product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
ATPase, (Na (+) K(+)) alpha subunit
catalog :
a5
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A5
reactivity :
Mongolian jird, human, mouse, rat, chicken, fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 29
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:100; loading ...; fig 7a
Aguilar J, Cheng M, Font J, Schwartz A, Ledwitch K, Duran A, et al. Psychomotor impairments and therapeutic implications revealed by a mutation associated with infantile Parkinsonism-Dystonia. elife. 2021;10: pubmed publisher
  • immunocytochemistry; human; 1:100
  • western blot; human; loading ...; fig 4a
Chapuis J, Flaig A, Grenier Boley B, Eysert F, Pottiez V, Deloison G, et al. Genome-wide, high-content siRNA screening identifies the Alzheimer's genetic risk factor FERMT2 as a major modulator of APP metabolism. Acta Neuropathol. 2017;133:955-966 pubmed publisher
  • western blot; rat; fig s2
Fan S, Numata Y, Numata M. Endosomal Na+/H+ exchanger NHE5 influences MET recycling and cell migration. Mol Biol Cell. 2016;27:702-15 pubmed publisher
  • western blot; mouse; fig 3
Zulliger R, Conley S, Mwoyosvi M, Stuck M, Azadi S, Naash M. SNAREs Interact with Retinal Degeneration Slow and Rod Outer Segment Membrane Protein-1 during Conventional and Unconventional Outer Segment Targeting. PLoS ONE. 2015;10:e0138508 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:100
Nie J, Mahato S, Zelhof A. Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation. BMC Dev Biol. 2015;15:10 pubmed publisher
  • immunohistochemistry; Mongolian jird; 1:1000
Trattner B, Gravot C, Grothe B, Kunz L. Metabolic Maturation of Auditory Neurones in the Superior Olivary Complex. PLoS ONE. 2013;8:e67351 pubmed publisher
  • immunohistochemistry; mouse
  • western blot; mouse
Gao N, Kaestner K. Cdx2 regulates endo-lysosomal function and epithelial cell polarity. Genes Dev. 2010;24:1295-305 pubmed publisher
Blondeau Bidet E, Hiroi J, Lorin Nebel C. Ion uptake pathways in European sea bass Dicentrarchus labrax. Gene. 2019;692:126-137 pubmed publisher
Horng J, Yu L, Liu S, Chen P, Lin L. Potassium Regulation in Medaka (Oryzias latipes) Larvae Acclimated to Fresh Water: Passive Uptake and Active Secretion by the Skin Cells. Sci Rep. 2017;7:16215 pubmed publisher
Moleirinho S, Hoxha S, Mandati V, Curtale G, Troutman S, Ehmer U, et al. Regulation of localization and function of the transcriptional co-activator YAP by angiomotin. elife. 2017;6: pubmed publisher
Dalla S, Dobler S. Gene duplications circumvent trade-offs in enzyme function: Insect adaptation to toxic host plants. Evolution. 2016;70:2767-2777 pubmed publisher
Richter K, Rolke D, Blenau W, Baumann O. Secretory cells in honeybee hypopharyngeal gland: polarized organization and age-dependent dynamics of plasma membrane. Cell Tissue Res. 2016;366:163-74 pubmed publisher
Chasiotis H, Ionescu A, Misyura L, Bui P, Fazio K, Wang J, et al. An animal homolog of plant Mep/Amt transporters promotes ammonia excretion by the anal papillae of the disease vector mosquito Aedes aegypti. J Exp Biol. 2016;219:1346-55 pubmed publisher
Tang C, Leu M, Yang W, Tsai S. Exploration of the mechanisms of protein quality control and osmoregulation in gills of Chromis viridis in response to reduced salinity. Fish Physiol Biochem. 2014;40:1533-46 pubmed publisher
Jensen L, Farook M, Reiter L. Proteomic profiling in Drosophila reveals potential Dube3a regulation of the actin cytoskeleton and neuronal homeostasis. PLoS ONE. 2013;8:e61952 pubmed publisher
Yang W, Kang C, Chang C, Hsu A, Lee T, Hwang P. Expression profiles of branchial FXYD proteins in the brackish medaka Oryzias dancena: a potential saltwater fish model for studies of osmoregulation. PLoS ONE. 2013;8:e55470 pubmed publisher
Nelson K, Khan Z, Molnar I, Mihaly J, Kaschube M, Beitel G. Drosophila Src regulates anisotropic apical surface growth to control epithelial tube size. Nat Cell Biol. 2012;14:518-25 pubmed publisher
Sakamori R, Das S, Yu S, Feng S, Stypulkowski E, Guan Y, et al. Cdc42 and Rab8a are critical for intestinal stem cell division, survival, and differentiation in mice. J Clin Invest. 2012;122:1052-65 pubmed publisher
