells. Dev Biol. 2011;359:95-107 pubmed publisher
	Soltoff S, Asara J, Hedden L. Regulation and identification of Na,K-ATPase alpha1 subunit phosphorylation in rat parotid acinar cells. J Biol Chem. 2010;285:36330-8 pubmed publisher
	Becirovic E, Nakova K, Hammelmann V, Hennel R, Biel M, Michalakis S. The retinitis pigmentosa mutation c.3444+1G>A in CNGB1 results in skipping of exon 32. PLoS ONE. 2010;5:e8969 pubmed publisher
	Floyd R, Wray S, Quenby S, Martín Vasallo P, Mobasheri A. Expression and distribution of Na, K-ATPase isoforms in the human uterus. Reprod Sci. 2010;17:366-76 pubmed publisher
	Tang C, Chiu Y, Tsai S, Lee T. Relative changes in the abundance of branchial Na(+)/K(+)-ATPase alpha-isoform-like proteins in marine euryhaline milkfish (Chanos chanos) acclimated to environments of different salinities. J Exp Zool A Ecol Genet Physiol. 2009;311:521-9 pubmed publisher
	Obara T, Mangos S, Liu Y, Zhao J, Wiessner S, Kramer Zucker A, et al. Polycystin-2 immunolocalization and function in zebrafish. J Am Soc Nephrol. 2006;17:2706-18 pubmed
	Lasaitiene D, Chen Y, Mildaziene V, Nauciene Z, Sundelin B, Johansson B, et al. Tubular mitochondrial alterations in neonatal rats subjected to RAS inhibition. Am J Physiol Renal Physiol. 2006;290:F1260-9 pubmed
	Hlivko J, Chakraborty S, Hlivko T, Sengupta A, James P. The human Na,K-ATPase alpha 4 isoform is a ouabain-sensitive alpha isoform that is expressed in sperm. Mol Reprod Dev. 2006;73:101-15 pubmed
	Lowery L, Sive H. Initial formation of zebrafish brain ventricles occurs independently of circulation and requires the nagie oko and snakehead/atp1a1a.1 gene products. Development. 2005;132:2057-67 pubmed
	Arteaga M, Gutierrez R, Avila J, Mobasheri A, Diaz Flores L, Martin Vasallo P. Regeneration influences expression of the Na+, K+-atpase subunit isoforms in the rat peripheral nervous system. Neuroscience. 2004;129:691-702 pubmed
	Sharma M, Pampinella F, Nemes C, Benharouga M, So J, Du K, et al. Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes. J Cell Biol. 2004;164:923-33 pubmed
	Mobasheri A, Pestov N, Papanicolaou S, Kajee R, Cozar Castellano I, Avila J, et al. Expression and cellular localization of Na,K-ATPase isoforms in the rat ventral prostate. BJU Int. 2003;92:793-802 pubmed
	Shakibaei M, Mobasheri A. Beta1-integrins co-localize with Na, K-ATPase, epithelial sodium channels (ENaC) and voltage activated calcium channels (VACC) in mechanoreceptor complexes of mouse limb-bud chondrocytes. Histol Histopathol. 2003;18:343-51 pubmed publisher
	Suarez E, Bach D, Cadefau J, Palacin M, Zorzano A, Guma A. A novel role of neuregulin in skeletal muscle. Neuregulin stimulates glucose uptake, glucose transporter translocation, and transporter expression in muscle cells. J Biol Chem. 2001;276:18257-64 pubmed
	Mobasheri A, Oukrif D, Dawodu S, Sinha M, Greenwell P, Stewart D, et al. Isoforms of Na+, K+-ATPase in human prostate; specificity of expression and apical membrane polarization. Histol Histopathol. 2001;16:141-54 pubmed publisher
	Hirling H, Steiner P, Chaperon C, Marsault R, Regazzi R, Catsicas S. Syntaxin 13 is a developmentally regulated SNARE involved in neurite outgrowth and endosomal trafficking. Eur J Neurosci. 2000;12:1913-23 pubmed
	Drummond I, Majumdar A, Hentschel H, Elger M, Solnica Krezel L, Schier A, et al. Early development of the zebrafish pronephros and analysis of mutations affecting pronephric function. Development. 1998;125:4655-67 pubmed
	Arystarkhova E, Sweadner K. Isoform-specific monoclonal antibodies to Na,K-ATPase alpha subunits. Evidence for a tissue-specific post-translational modification of the alpha subunit. J Biol Chem. 1996;271:23407-17 pubmed
	Takeyasu K, Tamkun M, Renaud K, Fambrough D. Ouabain-sensitive (Na+ + K+)-ATPase activity expressed in mouse L cells by transfection with DNA encoding the alpha-subunit of an avian sodium pump. J Biol Chem. 1988;263:4347-54 pubmed

product information

Internal ID :

2635

Name :

a6F

Depositor Name :

Fambrough, D.M.

