product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
histone 2A gamma variant, phosphorylated
catalog :
UNC93-5.2.1
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
UNC93-5.2.1
reactivity :
fruit fly
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 9
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; fruit fly; 1:20; loading ...; fig 3a
Jones B, Wood J, Chang C, Tam A, Franklin M, Siegel E, et al. A somatic piRNA pathway in the Drosophila fat body ensures metabolic homeostasis and normal lifespan. Nat Commun. 2016;7:13856 pubmed publisher
  • immunohistochemistry - paraffin section; fruit fly; 1:500; fig 8
Tran M, Tsarouhas V, Kegel A. Early development of Drosophila embryos requires Smc5/6 function during oogenesis. Biol Open. 2016;5:928-41 pubmed publisher
  • immunocytochemistry; fruit fly; 1:100; fig 5
Ryu T, Spatola B, Delabaere L, Bowlin K, Hopp H, Kunitake R, et al. Heterochromatic breaks move to the nuclear periphery to continue recombinational repair. Nat Cell Biol. 2015;17:1401-11 pubmed publisher
Oberacker T, Bajorat J, Ziola S, Schroeder A, Roth D, Kastl L, et al. Enhanced expression of thioredoxin-interacting-protein regulates oxidative DNA damage and aging. FEBS Lett. 2018;592:2297-2307 pubmed publisher
Karam J, Parikh R, Nayak D, Rosenkranz D, Gangaraju V. Co-chaperone Hsp70/Hsp90-organizing protein (Hop) is required for transposon silencing and Piwi-interacting RNA (piRNA) biogenesis. J Biol Chem. 2017;292:6039-6046 pubmed publisher
Li Y, Armstrong R, Duronio R, MacAlpine D. Methylation of histone H4 lysine 20 by PR-Set7 ensures the integrity of late replicating sequence domains in Drosophila. Nucleic Acids Res. 2016;44:7204-18 pubmed publisher
Börner K, Jain D, Vazquez Pianzola P, Vengadasalam S, Steffen N, Fyodorov D, et al. A role for tuned levels of nucleosome remodeler subunit ACF1 during Drosophila oogenesis. Dev Biol. 2016;411:217-230 pubmed publisher
Nakamura M, Ohsawa S, Igaki T. Mitochondrial defects trigger proliferation of neighbouring cells via a senescence-associated secretory phenotype in Drosophila. Nat Commun. 2014;5:5264 pubmed publisher
Lake C, Holsclaw J, Bellendir S, Sekelsky J, Hawley R. The development of a monoclonal antibody recognizing the Drosophila melanogaster phosphorylated histone H2A variant (?-H2AV). G3 (Bethesda). 2013;3:1539-43 pubmed publisher
product information
Internal ID :
8985
Name :
UNC93-5.2.1
Depositor Name :
Hawley, R.S.
Depositor Institution :
Stowers Institute
Depositor Website :
http://www.stowers.org/faculty/hawley-lab
Date Deposited :
9/9/13
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
histone 2A gamma variant, phosphorylated
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG2b, kappa light chain
Isotype for catalog (legacy) :
IgG2b, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
23833215
Collections :
Drosophila antigens,Nucleus
Search Keywords :
Hawley, R.S., histone 2A gamma variant, phosphorylated, Drosophila, MIgG2b, kappa light chain, Drosophila, His2A, gamma-H2Av, , Monoclonal, Drosophila antigens/Nucleus, ELISA/Immunofluorescence/Western Blot
Antigen Molecular Weight :
15 kDa
Gene :
His2Av
Alternate Gene Name(s) :
gamma-H2Av
Uniprot ID :
P08985
Antibody Registry ID :
AB_2618077
Immunogen :
Phosphorylated peptide conjugated to KLH
Immunogen Sequence :
QDPQRKGNVILpSQAY (15 residues at C-terminus)
Clonality :
Monoclonal
Myeloma Strain :
P3X63-AG8.653
Epitope Mapped :
Yes
Epitope Location or Sequence :
C-terminus
Epitope Map PubMed ID :
23833215
Recommended Applications :
ELISA,Immunofluorescence,Western Blot
Immunoblotting (legacy) :
yes
Immunofluorescence Pubmed IDs :
23833215 26851213 27288507 25345385 26502056 30393075 30389853
Western Blot Pubmed IDs :
23833215
Chromatin Immunoprecipitation Pubmed IDs :
27131378
Additional Information :
FLYBASE: FBgn0001197
DSHB Growth Medium :
Iscove's
References (legacy) :
G3: Genes Genomes Genetics 3, 1539-1543.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US