Linser P, Neira Oviedo M, Hirata T, Seron T, Smith K, Piermarini P, et al. Slc4-like anion transporters of the larval mosquito alimentary canal. J Insect Physiol. 2012;58:551-62 pubmed publisher
Smith K, Vanekeris L, Linser P. Cloning and characterization of AgCA9, a novel alpha-carbonic anhydrase from Anopheles gambiae Giles sensu stricto (Diptera: Culicidae) larvae. J Exp Biol. 2007;210:3919-30 pubmed publisher
Lee J, Koh H, Kim M, Kim Y, Lee S, Karess R, et al. Energy-dependent regulation of cell structure by AMP-activated protein kinase. Nature. 2007;447:1017-20 pubmed
Patrick M, Aimanova K, Sanders H, Gill S. P-type Na+/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquito Aedes aegypti. J Exp Biol. 2006;209:4638-51 pubmed
Nebel C, Romestand B, Nègre Sadargues G, Grousset E, Aujoulat F, Bacal J, et al. Differential freshwater adaptation in juvenile sea-bass Dicentrarchus labrax: involvement of gills and urinary system. J Exp Biol. 2005;208:3859-71 pubmed
Khodabandeh S, Charmantier G, Charmantier Daures M. Ultrastructural studies and Na+,K+-ATPase immunolocalization in the antennal urinary glands of the lobster Homarus gammarus (Crustacea, Decapoda). J Histochem Cytochem. 2005;53:1203-14 pubmed
Martinez A, Charmantier G, Compère P, Charmantier Daures M. Branchial chamber tissues in two caridean shrimps: the epibenthic Palaemon adspersus and the deep-sea hydrothermal Rimicaris exoculata. Tissue Cell. 2005;37:153-65 pubmed
Lignot J, Susanto G, Charmantier Daures M, Charmantier G. Immunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda). Cell Tissue Res. 2005;319:331-9 pubmed
Khodabandeh S, Kutnik M, Aujoulat F, Charmantier G, Charmantier Daures M. Ontogeny of the antennal glands in the crayfish Astacus leptodactylus (Crustacea, Decapoda): immunolocalization of Na+,K+-ATPase. Cell Tissue Res. 2005;319:167-74 pubmed
Weiner J, Koo S, Nicolas S, Fraboulet S, Pfaff S, Pourquie O, et al. Axon fasciculation defects and retinal dysplasias in mice lacking the immunoglobulin superfamily adhesion molecule BEN/ALCAM/SC1. Mol Cell Neurosci. 2004;27:59-69 pubmed
Lebovitz R, Takeyasu K, Fambrough D. Molecular characterization and expression of the (Na+ + K+)-ATPase alpha-subunit in Drosophila melanogaster. EMBO J. 1989;8:193-202 pubmed
product information
Internal ID :
2623
Name :
a5
Depositor Name :
Fambrough, D.M.
Depositor Institution :
The Johns Hopkins University
Date Deposited :
10/10/90
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
ATPase, (Na (+) K(+)) alpha subunit
Antigen Species :
Chicken
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Avian,Fish,Frog,Honeybee,Human,Insect,Mammal,Mosquito
Species Tested (legacy) :
avian; mammalian; frog; insect; fish
Initial Publication Pubmed ID :
2540956
Depositor Notes (Special Instructions) :
Antibody binds a cytosolic epitope on the alpha-subunit of all Na, K-ATPase isoforms. IP recognizes denatured ATPase only.
Collections :
Cell signaling,Drosophila antigens,Enzymes,Human,Skeletal muscle,Xenopus
Search Keywords :
Fambrough, Douglas M. , ATPase, (Na (+) K(+)) alpha subunit, Chicken, MIgG1, kappa light chain, Avian/Fish/ Frog/Insect/Mammal/Human, ATP1A1, Sodium pump subunit alpha-1, AB_2166869, Monoclonal, drosophila
Antigen Molecular Weight :
112.23 kDa
Gene :
ATP1A1; ATPA2; ATP1A3; ATP1A4
Alternate Gene Name(s) :
Sodium pump subunit alpha-1
Uniprot ID :
P09572
Antibody Registry ID :
AB_2166869
Immunogen :
IP purified from kidney
Immunogen Sequence :
alpha1-beta1 subunits
Clonality :
Monoclonal
Myeloma Strain :
SP2/0 Ag8
Epitope Mapped :
No
Recommended Applications :
FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Great
Immunohistochemistry Pubmed IDs :
23383199 17114398 15345243 22446737
Immunofluorescence Pubmed IDs :
22354172 20551175 17114398 17486097 26944496 27683239 27210106 29176723 30641214
Western Blot Pubmed IDs :
23626758 23383199 24805086 17486097
Immunoprecipitation Pubmed IDs :
23383199
FFPE Pubmed IDs :
26944496
Pubmed IDs :
17981859 22251674
DSHB Growth Medium :
Iscove's
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US