ATPase, (Na(+) K(+)) alpha-1 subunit | Developmental Studies Hybridoma Bank a2F product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

ATPase, (Na(+) K(+)) alpha-1 subunit

catalog :

a2F

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

a2F

reactivity :

human, mouse, dog, chicken

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 6

Reference
Farah C, Nguyen M, Julien J, Leclerc N. Altered levels and distribution of microtubule-associated proteins before disease onset in a mouse model of amyotrophic lateral sclerosis. J Neurochem. 2003;84:77-86 pubmed
Wilson P, Devuyst O, Li X, Gatti L, Falkenstein D, Robinson S, et al. Apical plasma membrane mispolarization of NaK-ATPase in polycystic kidney disease epithelia is associated with aberrant expression of the beta2 isoform. Am J Pathol. 2000;156:253-68 pubmed
Xie Z, Wang Y, Liu G, Zolotarjova N, Periyasamy S, Askari A. Similarities and differences between the properties of native and recombinant Na+/K+-ATPases. Arch Biochem Biophys. 1996;330:153-62 pubmed
Simon S, Holland V, Benos D, Zampighi G. Transcellular and paracellular pathways in lingual epithelia and their influence in taste transduction. Microsc Res Tech. 1993;26:196-208 pubmed
Takeyasu K, Tamkun M, Renaud K, Fambrough D. Ouabain-sensitive (Na+ + K+)-ATPase activity expressed in mouse L cells by transfection with DNA encoding the alpha-subunit of an avian sodium pump. J Biol Chem. 1988;263:4347-54 pubmed
Contreras R, Avila G, Gutierrez C, Bolivar J, Gonzalez Mariscal L, Darzon A, et al. Repolarization of Na+-K+ pumps during establishment of epithelial monolayers. Am J Physiol. 1989;257:C896-905 pubmed

product information

Internal ID :

2611

Name :

a2F

Depositor Name :

Fambrough, D.M.

Depositor Institution :

Johns Hopkins School of Medicine

Depositor Website :

http://www.searlescholars.net/person/470

Date Deposited :

10/10/90

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

ATPase, (Na(+) K(+)) alpha-1 subunit

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG2a, kappa light chain

Isotype for catalog (legacy) :

IgG

Positive Tested Species Reactivity :

Avian,Canine,Human,Mouse,Mustelid,Ovine

Species Tested (legacy) :

avian; dog, mink, sheep. negative on pig.

Initial Publication Pubmed ID :

2831227

Depositor Notes (Special Instructions) :

Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear. Immunohistochemistry: good on cryosections; poor for lightly-fixed cells in tissue culture; seems to require denaturation of Na, K-ATPase. Immunoprecipitation: weak. Negative on Porcine.

Collections :

Cardiac development,Enzymes,Human,Neurodevelopment,Skeletal muscle

Search Keywords :

Fambrough, D. M., ATPase, (Na(+) K(+)) alpha-1 subunit, Chicken, MIgG, Avian/Canine/Mustelid/Ovine/Human/Mouse, ATP1A1, , AB_528090, monoclonal, Cardiac development/Enzymes/Human/Neurodevelopment/Skeletal muscle, Immunohistochemistry/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

112 kDa

Gene :

ATP1A1

Uniprot ID :

P09572

Antibody Registry ID :

AB_528090

Synonyms (Alternate Clone Names) :

2F; alpha2F

Immunogen :

purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit.

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0 Ag8

Epitope Mapped :

Recommended Applications :

Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

10623674 8241559

Western Blot Pubmed IDs :

8651690 12485403

Immunoprecipitation Pubmed IDs :

10623674 2480715

Additional Information :

Reacts poorly with rat.

DSHB Growth Medium :

Iscove's

more info or order :

Developmental Studies Hybridoma Bank product webpage