Depositor Institution :

Johns Hopkins School of Medicine

Depositor Website :

http://www.searlescholars.net/person/470

Date Deposited :

2/2/96

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

ATPase, (Na(+) K(+)) alpha-1 subunit

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG2a, kappa light chain

Isotype for catalog (legacy) :

IgG2a, kappa light chain

Positive Tested Species Reactivity :

Avian,Canine,Fish,Human,Mouse,Porcine,Rat,Torpedo,Zebrafish

Species Tested (legacy) :

avian; human, dog, mouse, rat, pig, torpedo, zebrafish. Weak or no binding to sheep and Xenopus

Initial Publication Pubmed ID :

2831227

Depositor Notes (Special Instructions) :

Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear, and located on the N-terminal region between aa 27-55. Weak or no binding to sheep and Xenopus. Works great for Immunoblotting. Immunochemistry: good on cryosections; poor for lightly-fixed cells in tissue culture; seems to require denaturation of Na, K-ATPase. This hybridoma was made together with alpha-7C, described in [PMID 2831227].

Collections :

Cardiac development,Enzymes,Human,Neurodevelopment,Skeletal muscle

Search Keywords :

Fambrough, D. M., ATPase, (Na(+) K(+)) alpha-1 subunit, Chicken, MIgG2a, kappa light chain, Avian/Human/Canine/Mouse/Rat/Porcine/Torpedo/Zebrafish/Fish, ATP1A1, , AB_528092, monoclonal, Cardiac development/Enzymes/Human/Neurodevelopment/Skeletal muscle, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/ELISA

Antigen Molecular Weight :

112 kDa

Gene :

ATP1A1

Uniprot ID :

P09572

Antibody Registry ID :

AB_528092

Synonyms (Alternate Clone Names) :

6F; alpha6F; Atp1a1

Immunogen :

purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

SP 2/0 Ag8

Epitope Mapped :

Yes

Epitope Location or Sequence :

N-terminal region between aa 27-55 [Depositor Notes]. In the 160 aa near the N-terminus, probably between aa 34-52 [PMID 8798546].

Epitope Map PubMed ID :

8798546

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Great

Immunohistochemistry Pubmed IDs :

9806915 11193188 16249276 21925491 20065300 25914646

Immunofluorescence Pubmed IDs :

9806915 12647783 15541890 22451674 23195960 23288841 23192345 15788456 22848617 16943304 29694368 31425744

Western Blot Pubmed IDs :

27702882 26861875 11278386 12647783 8798546 16249276 22451674 16175638 23192345 19484743 20126465 30086768 31318482 31425744

Immunoprecipitation Pubmed IDs :

23195960 20841356

ELISA Pubmed IDs :

8798546

Pubmed IDs :

14616469 10886332

Additional Information :

Broad species specificity [PMID 8798546]. This antibody doesn't appear to work on conventionally fixed tissue, and stains fixed sections of the rat kidney faintly, but this staining is considerably increased by prior treatment of the sections with SDS [PMID 8798546]. RRID: AB_528092

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Biol. Chem. 263, 4347-4354.; Development 125, 4655-4667.; J. Biol. Chem. 276(21), 18257-18264.; Histol. Histopathol. 16, 141-154.; Histol. Histopathol. 18, 343-351.; BJU International 92, 793-802.; Neuroscience 129, 691-702.; Am. J. Physiol. Renal Physiol. 290, F1260-F1269.; Dev. Biol. 359, 95-107.; J. Biol. Chem. 287(20), 16424-16434.; . J. Biol. Chem. 288(4), 2734-2743.; J. Biol. Chem. 288(8), 5803-5814.

more info or order :

Developmental Studies Hybridoma Bank product webpage