product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
LAMP-2 (hamster)
catalog :
UH3
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
UH3
reactivity :
Chinese hamster
application :
western blot, immunocytochemistry, immunoprecipitation
more info or order :
citations: 7
| Reference |
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Paulusma C, Folmer D, Ho Mok K, de Waart D, Hilarius P, Verhoeven A, et al. ATP8B1 requires an accessory protein for endoplasmic reticulum exit and plasma membrane lipid flippase activity. Hepatology. 2008;47:268-78 pubmed
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Storch S, Pohl S, Braulke T. A dileucine motif and a cluster of acidic amino acids in the second cytoplasmic domain of the batten disease-related CLN3 protein are required for efficient lysosomal targeting. J Biol Chem. 2004;279:53625-34 pubmed
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Frolov A, Srivastava K, Daphna Iken D, Traub L, Schaffer J, Ory D. Cholesterol overload promotes morphogenesis of a Niemann-Pick C (NPC)-like compartment independent of inhibition of NPC1 or HE1/NPC2 function. J Biol Chem. 2001;276:46414-21 pubmed
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Zhang M, Dwyer N, Love D, Cooney A, Comly M, Neufeld E, et al. Cessation of rapid late endosomal tubulovesicular trafficking in Niemann-Pick type C1 disease. Proc Natl Acad Sci U S A. 2001;98:4466-71 pubmed
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Kundra R, Kornfeld S. Asparagine-linked oligosaccharides protect Lamp-1 and Lamp-2 from intracellular proteolysis. J Biol Chem. 1999;274:31039-46 pubmed
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Uthayakumar S, Granger B. Cell surface accumulation of overexpressed hamster lysosomal membrane glycoproteins. Cell Mol Biol Res. 1995;41:405-20 pubmed
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product information
Internal ID :
3676
Name :
UH3
Depositor Name :
Granger, B.L. / Uthayakumar, S.
Depositor Institution :
Montana State University
Date Deposited :
1/9/97
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
LAMP-2 (hamster)
Antigen Species :
Hamster
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Hamster
Species Tested (legacy) :
hamster, not mouse, rat, or monkey
Initial Publication Pubmed ID :
8867788
Depositor Notes (Special Instructions) :
UH1 recognizes native, methanol-fixed, and PLP-fixed hamster antigen. Doesn't recognize rat, mouse or monkey antigens. UH3 Labels presumptive lysosomes (and late endosomes). For best immunochemistry results, fix cells with methanol or with (para)formaldehyde-lysine-periodate formulation; for the latter, permeabilize with saponin, not with Triton X-100 or NP-40. Unfixed cells that have been permeabilized with saponin can also be stained. For immunoprecipitation, the antigen solubilized with Triton X-100 will bind to GL2A7 coupled to a matrix.
Collections :
CD antigens,Cell signaling
Search Keywords :
Granger, B.L. / Uthayakumar, S., LAMP-2 (hamster), Hamster, MIgG1, Hamster, LAMP2, CD107b, LGP-B, AB_528128, monoclonal, Cell signaling/Cluster Determinant antigens, Immunoprecipitation/Western Blot/Immunofluorescence
Antigen Molecular Weight :
Predicted: 42 kDa; Apparent: 110 kDa
Gene :
LAMP2
Alternate Gene Name(s) :
CD107b, LGP-B
Uniprot ID :
P49130
Antibody Registry ID :
AB_528128
Synonyms (Alternate Clone Names) :
CD107b
Immunogen :
Membrane glycoproteins pre-extracted with saponin from Chinese hamster ovary (CHO) cells and triton-X100-solubilized. Glycoproteins were affinity-purified by binding to wheat-germ agglutinin. The fractions eluting with N-acetyl D-glucosamine were used.
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
P3U1
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunoprecipitation,Western Blot
Immunofluorescence Pubmed IDs :
8867788 11296289 15469932 11571306 17948906 26545255
Western Blot Pubmed IDs :
10521503
Immunoprecipitation Pubmed IDs :
8867788
DSHB Growth Medium :
Iscove's
References (legacy) :
Cell. & Mol. Biol. Res. 41(5), 405-420.
more info or order :
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Developmental Studies Hybridoma Bank
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