product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
fibronectin
catalog :
VA1(3)
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
VA1(3)
reactivity :
chicken
application :
western blot, immunocytochemistry
citations: 4
Reference
Dias A, de Almeida I, Belmonte J, Glazier J, Stern C. Somites without a clock. Science. 2014;343:791-795 pubmed publisher
Nakaya Y, Sukowati E, Wu Y, Sheng G. RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation. Nat Cell Biol. 2008;10:765-75 pubmed publisher
Honig M, Camilli S, Surineni K, Knight B, Hardin H. The contributions of BMP4, positive guidance cues, and repulsive molecules to cutaneous nerve formation in the chick hindlimb. Dev Biol. 2005;282:257-73 pubmed
Lefcort F, Venstrom K, McDonald J, Reichardt L. Regulation of expression of fibronectin and its receptor, alpha 5 beta 1, during development and regeneration of peripheral nerve. Development. 1992;116:767-82 pubmed
product information
Internal ID :
2261
Name :
VA1(3)
Depositor Name :
Solursh, M.
Depositor Institution :
University of Iowa
Date Deposited :
4/16/86
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
fibronectin
Antigen Species :
chicken
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Bovine,Chicken,Newt
Species Tested (legacy) :
positive on chicken negative on rat & frog.
Initial Publication Pubmed ID :
1289065
Depositor Notes (Special Instructions) :
Doesn't cross react with rat or frog fibronectin. VA1(3) recognizes intact FN as well as the 140/160 kDa cell binding fragments generated by elastase digestion.
Collections :
Cell adhesion,Cell signaling,Extracellular matrix proteins
Search Keywords :
Solursh, M., fibronectin, chicken, MIgG1, kappa light chain, Chicken/Newt/bovine, FN1, FN, AB_2105709, monoclonal, Cell adhesion/Extracellular matrix proteins/Cell signaling, Immunofluorescence/Western Blot
Antigen Molecular Weight :
Predicted: 270 kDa; Apparent: multiple isoforms around 230 kDa
Gene :
FN1
Alternate Gene Name(s) :
FN
Uniprot ID :
F1NJT3
Antibody Registry ID :
AB_2105709
Synonyms (Alternate Clone Names) :
VA1-3, VA1 3, VA13
Immunogen :
Undifferentiated mesenchyme and ectodermal sleeve from chicken wing bud (stage 23-24)
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
NS1
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Western Blot
Immunoblotting (legacy) :
Yes
Immunofluorescence Pubmed IDs :
1289065 24407478 18552836 15936345
Western Blot Pubmed IDs :
1289065
DSHB Growth Medium :
RPMI
References (legacy) :
Nat. Cell Biol. 10(7), 765-775.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US