Dystroglycan, alpha- | Developmental Studies Hybridoma Bank VIA41 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Dystroglycan, alpha-

catalog :

VIA41

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

VIA41

reactivity :

mouse, rabbit

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 8	Inamori K, Willer T, Hara Y, Venzke D, Anderson M, Clarke N, et al. Endogenous glucuronyltransferase activity of LARGE or LARGE2 required for functional modification of ?-dystroglycan in cells and tissues. J Biol Chem. 2014;289:28138-48 pubmed publisher
	Jensen B, Willer T, Saade D, Cox M, Mozaffar T, Scavina M, et al. GMPPB-Associated Dystroglycanopathy: Emerging Common Variants with Phenotype Correlation. Hum Mutat. 2015;36:1159-63 pubmed publisher
	Raval K, Tao R, White B, de Lange W, Koonce C, Yu J, et al. Pompe disease results in a Golgi-based glycosylation deficit in human induced pluripotent stem cell-derived cardiomyocytes. J Biol Chem. 2015;290:3121-36 pubmed publisher
	Yang A, Ng B, Moore S, Rush J, Waechter C, Raymond K, et al. Congenital disorder of glycosylation due to DPM1 mutations presenting with dystroglycanopathy-type congenital muscular dystrophy. Mol Genet Metab. 2013;110:345-351 pubmed publisher
	Willer T, Lee H, Lommel M, Yoshida Moriguchi T, de Bernabe D, Venzke D, et al. ISPD loss-of-function mutations disrupt dystroglycan O-mannosylation and cause Walker-Warburg syndrome. Nat Genet. 2012;44:575-80 pubmed publisher
	Zhou Y, Munoz J, Jiang D, Jarrett H. Laminin-?1 LG4-5 domain binding to dystroglycan mediates muscle cell survival, growth, and the AP-1 and NF-?B transcription factors but also has adverse effects. Am J Physiol Cell Physiol. 2012;302:C902-14 pubmed publisher
	Zhang P, Hu H. Differential glycosylation of ?-dystroglycan and proteins other than ?-dystroglycan by like-glycosyltransferase. Glycobiology. 2012;22:235-47 pubmed publisher
	Michele D, Barresi R, Kanagawa M, Saito F, Cohn R, Satz J, et al. Post-translational disruption of dystroglycan-ligand interactions in congenital muscular dystrophies. Nature. 2002;418:417-22 pubmed
	Ervasti J, Ohlendieck K, Kahl S, Gaver M, Campbell K. Deficiency of a glycoprotein component of the dystrophin complex in dystrophic muscle. Nature. 1990;345:315-9 pubmed
	Ervasti J, Campbell K. Membrane organization of the dystrophin-glycoprotein complex. Cell. 1991;66:1121-31 pubmed
	Ohlendieck K, Campbell K. Dystrophin-associated proteins are greatly reduced in skeletal muscle from mdx mice. J Cell Biol. 1991;115:1685-94 pubmed

product information

Internal ID :

7766

Name :

VIA41

Depositor Name :

Campbell, K.P.

Depositor Institution :

University of Iowa

Date Deposited :

9/29/11

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

Dystroglycan, alpha-

Antigen Species :

Rabbit

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human,Mouse,Rabbit

Species Tested (legacy) :

rabbit, mouse, human

Initial Publication Pubmed ID :

2188135

Collections :

Human,Muscular dystrophy,Skeletal muscle

Search Keywords :

Campbell, K.P., Dystroglycan, alpha-, Rabbit, MIgG1, Rabbit/Mouse/Human, DAG1, , AB_2617217, monoclonal, Muscular dystrophy/Skeletal muscle/Human, Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Apparent: 156 kDa

Gene :

DAG1

Uniprot ID :

Q28685

Antibody Registry ID :

AB_2617217

Synonyms (Alternate Clone Names) :

VIA4-1

Immunogen :

Dystrophin complex isolated from detergent-solubilized skeletal muscle membranes using wheat-germ agglutinin-Sepharose.

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

2188135 22522420 12140558 26310427 23856421

Western Blot Pubmed IDs :

2188135 1913804 1757468 25138275 25488666 25138275 12140558

Immunoprecipitation Pubmed IDs :

22159078 21930648

Pubmed IDs :

25138275

Additional Information :

VIA41 recognizes a glycoconjugate on the LARGE fully glycosylated form of alphaDG. Dystroglycan is a central component of the dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. RRID:AB_2617217

DSHB Growth Medium :

RPMI

References (legacy) :

Cell 66, 1121-1131.; J. Cell Biol. 115, 1685-1694.; J. Biol. Chem. 278(18), 15457-15460.; J. Biol. Chem. doi: 10.1074/jbc.M114.597831.

more info or order :

Developmental Studies Hybridoma Bank product webpage