product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Collagen, type X
catalog :
X-AC9
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
X-AC9
reactivity :
human, mouse, chicken, zebrafish
application :
immunohistochemistry, immunocytochemistry
citations: 2
Reference
Bond S, Lau A, Penuela S, Sampaio A, Underhill T, Laird D, et al. Pannexin 3 is a novel target for Runx2, expressed by osteoblasts and mature growth plate chondrocytes. J Bone Miner Res. 2011;26:2911-22 pubmed publisher
Ohlig S, Farshi P, Pickhinke U, van den Boom J, Höing S, Jakuschev S, et al. Sonic hedgehog shedding results in functional activation of the solubilized protein. Dev Cell. 2011;20:764-74 pubmed publisher
product information
Internal ID :
3020
Name :
X-AC9
Depositor Name :
Linsenmayer, T.F.
Depositor Institution :
Tufts Medical School
Depositor Website :
http://sackler.tufts.edu/Faculty-and-Research/Faculty-Research-Pages/Thomas-Linsenmayer
Date Deposited :
2/11/99
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Collagen, type X
Antigen Species :
Chicken
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Chicken,Cuttlefish,Human,Mouse,Zebrafish
Species Tested (legacy) :
chicken, cuttlefish, mouse
Initial Publication Pubmed ID :
2578471
Depositor Notes (Special Instructions) :
Immunostaining requires pretreatment with 0.5% testicular hyaluronidase (500U/mg) for 1 hr at 37°C.
Collections :
Extracellular matrix proteins,Skeletal muscle
Search Keywords :
Linsenmayer, Thomas F. , Collagen, type X, Chicken, MIgG1, Chicken Mouse Cuttlefish Zebrafish Human, COL10A1, , AB_528175, Monoclonal, Extracellular matrix proteins, Immunohistochemistry Immunofluorescence
Antigen Molecular Weight :
66.4 kDa
Gene :
COL10A1
Uniprot ID :
P08125
Antibody Registry ID :
AB_528175
Immunogen :
Conditioned supernatant from chick chondrocytes in culture
Immunogen Sequence :
Ammonium sulfate precipitated collagen type X
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
Yes
Epitope Location or Sequence :
The epitope lies near the C-terminus of the major collagenase fragment within the triple helical domain of type X collagen.
Epitope Map PubMed ID :
3005323
Recommended Applications :
Immunofluorescence,Immunohistochemistry
Immunohistochemistry Pubmed IDs :
2578471 19432533
Immunofluorescence Pubmed IDs :
2578471 25974685 23909483 22310837 22548797 22595668 21915903 20021685 12697705 3527817
ELISA Pubmed IDs :
2578471
Additional Information :
RRID:AB_528175
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Cell Biol. 100, 598-605.; Dev. Biol. 117, 90-101.; Zoology 107, 261-273.; J. Bone Miner. Res. 26(12), 2911-2922.; Dev. Cell 20, 764-774.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US